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Small chromosomes among Danish Candida glabrata isolates originated through different mechanisms

We analyzed 192 strains of the pathogenic yeast Candida glabrata from patients, mainly suffering from systemic infection, at Danish hospitals during 1985–1999. Our analysis showed that these strains were closely related but exhibited large karyotype polymorphism. Nine strains contained small chromos...

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Autores principales: Ahmad, Khadija Mohamed, Ishchuk, Olena P., Hellborg, Linda, Jørgensen, Gloria, Skvarc, Miha, Stenderup, Jørgen, Jørck-Ramberg, Dorte, Polakova, Silvia, Piškur, Jure
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672514/
https://www.ncbi.nlm.nih.gov/pubmed/23670790
http://dx.doi.org/10.1007/s10482-013-9931-3
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author Ahmad, Khadija Mohamed
Ishchuk, Olena P.
Hellborg, Linda
Jørgensen, Gloria
Skvarc, Miha
Stenderup, Jørgen
Jørck-Ramberg, Dorte
Polakova, Silvia
Piškur, Jure
author_facet Ahmad, Khadija Mohamed
Ishchuk, Olena P.
Hellborg, Linda
Jørgensen, Gloria
Skvarc, Miha
Stenderup, Jørgen
Jørck-Ramberg, Dorte
Polakova, Silvia
Piškur, Jure
author_sort Ahmad, Khadija Mohamed
collection PubMed
description We analyzed 192 strains of the pathogenic yeast Candida glabrata from patients, mainly suffering from systemic infection, at Danish hospitals during 1985–1999. Our analysis showed that these strains were closely related but exhibited large karyotype polymorphism. Nine strains contained small chromosomes, which were smaller than 0.5 Mb. Regarding the year, patient and hospital, these C. glabrata strains had independent origin and the analyzed small chromosomes were structurally not related to each other (i.e. they contained different sets of genes). We suggest that at least two mechanisms could participate in their origin: (i) through a segmental duplication which covered the centromeric region, or (ii) by a translocation event moving a larger chromosome arm to another chromosome that leaves the centromere part with the shorter arm. The first type of small chromosomes carrying duplicated genes exhibited mitotic instability, while the second type, which contained the corresponding genes in only one copy in the genome, was mitotically stable. Apparently, in patients C. glabrata chromosomes are frequently reshuffled resulting in new genetic configurations, including appearance of small chromosomes, and some of these resulting “mutant” strains can have increased fitness in a certain patient “environment”. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10482-013-9931-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-36725142013-06-10 Small chromosomes among Danish Candida glabrata isolates originated through different mechanisms Ahmad, Khadija Mohamed Ishchuk, Olena P. Hellborg, Linda Jørgensen, Gloria Skvarc, Miha Stenderup, Jørgen Jørck-Ramberg, Dorte Polakova, Silvia Piškur, Jure Antonie Van Leeuwenhoek Original Paper We analyzed 192 strains of the pathogenic yeast Candida glabrata from patients, mainly suffering from systemic infection, at Danish hospitals during 1985–1999. Our analysis showed that these strains were closely related but exhibited large karyotype polymorphism. Nine strains contained small chromosomes, which were smaller than 0.5 Mb. Regarding the year, patient and hospital, these C. glabrata strains had independent origin and the analyzed small chromosomes were structurally not related to each other (i.e. they contained different sets of genes). We suggest that at least two mechanisms could participate in their origin: (i) through a segmental duplication which covered the centromeric region, or (ii) by a translocation event moving a larger chromosome arm to another chromosome that leaves the centromere part with the shorter arm. The first type of small chromosomes carrying duplicated genes exhibited mitotic instability, while the second type, which contained the corresponding genes in only one copy in the genome, was mitotically stable. Apparently, in patients C. glabrata chromosomes are frequently reshuffled resulting in new genetic configurations, including appearance of small chromosomes, and some of these resulting “mutant” strains can have increased fitness in a certain patient “environment”. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10482-013-9931-3) contains supplementary material, which is available to authorized users. Springer Netherlands 2013-05-14 2013 /pmc/articles/PMC3672514/ /pubmed/23670790 http://dx.doi.org/10.1007/s10482-013-9931-3 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Paper
Ahmad, Khadija Mohamed
Ishchuk, Olena P.
Hellborg, Linda
Jørgensen, Gloria
Skvarc, Miha
Stenderup, Jørgen
Jørck-Ramberg, Dorte
Polakova, Silvia
Piškur, Jure
Small chromosomes among Danish Candida glabrata isolates originated through different mechanisms
title Small chromosomes among Danish Candida glabrata isolates originated through different mechanisms
title_full Small chromosomes among Danish Candida glabrata isolates originated through different mechanisms
title_fullStr Small chromosomes among Danish Candida glabrata isolates originated through different mechanisms
title_full_unstemmed Small chromosomes among Danish Candida glabrata isolates originated through different mechanisms
title_short Small chromosomes among Danish Candida glabrata isolates originated through different mechanisms
title_sort small chromosomes among danish candida glabrata isolates originated through different mechanisms
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672514/
https://www.ncbi.nlm.nih.gov/pubmed/23670790
http://dx.doi.org/10.1007/s10482-013-9931-3
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