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OPN gene polymorphisms influence the risk of knee OA and OPN levels in synovial fluid in a Chinese population

INTRODUCTION: A body of studies suggests the role of osteopontin (OPN) in onset and development of osteoarthritis (OA), however, the association between OPN polymorphisms and OA susceptibility as well as its clinical features has not been reported. METHODS: A total of 750 patients with primary knee...

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Detalles Bibliográficos
Autores principales: Jiang, Yongqing, Yao, Meng, Liu, Qingpeng, Zhou, Changwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672660/
https://www.ncbi.nlm.nih.gov/pubmed/23289928
http://dx.doi.org/10.1186/ar4129
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author Jiang, Yongqing
Yao, Meng
Liu, Qingpeng
Zhou, Changwei
author_facet Jiang, Yongqing
Yao, Meng
Liu, Qingpeng
Zhou, Changwei
author_sort Jiang, Yongqing
collection PubMed
description INTRODUCTION: A body of studies suggests the role of osteopontin (OPN) in onset and development of osteoarthritis (OA), however, the association between OPN polymorphisms and OA susceptibility as well as its clinical features has not been reported. METHODS: A total of 750 patients with primary knee OA and 794 healthy volunteer were enrolled as controls. Both OA and control groups were interviewed to obtain demographic and clinical data. Three polymorphisms of OPN gene, namely, -156GG/G, -443C/T and -66T/G were determined. The levels of the full length and the thrombin-cleaved OPN in synovial fluid (SF) from OA subjects were measured. RESULTS: We found the polymorphisms of the -443C/T and the -66/T/G were significantly associated with the OA risk and the radiographic severity. The -443TT and -66GG showed protective effect against developing OA and were associated with lower Kellgren-Lawrence grade. Besides, the polymorphisms of -443C/T and -66T/G significantly affected the thrombin-cleaved OPN levels in SF from OA subjects. Subjects with -443TT and -66GG genotypes had lower thrombin-cleaved OPN levels in SF. The thrombin-cleaved OPN levels in SF were positively correlated to the radiographic severity of OA. CONCLUSIONS: Our findings suggest that certain OPN gene polymorphisms may be used as molecular markers for the susceptibility and severity of OA.
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spelling pubmed-36726602013-06-06 OPN gene polymorphisms influence the risk of knee OA and OPN levels in synovial fluid in a Chinese population Jiang, Yongqing Yao, Meng Liu, Qingpeng Zhou, Changwei Arthritis Res Ther Research Article INTRODUCTION: A body of studies suggests the role of osteopontin (OPN) in onset and development of osteoarthritis (OA), however, the association between OPN polymorphisms and OA susceptibility as well as its clinical features has not been reported. METHODS: A total of 750 patients with primary knee OA and 794 healthy volunteer were enrolled as controls. Both OA and control groups were interviewed to obtain demographic and clinical data. Three polymorphisms of OPN gene, namely, -156GG/G, -443C/T and -66T/G were determined. The levels of the full length and the thrombin-cleaved OPN in synovial fluid (SF) from OA subjects were measured. RESULTS: We found the polymorphisms of the -443C/T and the -66/T/G were significantly associated with the OA risk and the radiographic severity. The -443TT and -66GG showed protective effect against developing OA and were associated with lower Kellgren-Lawrence grade. Besides, the polymorphisms of -443C/T and -66T/G significantly affected the thrombin-cleaved OPN levels in SF from OA subjects. Subjects with -443TT and -66GG genotypes had lower thrombin-cleaved OPN levels in SF. The thrombin-cleaved OPN levels in SF were positively correlated to the radiographic severity of OA. CONCLUSIONS: Our findings suggest that certain OPN gene polymorphisms may be used as molecular markers for the susceptibility and severity of OA. BioMed Central 2013 2013-01-05 /pmc/articles/PMC3672660/ /pubmed/23289928 http://dx.doi.org/10.1186/ar4129 Text en Copyright © 2013 Jiang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jiang, Yongqing
Yao, Meng
Liu, Qingpeng
Zhou, Changwei
OPN gene polymorphisms influence the risk of knee OA and OPN levels in synovial fluid in a Chinese population
title OPN gene polymorphisms influence the risk of knee OA and OPN levels in synovial fluid in a Chinese population
title_full OPN gene polymorphisms influence the risk of knee OA and OPN levels in synovial fluid in a Chinese population
title_fullStr OPN gene polymorphisms influence the risk of knee OA and OPN levels in synovial fluid in a Chinese population
title_full_unstemmed OPN gene polymorphisms influence the risk of knee OA and OPN levels in synovial fluid in a Chinese population
title_short OPN gene polymorphisms influence the risk of knee OA and OPN levels in synovial fluid in a Chinese population
title_sort opn gene polymorphisms influence the risk of knee oa and opn levels in synovial fluid in a chinese population
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672660/
https://www.ncbi.nlm.nih.gov/pubmed/23289928
http://dx.doi.org/10.1186/ar4129
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