Prevalence and molecular identification of Cryptosporidium isolates from pet lizards and snakes in Italy

In order to acquire prevalence and genetic data on Cryptosporidium infections in captive lizards and snakes kept as pets, a survey was conducted on 150 individual reptiles from southern Italy. Fecal samples were preserved in 5% formalin and analyzed using a commercial immunofluorescence assay (IFA)...

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Autores principales: Rinaldi, L., Capasso, M., Mihalca, A.D., Cirillo, R., Cringoli, G., Cacciò, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2012
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671454/
https://www.ncbi.nlm.nih.gov/pubmed/23193530
http://dx.doi.org/10.1051/parasite/2012194437
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author Rinaldi, L.
Capasso, M.
Mihalca, A.D.
Cirillo, R.
Cringoli, G.
Cacciò, S.
author_facet Rinaldi, L.
Capasso, M.
Mihalca, A.D.
Cirillo, R.
Cringoli, G.
Cacciò, S.
author_sort Rinaldi, L.
collection PubMed
description In order to acquire prevalence and genetic data on Cryptosporidium infections in captive lizards and snakes kept as pets, a survey was conducted on 150 individual reptiles from southern Italy. Fecal samples were preserved in 5% formalin and analyzed using a commercial immunofluorescence assay (IFA) for the detection of Cryptosporidium oocysts and Giardia cysts. IFA revealed the presence of Cryptosporidium oocysts in nine of the 150 samples examined (6.0%), precisely in 6/125 snakes (4.8%) and in 3/25 lizards (12.0%); all fecal samples tested negative for the presence of Giardia cysts. Molecular characterization based on nested PCR amplification and sequencing of the SSU-rRNA gene, revealed the presence of Cryptosporidium serpentis in three samples from snakes (Boa constrictor constrictor, Elapheguttata guttata guttata and Python molurus).
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spelling pubmed-36714542013-07-24 Prevalence and molecular identification of Cryptosporidium isolates from pet lizards and snakes in Italy Rinaldi, L. Capasso, M. Mihalca, A.D. Cirillo, R. Cringoli, G. Cacciò, S. Parasite Research Note In order to acquire prevalence and genetic data on Cryptosporidium infections in captive lizards and snakes kept as pets, a survey was conducted on 150 individual reptiles from southern Italy. Fecal samples were preserved in 5% formalin and analyzed using a commercial immunofluorescence assay (IFA) for the detection of Cryptosporidium oocysts and Giardia cysts. IFA revealed the presence of Cryptosporidium oocysts in nine of the 150 samples examined (6.0%), precisely in 6/125 snakes (4.8%) and in 3/25 lizards (12.0%); all fecal samples tested negative for the presence of Giardia cysts. Molecular characterization based on nested PCR amplification and sequencing of the SSU-rRNA gene, revealed the presence of Cryptosporidium serpentis in three samples from snakes (Boa constrictor constrictor, Elapheguttata guttata guttata and Python molurus). EDP Sciences 2012-11 2012-11-15 /pmc/articles/PMC3671454/ /pubmed/23193530 http://dx.doi.org/10.1051/parasite/2012194437 Text en © PRINCEPS Editions, Paris, 2012 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Note
Rinaldi, L.
Capasso, M.
Mihalca, A.D.
Cirillo, R.
Cringoli, G.
Cacciò, S.
Prevalence and molecular identification of Cryptosporidium isolates from pet lizards and snakes in Italy
title Prevalence and molecular identification of Cryptosporidium isolates from pet lizards and snakes in Italy
title_full Prevalence and molecular identification of Cryptosporidium isolates from pet lizards and snakes in Italy
title_fullStr Prevalence and molecular identification of Cryptosporidium isolates from pet lizards and snakes in Italy
title_full_unstemmed Prevalence and molecular identification of Cryptosporidium isolates from pet lizards and snakes in Italy
title_short Prevalence and molecular identification of Cryptosporidium isolates from pet lizards and snakes in Italy
title_sort prevalence and molecular identification of cryptosporidium isolates from pet lizards and snakes in italy
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671454/
https://www.ncbi.nlm.nih.gov/pubmed/23193530
http://dx.doi.org/10.1051/parasite/2012194437
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