Identification of the causative agent of cutaneous leishmaniasis in Chichaoua province, Morocco

Cutaneous leishmaniasis (CL) in Morocco is caused by three species, Leishmania major, L. tropica and L. infantum. CL has been known in Chichaoua province since 2000. Using DNA extracted from microscopic slides and parasite cultures, collected in the years 2006 and 2009, we identified for the first t...

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Autores principales: Rhajaoui, M., Sebti, F., Fellah, H., Alam, M.Z., Nasereddin, A., Abbasi, I., Schönian, G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2012
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671429/
https://www.ncbi.nlm.nih.gov/pubmed/22314244
http://dx.doi.org/10.1051/parasite/2012191081
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author Rhajaoui, M.
Sebti, F.
Fellah, H.
Alam, M.Z.
Nasereddin, A.
Abbasi, I.
Schönian, G.
author_facet Rhajaoui, M.
Sebti, F.
Fellah, H.
Alam, M.Z.
Nasereddin, A.
Abbasi, I.
Schönian, G.
author_sort Rhajaoui, M.
collection PubMed
description Cutaneous leishmaniasis (CL) in Morocco is caused by three species, Leishmania major, L. tropica and L. infantum. CL has been known in Chichaoua province since 2000. Using DNA extracted from microscopic slides and parasite cultures, collected in the years 2006 and 2009, we identified for the first time L. tropica as the causative agent of CL in this region. Species identification was achieved by performing the ITS1-PCR-RFLP approach. By using this method it was possible to identify parasites in Giemsa stained slides containing less than five parasites per oil-immersion field even they were conserved for up to four months.
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spelling pubmed-36714292013-07-24 Identification of the causative agent of cutaneous leishmaniasis in Chichaoua province, Morocco Rhajaoui, M. Sebti, F. Fellah, H. Alam, M.Z. Nasereddin, A. Abbasi, I. Schönian, G. Parasite Research Note Cutaneous leishmaniasis (CL) in Morocco is caused by three species, Leishmania major, L. tropica and L. infantum. CL has been known in Chichaoua province since 2000. Using DNA extracted from microscopic slides and parasite cultures, collected in the years 2006 and 2009, we identified for the first time L. tropica as the causative agent of CL in this region. Species identification was achieved by performing the ITS1-PCR-RFLP approach. By using this method it was possible to identify parasites in Giemsa stained slides containing less than five parasites per oil-immersion field even they were conserved for up to four months. EDP Sciences 2012-02 2012-02-15 /pmc/articles/PMC3671429/ /pubmed/22314244 http://dx.doi.org/10.1051/parasite/2012191081 Text en © PRINCEPS Editions, Paris, 2012 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Note
Rhajaoui, M.
Sebti, F.
Fellah, H.
Alam, M.Z.
Nasereddin, A.
Abbasi, I.
Schönian, G.
Identification of the causative agent of cutaneous leishmaniasis in Chichaoua province, Morocco
title Identification of the causative agent of cutaneous leishmaniasis in Chichaoua province, Morocco
title_full Identification of the causative agent of cutaneous leishmaniasis in Chichaoua province, Morocco
title_fullStr Identification of the causative agent of cutaneous leishmaniasis in Chichaoua province, Morocco
title_full_unstemmed Identification of the causative agent of cutaneous leishmaniasis in Chichaoua province, Morocco
title_short Identification of the causative agent of cutaneous leishmaniasis in Chichaoua province, Morocco
title_sort identification of the causative agent of cutaneous leishmaniasis in chichaoua province, morocco
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671429/
https://www.ncbi.nlm.nih.gov/pubmed/22314244
http://dx.doi.org/10.1051/parasite/2012191081
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