QuaNCAT: quantitating proteome dynamics in primary cells

Here we demonstrate that quantitation of stimuli-induced proteome dynamics in primary cells is feasible by combining the power of Bio-Orthogonal Non Canonical Amino acid Tagging (BONCAT) and Stable Isotope Labelling of Amino acids in Cell culture (SILAC). In conjunction with nanoLC-MS/MS QuaNCAT all...

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Autores principales: Howden, Andrew J.M., Geoghegan, Vincent, Katsch, Kristin, Efstathiou, Georgios, Bhushan, Bhaskar, Boutureira, Omar, Thomas, Benjamin, Trudgian, David C., Kessler, Benedikt M., Dieterich, Daniela C., Davis, Benjamin G., Acuto, Oreste
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676679/
https://www.ncbi.nlm.nih.gov/pubmed/23474466
http://dx.doi.org/10.1038/nmeth.2401
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author Howden, Andrew J.M.
Geoghegan, Vincent
Katsch, Kristin
Efstathiou, Georgios
Bhushan, Bhaskar
Boutureira, Omar
Thomas, Benjamin
Trudgian, David C.
Kessler, Benedikt M.
Dieterich, Daniela C.
Davis, Benjamin G.
Acuto, Oreste
author_facet Howden, Andrew J.M.
Geoghegan, Vincent
Katsch, Kristin
Efstathiou, Georgios
Bhushan, Bhaskar
Boutureira, Omar
Thomas, Benjamin
Trudgian, David C.
Kessler, Benedikt M.
Dieterich, Daniela C.
Davis, Benjamin G.
Acuto, Oreste
author_sort Howden, Andrew J.M.
collection PubMed
description Here we demonstrate that quantitation of stimuli-induced proteome dynamics in primary cells is feasible by combining the power of Bio-Orthogonal Non Canonical Amino acid Tagging (BONCAT) and Stable Isotope Labelling of Amino acids in Cell culture (SILAC). In conjunction with nanoLC-MS/MS QuaNCAT allowed us to monitor the early expression changes of > 600 proteins in primary resting T cells subjected to activation stimuli.
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spelling pubmed-36766792013-10-01 QuaNCAT: quantitating proteome dynamics in primary cells Howden, Andrew J.M. Geoghegan, Vincent Katsch, Kristin Efstathiou, Georgios Bhushan, Bhaskar Boutureira, Omar Thomas, Benjamin Trudgian, David C. Kessler, Benedikt M. Dieterich, Daniela C. Davis, Benjamin G. Acuto, Oreste Nat Methods Article Here we demonstrate that quantitation of stimuli-induced proteome dynamics in primary cells is feasible by combining the power of Bio-Orthogonal Non Canonical Amino acid Tagging (BONCAT) and Stable Isotope Labelling of Amino acids in Cell culture (SILAC). In conjunction with nanoLC-MS/MS QuaNCAT allowed us to monitor the early expression changes of > 600 proteins in primary resting T cells subjected to activation stimuli. 2013-03-10 2013-04 /pmc/articles/PMC3676679/ /pubmed/23474466 http://dx.doi.org/10.1038/nmeth.2401 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Howden, Andrew J.M.
Geoghegan, Vincent
Katsch, Kristin
Efstathiou, Georgios
Bhushan, Bhaskar
Boutureira, Omar
Thomas, Benjamin
Trudgian, David C.
Kessler, Benedikt M.
Dieterich, Daniela C.
Davis, Benjamin G.
Acuto, Oreste
QuaNCAT: quantitating proteome dynamics in primary cells
title QuaNCAT: quantitating proteome dynamics in primary cells
title_full QuaNCAT: quantitating proteome dynamics in primary cells
title_fullStr QuaNCAT: quantitating proteome dynamics in primary cells
title_full_unstemmed QuaNCAT: quantitating proteome dynamics in primary cells
title_short QuaNCAT: quantitating proteome dynamics in primary cells
title_sort quancat: quantitating proteome dynamics in primary cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676679/
https://www.ncbi.nlm.nih.gov/pubmed/23474466
http://dx.doi.org/10.1038/nmeth.2401
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