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Targeting Aurora B to the Equatorial Cortex by MKlp2 Is Required for Cytokinesis

Although Aurora B is important in cleavage furrow ingression and completion during cytokinesis, the mechanism by which kinase activity is targeted to the cleavage furrow and the molecule(s) responsible for this process have remained elusive. Here, we demonstrate that an essential mitotic kinesin MKl...

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Autores principales: Kitagawa, Mayumi, Fung, Suet Yin Sarah, Onishi, Nobuyuki, Saya, Hideyuki, Lee, Sang Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672163/
https://www.ncbi.nlm.nih.gov/pubmed/23750214
http://dx.doi.org/10.1371/journal.pone.0064826
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author Kitagawa, Mayumi
Fung, Suet Yin Sarah
Onishi, Nobuyuki
Saya, Hideyuki
Lee, Sang Hyun
author_facet Kitagawa, Mayumi
Fung, Suet Yin Sarah
Onishi, Nobuyuki
Saya, Hideyuki
Lee, Sang Hyun
author_sort Kitagawa, Mayumi
collection PubMed
description Although Aurora B is important in cleavage furrow ingression and completion during cytokinesis, the mechanism by which kinase activity is targeted to the cleavage furrow and the molecule(s) responsible for this process have remained elusive. Here, we demonstrate that an essential mitotic kinesin MKlp2 requires myosin-II for its localization to the equatorial cortex, and this event is required to recruit Aurora B to the equatorial cortex in mammalian cells. This recruitment event is also required to promote the highly focused accumulation of active RhoA at the equatorial cortex and stable ingression of the cleavage furrow in bipolar cytokinesis. Specifically, in drug-induced monopolar cytokinesis, targeting Aurora B to the cell cortex by MKlp2 is essential for cell polarization and furrow formation. Once the furrow has formed, MKlp2 further recruits Aurora B to the growing furrow. This process together with continuous Aurora B kinase activity at the growing furrow is essential for stable furrow propagation and completion. In contrast, a MKlp2 mutant defective in binding myosin-II does not recruit Aurora B to the cell cortex and does not promote furrow formation during monopolar cytokinesis. This mutant is also defective in maintaining the ingressing furrow during bipolar cytokinesis. Together, these findings reveal that targeting Aurora B to the cell cortex (or the equatorial cortex) by MKlp2 is essential for the maintenance of the ingressing furrow for successful cytokinesis.
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spelling pubmed-36721632013-06-07 Targeting Aurora B to the Equatorial Cortex by MKlp2 Is Required for Cytokinesis Kitagawa, Mayumi Fung, Suet Yin Sarah Onishi, Nobuyuki Saya, Hideyuki Lee, Sang Hyun PLoS One Research Article Although Aurora B is important in cleavage furrow ingression and completion during cytokinesis, the mechanism by which kinase activity is targeted to the cleavage furrow and the molecule(s) responsible for this process have remained elusive. Here, we demonstrate that an essential mitotic kinesin MKlp2 requires myosin-II for its localization to the equatorial cortex, and this event is required to recruit Aurora B to the equatorial cortex in mammalian cells. This recruitment event is also required to promote the highly focused accumulation of active RhoA at the equatorial cortex and stable ingression of the cleavage furrow in bipolar cytokinesis. Specifically, in drug-induced monopolar cytokinesis, targeting Aurora B to the cell cortex by MKlp2 is essential for cell polarization and furrow formation. Once the furrow has formed, MKlp2 further recruits Aurora B to the growing furrow. This process together with continuous Aurora B kinase activity at the growing furrow is essential for stable furrow propagation and completion. In contrast, a MKlp2 mutant defective in binding myosin-II does not recruit Aurora B to the cell cortex and does not promote furrow formation during monopolar cytokinesis. This mutant is also defective in maintaining the ingressing furrow during bipolar cytokinesis. Together, these findings reveal that targeting Aurora B to the cell cortex (or the equatorial cortex) by MKlp2 is essential for the maintenance of the ingressing furrow for successful cytokinesis. Public Library of Science 2013-06-04 /pmc/articles/PMC3672163/ /pubmed/23750214 http://dx.doi.org/10.1371/journal.pone.0064826 Text en © 2013 Kitagawa et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kitagawa, Mayumi
Fung, Suet Yin Sarah
Onishi, Nobuyuki
Saya, Hideyuki
Lee, Sang Hyun
Targeting Aurora B to the Equatorial Cortex by MKlp2 Is Required for Cytokinesis
title Targeting Aurora B to the Equatorial Cortex by MKlp2 Is Required for Cytokinesis
title_full Targeting Aurora B to the Equatorial Cortex by MKlp2 Is Required for Cytokinesis
title_fullStr Targeting Aurora B to the Equatorial Cortex by MKlp2 Is Required for Cytokinesis
title_full_unstemmed Targeting Aurora B to the Equatorial Cortex by MKlp2 Is Required for Cytokinesis
title_short Targeting Aurora B to the Equatorial Cortex by MKlp2 Is Required for Cytokinesis
title_sort targeting aurora b to the equatorial cortex by mklp2 is required for cytokinesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672163/
https://www.ncbi.nlm.nih.gov/pubmed/23750214
http://dx.doi.org/10.1371/journal.pone.0064826
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