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Recovery of DNA of Giardia intestinalis cysts from surface water concentrates measured with PCR and real time PCR

The most important restriction for the detection in water samples is the low concentration of Giardia intestinalis cysts, additional difficulty is the presence of PCR inhibitors. We have carried out trials in order to assess the sensitivity of semi-nested PCR and TaqMan real time PCR on the basis of...

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Detalles Bibliográficos
Autores principales: Adamska, M., Leońska-Duniec, A., Maciejewska, A., Sawczuk, M., Skotarczak, B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3677587/
https://www.ncbi.nlm.nih.gov/pubmed/22091466
http://dx.doi.org/10.1051/parasite/2011184341