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CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver

CLEC4F, a member of C-type lectin, was first purified from rat liver extract with high binding affinity to fucose, galactose (Gal), N-acetylgalactosamine (GalNAc), and un-sialylated glucosphingolipids with GalNAc or Gal terminus. However, the biological functions of CLEC4F have not been elucidated....

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Autores principales: Yang, Chih-Ya, Chen, Jiun-Bo, Tsai, Ting-Fen, Tsai, Yi-Chen, Tsai, Ching-Yen, Liang, Pi-Hui, Hsu, Tsui-Ling, Wu, Chung-Yi, Netea, Mihai G., Wong, Chi-Huey, Hsieh, Shie-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675125/
https://www.ncbi.nlm.nih.gov/pubmed/23762286
http://dx.doi.org/10.1371/journal.pone.0065070
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author Yang, Chih-Ya
Chen, Jiun-Bo
Tsai, Ting-Fen
Tsai, Yi-Chen
Tsai, Ching-Yen
Liang, Pi-Hui
Hsu, Tsui-Ling
Wu, Chung-Yi
Netea, Mihai G.
Wong, Chi-Huey
Hsieh, Shie-Liang
author_facet Yang, Chih-Ya
Chen, Jiun-Bo
Tsai, Ting-Fen
Tsai, Yi-Chen
Tsai, Ching-Yen
Liang, Pi-Hui
Hsu, Tsui-Ling
Wu, Chung-Yi
Netea, Mihai G.
Wong, Chi-Huey
Hsieh, Shie-Liang
author_sort Yang, Chih-Ya
collection PubMed
description CLEC4F, a member of C-type lectin, was first purified from rat liver extract with high binding affinity to fucose, galactose (Gal), N-acetylgalactosamine (GalNAc), and un-sialylated glucosphingolipids with GalNAc or Gal terminus. However, the biological functions of CLEC4F have not been elucidated. To address this question, we examined the expression and distribution of murine CLEC4F, determined its binding specificity by glycan array, and investigated its function using CLEC4F knockout (Clec4f−/−) mice. We found that CLEC4F is a heavily glycosylated membrane protein co-expressed with F4/80 on Kupffer cells. In contrast to F4/80, CLEC4F is detectable in fetal livers at embryonic day 11.5 (E11.5) but not in yolk sac, suggesting the expression of CLEC4F is induced as cells migrate from yolk cells to the liver. Even though CLEC4F is not detectable in tissues outside liver, both residential Kupffer cells and infiltrating mononuclear cells surrounding liver abscesses are CLEC4F-positive upon Listeria monocytogenes (L. monocytogenes) infection. While CLEC4F has strong binding to Gal and GalNAc, terminal fucosylation inhibits CLEC4F recognition to several glycans such as Fucosyl GM1, Globo H, Bb3∼4 and other fucosyl-glycans. Moreover, CLEC4F interacts with alpha-galactosylceramide (α-GalCer) in a calcium-dependent manner and participates in the presentation of α-GalCer to natural killer T (NKT) cells. This suggests that CLEC4F is a C-type lectin with diverse binding specificity expressed on residential Kupffer cells and infiltrating monocytes in the liver, and may play an important role to modulate glycolipids presentation on Kupffer cells.
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spelling pubmed-36751252013-06-12 CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver Yang, Chih-Ya Chen, Jiun-Bo Tsai, Ting-Fen Tsai, Yi-Chen Tsai, Ching-Yen Liang, Pi-Hui Hsu, Tsui-Ling Wu, Chung-Yi Netea, Mihai G. Wong, Chi-Huey Hsieh, Shie-Liang PLoS One Research Article CLEC4F, a member of C-type lectin, was first purified from rat liver extract with high binding affinity to fucose, galactose (Gal), N-acetylgalactosamine (GalNAc), and un-sialylated glucosphingolipids with GalNAc or Gal terminus. However, the biological functions of CLEC4F have not been elucidated. To address this question, we examined the expression and distribution of murine CLEC4F, determined its binding specificity by glycan array, and investigated its function using CLEC4F knockout (Clec4f−/−) mice. We found that CLEC4F is a heavily glycosylated membrane protein co-expressed with F4/80 on Kupffer cells. In contrast to F4/80, CLEC4F is detectable in fetal livers at embryonic day 11.5 (E11.5) but not in yolk sac, suggesting the expression of CLEC4F is induced as cells migrate from yolk cells to the liver. Even though CLEC4F is not detectable in tissues outside liver, both residential Kupffer cells and infiltrating mononuclear cells surrounding liver abscesses are CLEC4F-positive upon Listeria monocytogenes (L. monocytogenes) infection. While CLEC4F has strong binding to Gal and GalNAc, terminal fucosylation inhibits CLEC4F recognition to several glycans such as Fucosyl GM1, Globo H, Bb3∼4 and other fucosyl-glycans. Moreover, CLEC4F interacts with alpha-galactosylceramide (α-GalCer) in a calcium-dependent manner and participates in the presentation of α-GalCer to natural killer T (NKT) cells. This suggests that CLEC4F is a C-type lectin with diverse binding specificity expressed on residential Kupffer cells and infiltrating monocytes in the liver, and may play an important role to modulate glycolipids presentation on Kupffer cells. Public Library of Science 2013-06-06 /pmc/articles/PMC3675125/ /pubmed/23762286 http://dx.doi.org/10.1371/journal.pone.0065070 Text en © 2013 Yang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yang, Chih-Ya
Chen, Jiun-Bo
Tsai, Ting-Fen
Tsai, Yi-Chen
Tsai, Ching-Yen
Liang, Pi-Hui
Hsu, Tsui-Ling
Wu, Chung-Yi
Netea, Mihai G.
Wong, Chi-Huey
Hsieh, Shie-Liang
CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver
title CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver
title_full CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver
title_fullStr CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver
title_full_unstemmed CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver
title_short CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver
title_sort clec4f is an inducible c-type lectin in f4/80-positive cells and is involved in alpha-galactosylceramide presentation in liver
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675125/
https://www.ncbi.nlm.nih.gov/pubmed/23762286
http://dx.doi.org/10.1371/journal.pone.0065070
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