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Electrochemical Determination of Low Molecular Mass Thiols Content in Potatoes (Solanum tuberosum) Cultivated in the Presence of Various Sulphur Forms and Infected by Late Blight (Phytophora infestans)

In the present paper potato plants were cultivated in the presence of ammonium sulphate or elemental sulphur supplementation into the soil to reveal the effects of different sulphur forms on content of nitrogen, phosphorus, potassium, calcium, magnesium and sulphur, and yield of tubers. During the i...

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Autores principales: Ryant, Pavel, Dolezelova, Eva, Fabrik, Ivo, Baloun, Jiri, Adam, Vojtech, Babula, Petr, Kizek, Rene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675538/
https://www.ncbi.nlm.nih.gov/pubmed/27879872
http://dx.doi.org/10.3390/s8053165
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author Ryant, Pavel
Dolezelova, Eva
Fabrik, Ivo
Baloun, Jiri
Adam, Vojtech
Babula, Petr
Kizek, Rene
author_facet Ryant, Pavel
Dolezelova, Eva
Fabrik, Ivo
Baloun, Jiri
Adam, Vojtech
Babula, Petr
Kizek, Rene
author_sort Ryant, Pavel
collection PubMed
description In the present paper potato plants were cultivated in the presence of ammonium sulphate or elemental sulphur supplementation into the soil to reveal the effects of different sulphur forms on content of nitrogen, phosphorus, potassium, calcium, magnesium and sulphur, and yield of tubers. During the investigation of the influence of different sulphur forms on yield of potato tubers we did not observe significant changes. Average weight of tubers of control plants per one experimental pot was 355 g. Application of sulphur in both forms resulted in moderate potato tubers weight reduction per one experimental pot compared to control group; average value ranged from 320 to 350 g per one experimental pot. Further we treated the plants with two different supplementation of sulphur with cadmium(II) ions (4 mg of cadmium(II) acetate per kilogram of the soil). The significantly lowest cadmium content (p < 0.05) was determined in tissues of plants treated with the highest dosage of elemental sulphur (0.64 mg Cd/kg) compared to control plants (0.82 mg Cd/kg). We also aimed our attention on the cadmium content in proteins, lipids or soluble carbohydrates and ash. Application of sulphate as well as elemental sulphur resulted in significant cadmium content reduction in lipid fraction compared to control plants. In addition to this we quantified content of low molecular mass thiols in potatoes tissues. To determine the thiols content we employed differential pulse voltammetry Brdicka reaction. After twelve days of the treatment enhancing of thiols level was observed in all experimental groups regardless to applied sulphur form and its concentration. Finally we evaluated the effect of sulphur supplementation on Phytophora infestans infection of potato plants.
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spelling pubmed-36755382013-06-19 Electrochemical Determination of Low Molecular Mass Thiols Content in Potatoes (Solanum tuberosum) Cultivated in the Presence of Various Sulphur Forms and Infected by Late Blight (Phytophora infestans) Ryant, Pavel Dolezelova, Eva Fabrik, Ivo Baloun, Jiri Adam, Vojtech Babula, Petr Kizek, Rene Sensors (Basel) Article In the present paper potato plants were cultivated in the presence of ammonium sulphate or elemental sulphur supplementation into the soil to reveal the effects of different sulphur forms on content of nitrogen, phosphorus, potassium, calcium, magnesium and sulphur, and yield of tubers. During the investigation of the influence of different sulphur forms on yield of potato tubers we did not observe significant changes. Average weight of tubers of control plants per one experimental pot was 355 g. Application of sulphur in both forms resulted in moderate potato tubers weight reduction per one experimental pot compared to control group; average value ranged from 320 to 350 g per one experimental pot. Further we treated the plants with two different supplementation of sulphur with cadmium(II) ions (4 mg of cadmium(II) acetate per kilogram of the soil). The significantly lowest cadmium content (p < 0.05) was determined in tissues of plants treated with the highest dosage of elemental sulphur (0.64 mg Cd/kg) compared to control plants (0.82 mg Cd/kg). We also aimed our attention on the cadmium content in proteins, lipids or soluble carbohydrates and ash. Application of sulphate as well as elemental sulphur resulted in significant cadmium content reduction in lipid fraction compared to control plants. In addition to this we quantified content of low molecular mass thiols in potatoes tissues. To determine the thiols content we employed differential pulse voltammetry Brdicka reaction. After twelve days of the treatment enhancing of thiols level was observed in all experimental groups regardless to applied sulphur form and its concentration. Finally we evaluated the effect of sulphur supplementation on Phytophora infestans infection of potato plants. Molecular Diversity Preservation International (MDPI) 2008-05-15 /pmc/articles/PMC3675538/ /pubmed/27879872 http://dx.doi.org/10.3390/s8053165 Text en © 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the CreativeCommons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Ryant, Pavel
Dolezelova, Eva
Fabrik, Ivo
Baloun, Jiri
Adam, Vojtech
Babula, Petr
Kizek, Rene
Electrochemical Determination of Low Molecular Mass Thiols Content in Potatoes (Solanum tuberosum) Cultivated in the Presence of Various Sulphur Forms and Infected by Late Blight (Phytophora infestans)
title Electrochemical Determination of Low Molecular Mass Thiols Content in Potatoes (Solanum tuberosum) Cultivated in the Presence of Various Sulphur Forms and Infected by Late Blight (Phytophora infestans)
title_full Electrochemical Determination of Low Molecular Mass Thiols Content in Potatoes (Solanum tuberosum) Cultivated in the Presence of Various Sulphur Forms and Infected by Late Blight (Phytophora infestans)
title_fullStr Electrochemical Determination of Low Molecular Mass Thiols Content in Potatoes (Solanum tuberosum) Cultivated in the Presence of Various Sulphur Forms and Infected by Late Blight (Phytophora infestans)
title_full_unstemmed Electrochemical Determination of Low Molecular Mass Thiols Content in Potatoes (Solanum tuberosum) Cultivated in the Presence of Various Sulphur Forms and Infected by Late Blight (Phytophora infestans)
title_short Electrochemical Determination of Low Molecular Mass Thiols Content in Potatoes (Solanum tuberosum) Cultivated in the Presence of Various Sulphur Forms and Infected by Late Blight (Phytophora infestans)
title_sort electrochemical determination of low molecular mass thiols content in potatoes (solanum tuberosum) cultivated in the presence of various sulphur forms and infected by late blight (phytophora infestans)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675538/
https://www.ncbi.nlm.nih.gov/pubmed/27879872
http://dx.doi.org/10.3390/s8053165
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