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Characterization of Rice NADPH Oxidase Genes and Their Expression under Various Environmental Conditions

Plasma membrane NADPH oxidases (Noxs) are key producers of reactive oxygen species under both normal and stress conditions in plants. We demonstrate that at least eleven genes in the genome of rice (Oryza sativa L.) were predicted to encode Nox proteins, including nine genes (OsNox1–9) that encode t...

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Autores principales: Wang, Gang-Feng, Li, Wen-Qiang, Li, Wen-Yan, Wu, Guo-Li, Zhou, Cong-Yi, Chen, Kun-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676792/
https://www.ncbi.nlm.nih.gov/pubmed/23629674
http://dx.doi.org/10.3390/ijms14059440
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author Wang, Gang-Feng
Li, Wen-Qiang
Li, Wen-Yan
Wu, Guo-Li
Zhou, Cong-Yi
Chen, Kun-Ming
author_facet Wang, Gang-Feng
Li, Wen-Qiang
Li, Wen-Yan
Wu, Guo-Li
Zhou, Cong-Yi
Chen, Kun-Ming
author_sort Wang, Gang-Feng
collection PubMed
description Plasma membrane NADPH oxidases (Noxs) are key producers of reactive oxygen species under both normal and stress conditions in plants. We demonstrate that at least eleven genes in the genome of rice (Oryza sativa L.) were predicted to encode Nox proteins, including nine genes (OsNox1–9) that encode typical Noxs and two that encode ancient Nox forms (ferric reduction oxidase 1 and 7, OsFRO1 and OsFRO7). Phylogenetic analysis divided the Noxs from nine plant species into six subfamilies, with rice Nox genes distributed among subfamilies I to V. Gene expression analysis using semi-quantitative RT-PCR and real-time qRT-PCR indicated that the expression of rice Nox genes depends on organs and environmental conditions. Exogenous calcium strongly stimulated the expression of OsNox3, OsNox5, OsNox7, and OsNox8, but depressed the expression of OsFRO1. Drought stress substantially upregulated the expression of OsNox1–3, OsNox5, OsNox9, and OsFRO1, but downregulated OsNox6. High temperature upregulated OsNox5–9, but significantly downregulated OsNox1–3 and OsFRO1. NaCl treatment increased the expression of OsNox2, OsNox8, OsFRO1, and OsFRO7, but decreased that of OsNox1, OsNox3, OsNox5, and OsNox6. These results suggest that the expression profiles of rice Nox genes have unique stress-response characteristics, reflecting their related but distinct functions in response to different environmental stresses.
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spelling pubmed-36767922013-07-02 Characterization of Rice NADPH Oxidase Genes and Their Expression under Various Environmental Conditions Wang, Gang-Feng Li, Wen-Qiang Li, Wen-Yan Wu, Guo-Li Zhou, Cong-Yi Chen, Kun-Ming Int J Mol Sci Article Plasma membrane NADPH oxidases (Noxs) are key producers of reactive oxygen species under both normal and stress conditions in plants. We demonstrate that at least eleven genes in the genome of rice (Oryza sativa L.) were predicted to encode Nox proteins, including nine genes (OsNox1–9) that encode typical Noxs and two that encode ancient Nox forms (ferric reduction oxidase 1 and 7, OsFRO1 and OsFRO7). Phylogenetic analysis divided the Noxs from nine plant species into six subfamilies, with rice Nox genes distributed among subfamilies I to V. Gene expression analysis using semi-quantitative RT-PCR and real-time qRT-PCR indicated that the expression of rice Nox genes depends on organs and environmental conditions. Exogenous calcium strongly stimulated the expression of OsNox3, OsNox5, OsNox7, and OsNox8, but depressed the expression of OsFRO1. Drought stress substantially upregulated the expression of OsNox1–3, OsNox5, OsNox9, and OsFRO1, but downregulated OsNox6. High temperature upregulated OsNox5–9, but significantly downregulated OsNox1–3 and OsFRO1. NaCl treatment increased the expression of OsNox2, OsNox8, OsFRO1, and OsFRO7, but decreased that of OsNox1, OsNox3, OsNox5, and OsNox6. These results suggest that the expression profiles of rice Nox genes have unique stress-response characteristics, reflecting their related but distinct functions in response to different environmental stresses. Molecular Diversity Preservation International (MDPI) 2013-04-29 /pmc/articles/PMC3676792/ /pubmed/23629674 http://dx.doi.org/10.3390/ijms14059440 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wang, Gang-Feng
Li, Wen-Qiang
Li, Wen-Yan
Wu, Guo-Li
Zhou, Cong-Yi
Chen, Kun-Ming
Characterization of Rice NADPH Oxidase Genes and Their Expression under Various Environmental Conditions
title Characterization of Rice NADPH Oxidase Genes and Their Expression under Various Environmental Conditions
title_full Characterization of Rice NADPH Oxidase Genes and Their Expression under Various Environmental Conditions
title_fullStr Characterization of Rice NADPH Oxidase Genes and Their Expression under Various Environmental Conditions
title_full_unstemmed Characterization of Rice NADPH Oxidase Genes and Their Expression under Various Environmental Conditions
title_short Characterization of Rice NADPH Oxidase Genes and Their Expression under Various Environmental Conditions
title_sort characterization of rice nadph oxidase genes and their expression under various environmental conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676792/
https://www.ncbi.nlm.nih.gov/pubmed/23629674
http://dx.doi.org/10.3390/ijms14059440
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