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Effects of chemical combinations on the parthenogenetic activation of mouse oocytes

The aim of this study was to identify an optimal method for the parthenogenetic activation of mouse oocytes. Ethanol (EH), strontium chloride (SrCl(2)) and ionomycin calcium salt were each combined with cytochalasin B to induce the parthenogenetic activation of CD-1(®) mouse oocytes. Among the EH co...

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Autores principales: HAN, BAO-SHENG, GAO, JUN-LING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671782/
https://www.ncbi.nlm.nih.gov/pubmed/23737865
http://dx.doi.org/10.3892/etm.2013.1018
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author HAN, BAO-SHENG
GAO, JUN-LING
author_facet HAN, BAO-SHENG
GAO, JUN-LING
author_sort HAN, BAO-SHENG
collection PubMed
description The aim of this study was to identify an optimal method for the parthenogenetic activation of mouse oocytes. Ethanol (EH), strontium chloride (SrCl(2)) and ionomycin calcium salt were each combined with cytochalasin B to induce the parthenogenetic activation of CD-1(®) mouse oocytes. Among the EH combination groups, the blastocyst formation and hatching rates of the group that was activated with EH and CB for 5 min were significantly higher compared with those of the groups that were activated for 7 and 10 min (P<0.05). Among the SrCl(2) combination groups, the blastocyst formation and hatching rates of the group that was activated with SrCl(2) and CB for 30 min were significantly higher compared with those of the groups that were activated for 1 and 2 h (P<0.05). Among the ionomycin calcium salt combination groups, the blastocyst formation and hatching rates of the group that was activated with ionomycin and CB for 3 min were higher compared with those of the groups that were activated for 5 and 7 min (P<0.05). Compared with the other two combinations, the experimental indicators of the EH combination groups were notably superior (P<0.05). For combined activation, simultaneous activation with two substances was significantly more effective than successive activation (P<0.05). For combined activation with EH and cytochalasin B in mouse oocytes, 5 min of parthenogenetic activation had significant advantages with regard to cleavage, blastocyst formation and blastocyst hatching rates. In addition, the activation rate of combined activation was higher than that of single activators. For combined activation, the simultaneous application of two activators has a superior effect.
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spelling pubmed-36717822013-06-04 Effects of chemical combinations on the parthenogenetic activation of mouse oocytes HAN, BAO-SHENG GAO, JUN-LING Exp Ther Med Articles The aim of this study was to identify an optimal method for the parthenogenetic activation of mouse oocytes. Ethanol (EH), strontium chloride (SrCl(2)) and ionomycin calcium salt were each combined with cytochalasin B to induce the parthenogenetic activation of CD-1(®) mouse oocytes. Among the EH combination groups, the blastocyst formation and hatching rates of the group that was activated with EH and CB for 5 min were significantly higher compared with those of the groups that were activated for 7 and 10 min (P<0.05). Among the SrCl(2) combination groups, the blastocyst formation and hatching rates of the group that was activated with SrCl(2) and CB for 30 min were significantly higher compared with those of the groups that were activated for 1 and 2 h (P<0.05). Among the ionomycin calcium salt combination groups, the blastocyst formation and hatching rates of the group that was activated with ionomycin and CB for 3 min were higher compared with those of the groups that were activated for 5 and 7 min (P<0.05). Compared with the other two combinations, the experimental indicators of the EH combination groups were notably superior (P<0.05). For combined activation, simultaneous activation with two substances was significantly more effective than successive activation (P<0.05). For combined activation with EH and cytochalasin B in mouse oocytes, 5 min of parthenogenetic activation had significant advantages with regard to cleavage, blastocyst formation and blastocyst hatching rates. In addition, the activation rate of combined activation was higher than that of single activators. For combined activation, the simultaneous application of two activators has a superior effect. D.A. Spandidos 2013-05 2013-03-19 /pmc/articles/PMC3671782/ /pubmed/23737865 http://dx.doi.org/10.3892/etm.2013.1018 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
HAN, BAO-SHENG
GAO, JUN-LING
Effects of chemical combinations on the parthenogenetic activation of mouse oocytes
title Effects of chemical combinations on the parthenogenetic activation of mouse oocytes
title_full Effects of chemical combinations on the parthenogenetic activation of mouse oocytes
title_fullStr Effects of chemical combinations on the parthenogenetic activation of mouse oocytes
title_full_unstemmed Effects of chemical combinations on the parthenogenetic activation of mouse oocytes
title_short Effects of chemical combinations on the parthenogenetic activation of mouse oocytes
title_sort effects of chemical combinations on the parthenogenetic activation of mouse oocytes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671782/
https://www.ncbi.nlm.nih.gov/pubmed/23737865
http://dx.doi.org/10.3892/etm.2013.1018
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