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The P66Shc/Mitochondrial Permeability Transition Pore Pathway Determines Neurodegeneration

Mitochondrial-mediated oxidative stress and apoptosis play a crucial role in neurodegenerative disease and aging. Both mitochondrial permeability transition (PT) and swelling of mitochondria have been involved in neurodegeneration. Indeed, knockout mice for cyclophilin-D (Cyc-D), a key regulatory co...

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Autores principales: Savino, Costanza, Pelicci, PierGiuseppe, Giorgio, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671270/
https://www.ncbi.nlm.nih.gov/pubmed/23766859
http://dx.doi.org/10.1155/2013/719407
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author Savino, Costanza
Pelicci, PierGiuseppe
Giorgio, Marco
author_facet Savino, Costanza
Pelicci, PierGiuseppe
Giorgio, Marco
author_sort Savino, Costanza
collection PubMed
description Mitochondrial-mediated oxidative stress and apoptosis play a crucial role in neurodegenerative disease and aging. Both mitochondrial permeability transition (PT) and swelling of mitochondria have been involved in neurodegeneration. Indeed, knockout mice for cyclophilin-D (Cyc-D), a key regulatory component of the PT pore (PTP) that triggers mitochondrial swelling, resulted to be protected in preclinical models of multiple sclerosis (MS), Parkinson's disease (PD), and amyotrophic lateral sclerosis (ALS). However, how neuronal stress is transduced into mitochondrial oxidative stress and swelling is unclear. Recently, the aging determinant p66Shc that generates H(2)O(2) reacting with cytochrome c and induces oxidation of PTP and mitochondrial swelling was found to be involved in MS and ALS. To investigate the role of p66Shc/PTP pathway in neurodegeneration, we performed experimental autoimmune encephalomyelitis (EAE) experiments in p66Shc knockout mice (p66Shc−/−), knock out mice for cyclophilin-D (Cyc-D−/−), and p66Shc Cyc-D double knock out (p66Shc/Cyc-D−/−) mice. Results confirm that deletion of p66Shc protects from EAE without affecting immune response, whereas it is not epistatic to the Cyc-D mutation. These findings demonstrate that p66Shc contributes to EAE induced neuronal damage most likely through the opening of PTP suggesting that p66Shc/PTP pathway transduces neurodegenerative stresses.
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spelling pubmed-36712702013-06-13 The P66Shc/Mitochondrial Permeability Transition Pore Pathway Determines Neurodegeneration Savino, Costanza Pelicci, PierGiuseppe Giorgio, Marco Oxid Med Cell Longev Research Article Mitochondrial-mediated oxidative stress and apoptosis play a crucial role in neurodegenerative disease and aging. Both mitochondrial permeability transition (PT) and swelling of mitochondria have been involved in neurodegeneration. Indeed, knockout mice for cyclophilin-D (Cyc-D), a key regulatory component of the PT pore (PTP) that triggers mitochondrial swelling, resulted to be protected in preclinical models of multiple sclerosis (MS), Parkinson's disease (PD), and amyotrophic lateral sclerosis (ALS). However, how neuronal stress is transduced into mitochondrial oxidative stress and swelling is unclear. Recently, the aging determinant p66Shc that generates H(2)O(2) reacting with cytochrome c and induces oxidation of PTP and mitochondrial swelling was found to be involved in MS and ALS. To investigate the role of p66Shc/PTP pathway in neurodegeneration, we performed experimental autoimmune encephalomyelitis (EAE) experiments in p66Shc knockout mice (p66Shc−/−), knock out mice for cyclophilin-D (Cyc-D−/−), and p66Shc Cyc-D double knock out (p66Shc/Cyc-D−/−) mice. Results confirm that deletion of p66Shc protects from EAE without affecting immune response, whereas it is not epistatic to the Cyc-D mutation. These findings demonstrate that p66Shc contributes to EAE induced neuronal damage most likely through the opening of PTP suggesting that p66Shc/PTP pathway transduces neurodegenerative stresses. Hindawi Publishing Corporation 2013 2013-05-15 /pmc/articles/PMC3671270/ /pubmed/23766859 http://dx.doi.org/10.1155/2013/719407 Text en Copyright © 2013 Costanza Savino et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Savino, Costanza
Pelicci, PierGiuseppe
Giorgio, Marco
The P66Shc/Mitochondrial Permeability Transition Pore Pathway Determines Neurodegeneration
title The P66Shc/Mitochondrial Permeability Transition Pore Pathway Determines Neurodegeneration
title_full The P66Shc/Mitochondrial Permeability Transition Pore Pathway Determines Neurodegeneration
title_fullStr The P66Shc/Mitochondrial Permeability Transition Pore Pathway Determines Neurodegeneration
title_full_unstemmed The P66Shc/Mitochondrial Permeability Transition Pore Pathway Determines Neurodegeneration
title_short The P66Shc/Mitochondrial Permeability Transition Pore Pathway Determines Neurodegeneration
title_sort p66shc/mitochondrial permeability transition pore pathway determines neurodegeneration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671270/
https://www.ncbi.nlm.nih.gov/pubmed/23766859
http://dx.doi.org/10.1155/2013/719407
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