Cargando…

Comparison of treatments for bullous keratopathy in rabbits

The aim of the present study was to compare deep lamellar endothelial keratoplasty (DLEK) and penetrating keratoplasty (PK) treatments for bullous keratopathy (BK). In total, 36 healthy New Zealand white rabbits were randomly divided into 3 groups termed the experimental, DLEK and PK groups. The exp...

Descripción completa

Detalles Bibliográficos
Autores principales: ZHAO, HAIXIA, LUO, YUNNA, NIU, CHUNMEI, GUAN, WENYING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671844/
https://www.ncbi.nlm.nih.gov/pubmed/23737903
http://dx.doi.org/10.3892/etm.2013.1025
_version_ 1782272046316650496
author ZHAO, HAIXIA
LUO, YUNNA
NIU, CHUNMEI
GUAN, WENYING
author_facet ZHAO, HAIXIA
LUO, YUNNA
NIU, CHUNMEI
GUAN, WENYING
author_sort ZHAO, HAIXIA
collection PubMed
description The aim of the present study was to compare deep lamellar endothelial keratoplasty (DLEK) and penetrating keratoplasty (PK) treatments for bullous keratopathy (BK). In total, 36 healthy New Zealand white rabbits were randomly divided into 3 groups termed the experimental, DLEK and PK groups. The experimental control group received no treatment. The DLEK and PK groups were observed for corneal astigmatism at 1, 2, or 3 months post-surgery using a corneal topography instrument and a slit lamp microscope. The incidence of immune rejection after 3 months of recovery was determined using hematoxylin and eosin (H&E) staining. The corneal specimens from the surgery groups were compared with those from the control group. In the 12 rabbit eyes that underwent the DLEK surgery, the central cornea became clear after 1 week. After 3 months, these corneas were almost transparent and no eye infections or other complications were observed in 10 of the eyes, while surgical perforations in 2 eyes led to surgical lamellar failure. In the PK surgery group, in which 12 rabbit eyes were also treated, nine were almost transparent after 3 months of recovery, while three eyes were immunologically rejected due to the corneal grafts. The occur-rences of corneal astigmatism that were observed following DLEK and PK treatment were significantly different after 1, 2 and 3 months of recovery (P<0.05). Normal corneal staining was observed in the DLEK and PK rabbits subjected to H&E staining after 3 months of recovery. A BK animal model was established by curetting the Descemet’s membrane (DM film). In comparison with PK, DLEK is a superior surgical treatment for BK.
format Online
Article
Text
id pubmed-3671844
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-36718442013-06-04 Comparison of treatments for bullous keratopathy in rabbits ZHAO, HAIXIA LUO, YUNNA NIU, CHUNMEI GUAN, WENYING Exp Ther Med Articles The aim of the present study was to compare deep lamellar endothelial keratoplasty (DLEK) and penetrating keratoplasty (PK) treatments for bullous keratopathy (BK). In total, 36 healthy New Zealand white rabbits were randomly divided into 3 groups termed the experimental, DLEK and PK groups. The experimental control group received no treatment. The DLEK and PK groups were observed for corneal astigmatism at 1, 2, or 3 months post-surgery using a corneal topography instrument and a slit lamp microscope. The incidence of immune rejection after 3 months of recovery was determined using hematoxylin and eosin (H&E) staining. The corneal specimens from the surgery groups were compared with those from the control group. In the 12 rabbit eyes that underwent the DLEK surgery, the central cornea became clear after 1 week. After 3 months, these corneas were almost transparent and no eye infections or other complications were observed in 10 of the eyes, while surgical perforations in 2 eyes led to surgical lamellar failure. In the PK surgery group, in which 12 rabbit eyes were also treated, nine were almost transparent after 3 months of recovery, while three eyes were immunologically rejected due to the corneal grafts. The occur-rences of corneal astigmatism that were observed following DLEK and PK treatment were significantly different after 1, 2 and 3 months of recovery (P<0.05). Normal corneal staining was observed in the DLEK and PK rabbits subjected to H&E staining after 3 months of recovery. A BK animal model was established by curetting the Descemet’s membrane (DM film). In comparison with PK, DLEK is a superior surgical treatment for BK. D.A. Spandidos 2013-05 2013-03-21 /pmc/articles/PMC3671844/ /pubmed/23737903 http://dx.doi.org/10.3892/etm.2013.1025 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
ZHAO, HAIXIA
LUO, YUNNA
NIU, CHUNMEI
GUAN, WENYING
Comparison of treatments for bullous keratopathy in rabbits
title Comparison of treatments for bullous keratopathy in rabbits
title_full Comparison of treatments for bullous keratopathy in rabbits
title_fullStr Comparison of treatments for bullous keratopathy in rabbits
title_full_unstemmed Comparison of treatments for bullous keratopathy in rabbits
title_short Comparison of treatments for bullous keratopathy in rabbits
title_sort comparison of treatments for bullous keratopathy in rabbits
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671844/
https://www.ncbi.nlm.nih.gov/pubmed/23737903
http://dx.doi.org/10.3892/etm.2013.1025
work_keys_str_mv AT zhaohaixia comparisonoftreatmentsforbullouskeratopathyinrabbits
AT luoyunna comparisonoftreatmentsforbullouskeratopathyinrabbits
AT niuchunmei comparisonoftreatmentsforbullouskeratopathyinrabbits
AT guanwenying comparisonoftreatmentsforbullouskeratopathyinrabbits