Cargando…
Resveratrol Inhibits Alpha-Melanocyte-Stimulating Hormone Signaling, Viability, and Invasiveness in Melanoma Cells
Melanoma is a malignancy with high potential to invasion and treatment resistance. The α-melanocyte-stimulating hormone (α-MSH) signal transduction involving Wnt/β-catenin, c-Kit, and microphthalmia-associated transcription factor (MITF), a known pathway to produce melanin, has been demonstrated as...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676971/ https://www.ncbi.nlm.nih.gov/pubmed/23762150 http://dx.doi.org/10.1155/2013/632121 |
_version_ | 1782272690321620992 |
---|---|
author | Chen, Yu-Jen Chen, Ying-Yin Lin, Yi-Feng Hu, Hsuan-Yun Liao, Hui-Fen |
author_facet | Chen, Yu-Jen Chen, Ying-Yin Lin, Yi-Feng Hu, Hsuan-Yun Liao, Hui-Fen |
author_sort | Chen, Yu-Jen |
collection | PubMed |
description | Melanoma is a malignancy with high potential to invasion and treatment resistance. The α-melanocyte-stimulating hormone (α-MSH) signal transduction involving Wnt/β-catenin, c-Kit, and microphthalmia-associated transcription factor (MITF), a known pathway to produce melanin, has been demonstrated as one of cancer stem cell characteristics. This study was aimed to examine the effect of resveratrol, an abundant ingredient of grape and medicinal plants, on α-MSH signaling, viability, and invasiveness in melanoma cells. By α-MSH treatment, the melanin production in B16 melanoma cells was augmented as a validation for activation of α-MSH signaling. The upregulated expression of α-MSH signaling-related molecules β-catenin, c-Kit, and MITF was suppressed by resveratrol and/or STI571 treatment. Nuclear translocation of MITF, a hallmark of α-MSH signaling activation, was inhibited by combined treatment of resveratrol and STI571. At effective concentration, resveratrol and/or STI571 inhibited cell viability and α-MSH-activated matrix metalloproteinase- (MMP-)9 expression and invasion capacity of B16 melanoma cells. In conclusion, resveratrol enhances STI571 effect on suppressing the α-MSH signaling, viability, and invasiveness in melanoma cells. It implicates that resveratrol may have potential to modulate the cancer stem cell characteristics of melanoma. |
format | Online Article Text |
id | pubmed-3676971 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36769712013-06-12 Resveratrol Inhibits Alpha-Melanocyte-Stimulating Hormone Signaling, Viability, and Invasiveness in Melanoma Cells Chen, Yu-Jen Chen, Ying-Yin Lin, Yi-Feng Hu, Hsuan-Yun Liao, Hui-Fen Evid Based Complement Alternat Med Research Article Melanoma is a malignancy with high potential to invasion and treatment resistance. The α-melanocyte-stimulating hormone (α-MSH) signal transduction involving Wnt/β-catenin, c-Kit, and microphthalmia-associated transcription factor (MITF), a known pathway to produce melanin, has been demonstrated as one of cancer stem cell characteristics. This study was aimed to examine the effect of resveratrol, an abundant ingredient of grape and medicinal plants, on α-MSH signaling, viability, and invasiveness in melanoma cells. By α-MSH treatment, the melanin production in B16 melanoma cells was augmented as a validation for activation of α-MSH signaling. The upregulated expression of α-MSH signaling-related molecules β-catenin, c-Kit, and MITF was suppressed by resveratrol and/or STI571 treatment. Nuclear translocation of MITF, a hallmark of α-MSH signaling activation, was inhibited by combined treatment of resveratrol and STI571. At effective concentration, resveratrol and/or STI571 inhibited cell viability and α-MSH-activated matrix metalloproteinase- (MMP-)9 expression and invasion capacity of B16 melanoma cells. In conclusion, resveratrol enhances STI571 effect on suppressing the α-MSH signaling, viability, and invasiveness in melanoma cells. It implicates that resveratrol may have potential to modulate the cancer stem cell characteristics of melanoma. Hindawi Publishing Corporation 2013 2013-05-23 /pmc/articles/PMC3676971/ /pubmed/23762150 http://dx.doi.org/10.1155/2013/632121 Text en Copyright © 2013 Yu-Jen Chen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chen, Yu-Jen Chen, Ying-Yin Lin, Yi-Feng Hu, Hsuan-Yun Liao, Hui-Fen Resveratrol Inhibits Alpha-Melanocyte-Stimulating Hormone Signaling, Viability, and Invasiveness in Melanoma Cells |
title | Resveratrol Inhibits Alpha-Melanocyte-Stimulating Hormone Signaling, Viability, and Invasiveness in Melanoma Cells |
title_full | Resveratrol Inhibits Alpha-Melanocyte-Stimulating Hormone Signaling, Viability, and Invasiveness in Melanoma Cells |
title_fullStr | Resveratrol Inhibits Alpha-Melanocyte-Stimulating Hormone Signaling, Viability, and Invasiveness in Melanoma Cells |
title_full_unstemmed | Resveratrol Inhibits Alpha-Melanocyte-Stimulating Hormone Signaling, Viability, and Invasiveness in Melanoma Cells |
title_short | Resveratrol Inhibits Alpha-Melanocyte-Stimulating Hormone Signaling, Viability, and Invasiveness in Melanoma Cells |
title_sort | resveratrol inhibits alpha-melanocyte-stimulating hormone signaling, viability, and invasiveness in melanoma cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676971/ https://www.ncbi.nlm.nih.gov/pubmed/23762150 http://dx.doi.org/10.1155/2013/632121 |
work_keys_str_mv | AT chenyujen resveratrolinhibitsalphamelanocytestimulatinghormonesignalingviabilityandinvasivenessinmelanomacells AT chenyingyin resveratrolinhibitsalphamelanocytestimulatinghormonesignalingviabilityandinvasivenessinmelanomacells AT linyifeng resveratrolinhibitsalphamelanocytestimulatinghormonesignalingviabilityandinvasivenessinmelanomacells AT huhsuanyun resveratrolinhibitsalphamelanocytestimulatinghormonesignalingviabilityandinvasivenessinmelanomacells AT liaohuifen resveratrolinhibitsalphamelanocytestimulatinghormonesignalingviabilityandinvasivenessinmelanomacells |