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Identification of a Novel Metalloproteinase and Its Role in Juvenile Development of the Tobacco Hornworm, Manduca sexta (Linnaeus)

Matrix metalloproteinases (MMPs) are a class of zinc-dependent endopeptidases that are highly conserved across numerous taxa, from bacteria to humans. Recently, MMPs have been identified in several insect species and are hypothesized to function in immunity and development. In this study, we identif...

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Autores principales: Vishnuvardhan, Smitha, Ahsan, Rubina, Jackson, Kathryn, Iwanicki, Rebecca, Boe, Jordan, Haring, Jodie, Greenlee, Kendra J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675644/
https://www.ncbi.nlm.nih.gov/pubmed/23475557
http://dx.doi.org/10.1002/jez.b.22487
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author Vishnuvardhan, Smitha
Ahsan, Rubina
Jackson, Kathryn
Iwanicki, Rebecca
Boe, Jordan
Haring, Jodie
Greenlee, Kendra J
author_facet Vishnuvardhan, Smitha
Ahsan, Rubina
Jackson, Kathryn
Iwanicki, Rebecca
Boe, Jordan
Haring, Jodie
Greenlee, Kendra J
author_sort Vishnuvardhan, Smitha
collection PubMed
description Matrix metalloproteinases (MMPs) are a class of zinc-dependent endopeptidases that are highly conserved across numerous taxa, from bacteria to humans. Recently, MMPs have been identified in several insect species and are hypothesized to function in immunity and development. In this study, we identify a putative MMP and correlate its proteolytic activity and gene and protein expression in the tracheae with developmental stage. Ms-MMP gene expression increases 10-fold during molting, which is accompanied by an increase in both protein expression and gelatinolytic activity. To directly test the hypothesis that Ms-MMP plays a critical role in juvenile development of Manduca sexta, we injected a broad-spectrum MMP inhibitor and recorded its effects on growth and development. Inhibition of MMPs caused a delay in juvenile development and decreased growth rates. Understanding the function of MMPs will help us better understand molting and control of body size in insects. Furthermore, elucidating functions for MMPs in lower taxa may yield critical information about the evolution of the numerous MMPs found in vertebrates. J. Exp. Zool. (Mol. Dev. Evol.) 320B:105–117, 2013. © 2013 Wiley Periodicals, Inc.
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spelling pubmed-36756442013-06-10 Identification of a Novel Metalloproteinase and Its Role in Juvenile Development of the Tobacco Hornworm, Manduca sexta (Linnaeus) Vishnuvardhan, Smitha Ahsan, Rubina Jackson, Kathryn Iwanicki, Rebecca Boe, Jordan Haring, Jodie Greenlee, Kendra J J Exp Zool B Mol Dev Evol Research Articles Matrix metalloproteinases (MMPs) are a class of zinc-dependent endopeptidases that are highly conserved across numerous taxa, from bacteria to humans. Recently, MMPs have been identified in several insect species and are hypothesized to function in immunity and development. In this study, we identify a putative MMP and correlate its proteolytic activity and gene and protein expression in the tracheae with developmental stage. Ms-MMP gene expression increases 10-fold during molting, which is accompanied by an increase in both protein expression and gelatinolytic activity. To directly test the hypothesis that Ms-MMP plays a critical role in juvenile development of Manduca sexta, we injected a broad-spectrum MMP inhibitor and recorded its effects on growth and development. Inhibition of MMPs caused a delay in juvenile development and decreased growth rates. Understanding the function of MMPs will help us better understand molting and control of body size in insects. Furthermore, elucidating functions for MMPs in lower taxa may yield critical information about the evolution of the numerous MMPs found in vertebrates. J. Exp. Zool. (Mol. Dev. Evol.) 320B:105–117, 2013. © 2013 Wiley Periodicals, Inc. Blackwell Publishing Ltd 2013-03 2013-01-25 /pmc/articles/PMC3675644/ /pubmed/23475557 http://dx.doi.org/10.1002/jez.b.22487 Text en Copyright © 2013 Wiley Periodicals, Inc. http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Research Articles
Vishnuvardhan, Smitha
Ahsan, Rubina
Jackson, Kathryn
Iwanicki, Rebecca
Boe, Jordan
Haring, Jodie
Greenlee, Kendra J
Identification of a Novel Metalloproteinase and Its Role in Juvenile Development of the Tobacco Hornworm, Manduca sexta (Linnaeus)
title Identification of a Novel Metalloproteinase and Its Role in Juvenile Development of the Tobacco Hornworm, Manduca sexta (Linnaeus)
title_full Identification of a Novel Metalloproteinase and Its Role in Juvenile Development of the Tobacco Hornworm, Manduca sexta (Linnaeus)
title_fullStr Identification of a Novel Metalloproteinase and Its Role in Juvenile Development of the Tobacco Hornworm, Manduca sexta (Linnaeus)
title_full_unstemmed Identification of a Novel Metalloproteinase and Its Role in Juvenile Development of the Tobacco Hornworm, Manduca sexta (Linnaeus)
title_short Identification of a Novel Metalloproteinase and Its Role in Juvenile Development of the Tobacco Hornworm, Manduca sexta (Linnaeus)
title_sort identification of a novel metalloproteinase and its role in juvenile development of the tobacco hornworm, manduca sexta (linnaeus)
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675644/
https://www.ncbi.nlm.nih.gov/pubmed/23475557
http://dx.doi.org/10.1002/jez.b.22487
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