Cargando…
Differential Activity of Plasma and Vacuolar Membrane Transporters Contributes to Genotypic Differences in Salinity Tolerance in a Halophyte Species, Chenopodium quinoa
Halophytes species can be used as a highly convenient model system to reveal key ionic and molecular mechanisms that confer salinity tolerance in plants. Earlier, we reported that quinoa (Chenopodium quinoa Willd.), a facultative C3 halophyte species, can efficiently control the activity of slow (SV...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676782/ https://www.ncbi.nlm.nih.gov/pubmed/23629664 http://dx.doi.org/10.3390/ijms14059267 |
_version_ | 1782272664544477184 |
---|---|
author | Bonales-Alatorre, Edgar Pottosin, Igor Shabala, Lana Chen, Zhong-Hua Zeng, Fanrong Jacobsen, Sven-Erik Shabala, Sergey |
author_facet | Bonales-Alatorre, Edgar Pottosin, Igor Shabala, Lana Chen, Zhong-Hua Zeng, Fanrong Jacobsen, Sven-Erik Shabala, Sergey |
author_sort | Bonales-Alatorre, Edgar |
collection | PubMed |
description | Halophytes species can be used as a highly convenient model system to reveal key ionic and molecular mechanisms that confer salinity tolerance in plants. Earlier, we reported that quinoa (Chenopodium quinoa Willd.), a facultative C3 halophyte species, can efficiently control the activity of slow (SV) and fast (FV) tonoplast channels to match specific growth conditions by ensuring that most of accumulated Na(+) is safely locked in the vacuole (Bonales-Alatorre et al. (2013) Plant Physiology). This work extends these finding by comparing the properties of tonoplast FV and SV channels in two quinoa genotypes contrasting in their salinity tolerance. The work is complemented by studies of the kinetics of net ion fluxes across the plasma membrane of quinoa leaf mesophyll tissue. Our results suggest that multiple mechanisms contribute towards genotypic differences in salinity tolerance in quinoa. These include: (i) a higher rate of Na(+) exclusion from leaf mesophyll; (ii) maintenance of low cytosolic Na(+) levels; (iii) better K(+) retention in the leaf mesophyll; (iv) a high rate of H(+) pumping, which increases the ability of mesophyll cells to restore their membrane potential; and (v) the ability to reduce the activity of SV and FV channels under saline conditions. These mechanisms appear to be highly orchestrated, thus enabling the remarkable overall salinity tolerance of quinoa species. |
format | Online Article Text |
id | pubmed-3676782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-36767822013-07-02 Differential Activity of Plasma and Vacuolar Membrane Transporters Contributes to Genotypic Differences in Salinity Tolerance in a Halophyte Species, Chenopodium quinoa Bonales-Alatorre, Edgar Pottosin, Igor Shabala, Lana Chen, Zhong-Hua Zeng, Fanrong Jacobsen, Sven-Erik Shabala, Sergey Int J Mol Sci Article Halophytes species can be used as a highly convenient model system to reveal key ionic and molecular mechanisms that confer salinity tolerance in plants. Earlier, we reported that quinoa (Chenopodium quinoa Willd.), a facultative C3 halophyte species, can efficiently control the activity of slow (SV) and fast (FV) tonoplast channels to match specific growth conditions by ensuring that most of accumulated Na(+) is safely locked in the vacuole (Bonales-Alatorre et al. (2013) Plant Physiology). This work extends these finding by comparing the properties of tonoplast FV and SV channels in two quinoa genotypes contrasting in their salinity tolerance. The work is complemented by studies of the kinetics of net ion fluxes across the plasma membrane of quinoa leaf mesophyll tissue. Our results suggest that multiple mechanisms contribute towards genotypic differences in salinity tolerance in quinoa. These include: (i) a higher rate of Na(+) exclusion from leaf mesophyll; (ii) maintenance of low cytosolic Na(+) levels; (iii) better K(+) retention in the leaf mesophyll; (iv) a high rate of H(+) pumping, which increases the ability of mesophyll cells to restore their membrane potential; and (v) the ability to reduce the activity of SV and FV channels under saline conditions. These mechanisms appear to be highly orchestrated, thus enabling the remarkable overall salinity tolerance of quinoa species. Molecular Diversity Preservation International (MDPI) 2013-04-29 /pmc/articles/PMC3676782/ /pubmed/23629664 http://dx.doi.org/10.3390/ijms14059267 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Bonales-Alatorre, Edgar Pottosin, Igor Shabala, Lana Chen, Zhong-Hua Zeng, Fanrong Jacobsen, Sven-Erik Shabala, Sergey Differential Activity of Plasma and Vacuolar Membrane Transporters Contributes to Genotypic Differences in Salinity Tolerance in a Halophyte Species, Chenopodium quinoa |
title | Differential Activity of Plasma and Vacuolar Membrane Transporters Contributes to Genotypic Differences in Salinity Tolerance in a Halophyte Species, Chenopodium quinoa |
title_full | Differential Activity of Plasma and Vacuolar Membrane Transporters Contributes to Genotypic Differences in Salinity Tolerance in a Halophyte Species, Chenopodium quinoa |
title_fullStr | Differential Activity of Plasma and Vacuolar Membrane Transporters Contributes to Genotypic Differences in Salinity Tolerance in a Halophyte Species, Chenopodium quinoa |
title_full_unstemmed | Differential Activity of Plasma and Vacuolar Membrane Transporters Contributes to Genotypic Differences in Salinity Tolerance in a Halophyte Species, Chenopodium quinoa |
title_short | Differential Activity of Plasma and Vacuolar Membrane Transporters Contributes to Genotypic Differences in Salinity Tolerance in a Halophyte Species, Chenopodium quinoa |
title_sort | differential activity of plasma and vacuolar membrane transporters contributes to genotypic differences in salinity tolerance in a halophyte species, chenopodium quinoa |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676782/ https://www.ncbi.nlm.nih.gov/pubmed/23629664 http://dx.doi.org/10.3390/ijms14059267 |
work_keys_str_mv | AT bonalesalatorreedgar differentialactivityofplasmaandvacuolarmembranetransporterscontributestogenotypicdifferencesinsalinitytoleranceinahalophytespecieschenopodiumquinoa AT pottosinigor differentialactivityofplasmaandvacuolarmembranetransporterscontributestogenotypicdifferencesinsalinitytoleranceinahalophytespecieschenopodiumquinoa AT shabalalana differentialactivityofplasmaandvacuolarmembranetransporterscontributestogenotypicdifferencesinsalinitytoleranceinahalophytespecieschenopodiumquinoa AT chenzhonghua differentialactivityofplasmaandvacuolarmembranetransporterscontributestogenotypicdifferencesinsalinitytoleranceinahalophytespecieschenopodiumquinoa AT zengfanrong differentialactivityofplasmaandvacuolarmembranetransporterscontributestogenotypicdifferencesinsalinitytoleranceinahalophytespecieschenopodiumquinoa AT jacobsensvenerik differentialactivityofplasmaandvacuolarmembranetransporterscontributestogenotypicdifferencesinsalinitytoleranceinahalophytespecieschenopodiumquinoa AT shabalasergey differentialactivityofplasmaandvacuolarmembranetransporterscontributestogenotypicdifferencesinsalinitytoleranceinahalophytespecieschenopodiumquinoa |