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Osthole Attenuates Hepatic Injury in a Rodent Model of Trauma-Hemorrhage

Recent evidences show that osthole possesses anti-inflammatory properties and protective effects following shock-like states, but the mechanism of these effects remains unknown. The p38 mitogen-activated protein kinase (p38 MAPK) pathway exerts anti-inflammatory effects in injury. The aim of this st...

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Autores principales: Yu, Huang-Ping, Liu, Fu-Chao, Tsai, Yung-Fong, Hwang, Tsong-Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675024/
https://www.ncbi.nlm.nih.gov/pubmed/23755293
http://dx.doi.org/10.1371/journal.pone.0065916
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author Yu, Huang-Ping
Liu, Fu-Chao
Tsai, Yung-Fong
Hwang, Tsong-Long
author_facet Yu, Huang-Ping
Liu, Fu-Chao
Tsai, Yung-Fong
Hwang, Tsong-Long
author_sort Yu, Huang-Ping
collection PubMed
description Recent evidences show that osthole possesses anti-inflammatory properties and protective effects following shock-like states, but the mechanism of these effects remains unknown. The p38 mitogen-activated protein kinase (p38 MAPK) pathway exerts anti-inflammatory effects in injury. The aim of this study was to investigate whether p38 MAPK plays any role in the osthole-mediated attenuation of hepatic injury after trauma-hemorrhage. Male Sprague-Dawley rats underwent trauma-hemorrhage (mean blood pressure maintained at approximately 35–40 mmHg for 90 minutes), followed by fluid resuscitation. During resuscitation, a single dose of osthole (3 mg/kg, intravenously) with and without a p38 MAPK inhibitor SB-203580 (2 mg/kg, intravenously), SB-203580 or vehicle was administered. Plasma alanine aminotransferase (ALT) with aspartate aminotransferase (AST) concentrations and various hepatic parameters were measured (n = 8 rats/group) at 24 hours after resuscitation. The results showed that trauma-hemorrhage increased hepatic myeloperoxidase activity, intercellular adhesion molecule-1 and interleukin-6 levels, and plasma ALT and AST concentrations. These parameters were significantly improved in the osthole-treated rats subjected to trauma-hemorrhage. Osthole treatment also increased hepatic phospho-p38 MAPK expression compared with vehicle-treated trauma-hemorrhaged rats. Co-administration of SB-203580 with osthole abolished the osthole-induced beneficial effects on the above parameters and hepatic injury. These results suggest that the protective effect of osthole administration on alleviation of hepatic injury after trauma-hemorrhage, which is, at least in part, through p38 MAPK-dependent pathway.
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spelling pubmed-36750242013-06-10 Osthole Attenuates Hepatic Injury in a Rodent Model of Trauma-Hemorrhage Yu, Huang-Ping Liu, Fu-Chao Tsai, Yung-Fong Hwang, Tsong-Long PLoS One Research Article Recent evidences show that osthole possesses anti-inflammatory properties and protective effects following shock-like states, but the mechanism of these effects remains unknown. The p38 mitogen-activated protein kinase (p38 MAPK) pathway exerts anti-inflammatory effects in injury. The aim of this study was to investigate whether p38 MAPK plays any role in the osthole-mediated attenuation of hepatic injury after trauma-hemorrhage. Male Sprague-Dawley rats underwent trauma-hemorrhage (mean blood pressure maintained at approximately 35–40 mmHg for 90 minutes), followed by fluid resuscitation. During resuscitation, a single dose of osthole (3 mg/kg, intravenously) with and without a p38 MAPK inhibitor SB-203580 (2 mg/kg, intravenously), SB-203580 or vehicle was administered. Plasma alanine aminotransferase (ALT) with aspartate aminotransferase (AST) concentrations and various hepatic parameters were measured (n = 8 rats/group) at 24 hours after resuscitation. The results showed that trauma-hemorrhage increased hepatic myeloperoxidase activity, intercellular adhesion molecule-1 and interleukin-6 levels, and plasma ALT and AST concentrations. These parameters were significantly improved in the osthole-treated rats subjected to trauma-hemorrhage. Osthole treatment also increased hepatic phospho-p38 MAPK expression compared with vehicle-treated trauma-hemorrhaged rats. Co-administration of SB-203580 with osthole abolished the osthole-induced beneficial effects on the above parameters and hepatic injury. These results suggest that the protective effect of osthole administration on alleviation of hepatic injury after trauma-hemorrhage, which is, at least in part, through p38 MAPK-dependent pathway. Public Library of Science 2013-06-06 /pmc/articles/PMC3675024/ /pubmed/23755293 http://dx.doi.org/10.1371/journal.pone.0065916 Text en © 2013 Yu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yu, Huang-Ping
Liu, Fu-Chao
Tsai, Yung-Fong
Hwang, Tsong-Long
Osthole Attenuates Hepatic Injury in a Rodent Model of Trauma-Hemorrhage
title Osthole Attenuates Hepatic Injury in a Rodent Model of Trauma-Hemorrhage
title_full Osthole Attenuates Hepatic Injury in a Rodent Model of Trauma-Hemorrhage
title_fullStr Osthole Attenuates Hepatic Injury in a Rodent Model of Trauma-Hemorrhage
title_full_unstemmed Osthole Attenuates Hepatic Injury in a Rodent Model of Trauma-Hemorrhage
title_short Osthole Attenuates Hepatic Injury in a Rodent Model of Trauma-Hemorrhage
title_sort osthole attenuates hepatic injury in a rodent model of trauma-hemorrhage
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675024/
https://www.ncbi.nlm.nih.gov/pubmed/23755293
http://dx.doi.org/10.1371/journal.pone.0065916
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