Cargando…
Disease severity in K/BxN serum transfer-induced arthritis is not affected by IL-33 deficiency
INTRODUCTION: Interleukin (IL)-33 is a cytokine of the IL-1 family, which signals through the ST2 receptor. Previous work suggested implication of the IL-33/ST2 axis in the pathogenesis of human and mouse arthritis. Here, we directly investigated the role of endogenous IL-33 in K/BxN serum transfer-...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672723/ https://www.ncbi.nlm.nih.gov/pubmed/23324173 http://dx.doi.org/10.1186/ar4143 |
_version_ | 1782272160618774528 |
---|---|
author | Martin, Praxedis Talabot-Ayer, Dominique Seemayer, Christian Alexander Vigne, Solenne Lamacchia, Céline Rodriguez, Emiliana Finckh, Axel Smith, Dirk E Gabay, Cem Palmer, Gaby |
author_facet | Martin, Praxedis Talabot-Ayer, Dominique Seemayer, Christian Alexander Vigne, Solenne Lamacchia, Céline Rodriguez, Emiliana Finckh, Axel Smith, Dirk E Gabay, Cem Palmer, Gaby |
author_sort | Martin, Praxedis |
collection | PubMed |
description | INTRODUCTION: Interleukin (IL)-33 is a cytokine of the IL-1 family, which signals through the ST2 receptor. Previous work suggested implication of the IL-33/ST2 axis in the pathogenesis of human and mouse arthritis. Here, we directly investigated the role of endogenous IL-33 in K/BxN serum transfer-induced arthritis by using IL-33 knockout (KO) mice. METHODS: Arthritis was induced by injection of complete K/BxN serum or purified IgG. Disease severity was monitored by clinical and histological scoring. RESULTS: K/BxN serum transfer induced pronounced arthritis with similar incidence and severity in IL-33 KO and wild-type (WT) mice. In contrast, disease development was significantly reduced in ST2 KO mice. IL-33 expression in synovial tissue was comparable in arthritic WT and ST2 KO mice, and absent in IL-33 KO mice. Transfer of purified arthritogenic IgG instead of complete K/BxN serum also resulted in similar arthritis severity in IL-33 KO and WT mice, excluding a contribution of IL-33 contained in the serum of donor mice to explain this result. We investigated additional potential confounding factors, including purity of genetic background, but the mechanisms underlying reduced arthritis in ST2 KO mice remained unclear. CONCLUSIONS: The data obtained with IL-33 KO mice indicate that endogenous IL-33 is not required for the development of joint inflammation in K/BxN serum transfer-induced arthritis. On the contrary, arthritis severity was reduced in ST2 KO mice. This observation might relate to IL-33 independent effects of ST2, and/or reveal the existence of confounding variables affecting the severity of joint inflammation in these KO strains. |
format | Online Article Text |
id | pubmed-3672723 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-36727232013-06-10 Disease severity in K/BxN serum transfer-induced arthritis is not affected by IL-33 deficiency Martin, Praxedis Talabot-Ayer, Dominique Seemayer, Christian Alexander Vigne, Solenne Lamacchia, Céline Rodriguez, Emiliana Finckh, Axel Smith, Dirk E Gabay, Cem Palmer, Gaby Arthritis Res Ther Research Article INTRODUCTION: Interleukin (IL)-33 is a cytokine of the IL-1 family, which signals through the ST2 receptor. Previous work suggested implication of the IL-33/ST2 axis in the pathogenesis of human and mouse arthritis. Here, we directly investigated the role of endogenous IL-33 in K/BxN serum transfer-induced arthritis by using IL-33 knockout (KO) mice. METHODS: Arthritis was induced by injection of complete K/BxN serum or purified IgG. Disease severity was monitored by clinical and histological scoring. RESULTS: K/BxN serum transfer induced pronounced arthritis with similar incidence and severity in IL-33 KO and wild-type (WT) mice. In contrast, disease development was significantly reduced in ST2 KO mice. IL-33 expression in synovial tissue was comparable in arthritic WT and ST2 KO mice, and absent in IL-33 KO mice. Transfer of purified arthritogenic IgG instead of complete K/BxN serum also resulted in similar arthritis severity in IL-33 KO and WT mice, excluding a contribution of IL-33 contained in the serum of donor mice to explain this result. We investigated additional potential confounding factors, including purity of genetic background, but the mechanisms underlying reduced arthritis in ST2 KO mice remained unclear. CONCLUSIONS: The data obtained with IL-33 KO mice indicate that endogenous IL-33 is not required for the development of joint inflammation in K/BxN serum transfer-induced arthritis. On the contrary, arthritis severity was reduced in ST2 KO mice. This observation might relate to IL-33 independent effects of ST2, and/or reveal the existence of confounding variables affecting the severity of joint inflammation in these KO strains. BioMed Central 2013 2013-01-16 /pmc/articles/PMC3672723/ /pubmed/23324173 http://dx.doi.org/10.1186/ar4143 Text en Copyright © 2013 Martin et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Martin, Praxedis Talabot-Ayer, Dominique Seemayer, Christian Alexander Vigne, Solenne Lamacchia, Céline Rodriguez, Emiliana Finckh, Axel Smith, Dirk E Gabay, Cem Palmer, Gaby Disease severity in K/BxN serum transfer-induced arthritis is not affected by IL-33 deficiency |
title | Disease severity in K/BxN serum transfer-induced arthritis is not affected by IL-33 deficiency |
title_full | Disease severity in K/BxN serum transfer-induced arthritis is not affected by IL-33 deficiency |
title_fullStr | Disease severity in K/BxN serum transfer-induced arthritis is not affected by IL-33 deficiency |
title_full_unstemmed | Disease severity in K/BxN serum transfer-induced arthritis is not affected by IL-33 deficiency |
title_short | Disease severity in K/BxN serum transfer-induced arthritis is not affected by IL-33 deficiency |
title_sort | disease severity in k/bxn serum transfer-induced arthritis is not affected by il-33 deficiency |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672723/ https://www.ncbi.nlm.nih.gov/pubmed/23324173 http://dx.doi.org/10.1186/ar4143 |
work_keys_str_mv | AT martinpraxedis diseaseseverityinkbxnserumtransferinducedarthritisisnotaffectedbyil33deficiency AT talabotayerdominique diseaseseverityinkbxnserumtransferinducedarthritisisnotaffectedbyil33deficiency AT seemayerchristianalexander diseaseseverityinkbxnserumtransferinducedarthritisisnotaffectedbyil33deficiency AT vignesolenne diseaseseverityinkbxnserumtransferinducedarthritisisnotaffectedbyil33deficiency AT lamacchiaceline diseaseseverityinkbxnserumtransferinducedarthritisisnotaffectedbyil33deficiency AT rodriguezemiliana diseaseseverityinkbxnserumtransferinducedarthritisisnotaffectedbyil33deficiency AT finckhaxel diseaseseverityinkbxnserumtransferinducedarthritisisnotaffectedbyil33deficiency AT smithdirke diseaseseverityinkbxnserumtransferinducedarthritisisnotaffectedbyil33deficiency AT gabaycem diseaseseverityinkbxnserumtransferinducedarthritisisnotaffectedbyil33deficiency AT palmergaby diseaseseverityinkbxnserumtransferinducedarthritisisnotaffectedbyil33deficiency |