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Identification of a Testis-Enriched Heat Shock Protein and Fourteen Members of Hsp70 Family in the Swamp Eel

BACKGROUND: Gonad differentiation is one of the most important developmental events in vertebrates. Some heat shock proteins are associated with gonad development. Heat shock protein 70 (Hsp70) in the teleost fish and its roles in sex differentiation are poorly understood. METHODS AND FINDINGS: We h...

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Autores principales: He, Yan, Luo, Majing, Yi, Minhan, Sheng, Yue, Cheng, Yibin, Zhou, Rongjia, Cheng, Hanhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672149/
https://www.ncbi.nlm.nih.gov/pubmed/23750249
http://dx.doi.org/10.1371/journal.pone.0065269
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author He, Yan
Luo, Majing
Yi, Minhan
Sheng, Yue
Cheng, Yibin
Zhou, Rongjia
Cheng, Hanhua
author_facet He, Yan
Luo, Majing
Yi, Minhan
Sheng, Yue
Cheng, Yibin
Zhou, Rongjia
Cheng, Hanhua
author_sort He, Yan
collection PubMed
description BACKGROUND: Gonad differentiation is one of the most important developmental events in vertebrates. Some heat shock proteins are associated with gonad development. Heat shock protein 70 (Hsp70) in the teleost fish and its roles in sex differentiation are poorly understood. METHODS AND FINDINGS: We have identified a testis-enriched heat shock protein Hspa8b2 in the swamp eel using Western blot analysis and Mass Spectrometry (MS). Fourteen Hsp70 family genes were further identified in this species based on transcriptome information. The phylogenetic tree of Hsp70 family was constructed using the Maximum Likelihood method and their expression patterns in the swamp eel gonads were analyzed by reverse transcription-polymerase chain reaction (RT-PCR). CONCLUSION: There are fourteen gene members in the Hsp70 family in the swamp eel genome. Hsp70 family, particularly Hspa8, has expanded in the species. One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.
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spelling pubmed-36721492013-06-07 Identification of a Testis-Enriched Heat Shock Protein and Fourteen Members of Hsp70 Family in the Swamp Eel He, Yan Luo, Majing Yi, Minhan Sheng, Yue Cheng, Yibin Zhou, Rongjia Cheng, Hanhua PLoS One Research Article BACKGROUND: Gonad differentiation is one of the most important developmental events in vertebrates. Some heat shock proteins are associated with gonad development. Heat shock protein 70 (Hsp70) in the teleost fish and its roles in sex differentiation are poorly understood. METHODS AND FINDINGS: We have identified a testis-enriched heat shock protein Hspa8b2 in the swamp eel using Western blot analysis and Mass Spectrometry (MS). Fourteen Hsp70 family genes were further identified in this species based on transcriptome information. The phylogenetic tree of Hsp70 family was constructed using the Maximum Likelihood method and their expression patterns in the swamp eel gonads were analyzed by reverse transcription-polymerase chain reaction (RT-PCR). CONCLUSION: There are fourteen gene members in the Hsp70 family in the swamp eel genome. Hsp70 family, particularly Hspa8, has expanded in the species. One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel. Public Library of Science 2013-06-04 /pmc/articles/PMC3672149/ /pubmed/23750249 http://dx.doi.org/10.1371/journal.pone.0065269 Text en © 2013 He et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
He, Yan
Luo, Majing
Yi, Minhan
Sheng, Yue
Cheng, Yibin
Zhou, Rongjia
Cheng, Hanhua
Identification of a Testis-Enriched Heat Shock Protein and Fourteen Members of Hsp70 Family in the Swamp Eel
title Identification of a Testis-Enriched Heat Shock Protein and Fourteen Members of Hsp70 Family in the Swamp Eel
title_full Identification of a Testis-Enriched Heat Shock Protein and Fourteen Members of Hsp70 Family in the Swamp Eel
title_fullStr Identification of a Testis-Enriched Heat Shock Protein and Fourteen Members of Hsp70 Family in the Swamp Eel
title_full_unstemmed Identification of a Testis-Enriched Heat Shock Protein and Fourteen Members of Hsp70 Family in the Swamp Eel
title_short Identification of a Testis-Enriched Heat Shock Protein and Fourteen Members of Hsp70 Family in the Swamp Eel
title_sort identification of a testis-enriched heat shock protein and fourteen members of hsp70 family in the swamp eel
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672149/
https://www.ncbi.nlm.nih.gov/pubmed/23750249
http://dx.doi.org/10.1371/journal.pone.0065269
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