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Tetrandrine Suppresses Cancer Angiogenesis and Metastasis in 4T1 Tumor Bearing Mice

Metastasis remains the most deadly aspect of cancer and still evades direct treatment. Thus, there is a great need to develop new treatment regimens to suppress tumor cells that have escaped surgical removal or that may have already disseminated. We have found that tetrandrine (TET) exhibits anticol...

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Autores principales: Gao, Jian-Li, Ji, Xing, He, Tong-Chuan, Zhang, Qi, He, Kai, Zhao, Yan, Chen, Su-Hong, Lv, Gui-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3677646/
https://www.ncbi.nlm.nih.gov/pubmed/23762115
http://dx.doi.org/10.1155/2013/265061
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author Gao, Jian-Li
Ji, Xing
He, Tong-Chuan
Zhang, Qi
He, Kai
Zhao, Yan
Chen, Su-Hong
Lv, Gui-Yuan
author_facet Gao, Jian-Li
Ji, Xing
He, Tong-Chuan
Zhang, Qi
He, Kai
Zhao, Yan
Chen, Su-Hong
Lv, Gui-Yuan
author_sort Gao, Jian-Li
collection PubMed
description Metastasis remains the most deadly aspect of cancer and still evades direct treatment. Thus, there is a great need to develop new treatment regimens to suppress tumor cells that have escaped surgical removal or that may have already disseminated. We have found that tetrandrine (TET) exhibits anticolon cancer activity. Here, we investigate the inhibition effect of TET to breast cancer metastasis, angiogenesis and its molecular basis underlying TET's anticancer activity. We compare TET with chemotherapy drug doxorubicin in 4T1 tumor bearing BALB/c mice model and find that TET exhibits an anticancer metastatic and antiangiogenic activities better than those of doxorubicin. The lung metastatic sites were decreased by TET, which is confirmed by bioluminescence imaging in vivo. On the other hand, laser doppler perfusion imaging (LDI) was used for measuring the blood flow of tumor in 4T1-tumor bearing mice. As a result, the local blood perfusion of tumor was markedly decreased by TET after 3 weeks. Mechanistically, TET treatment leads to a decrease in p-ERK level and an increase in NF-κB levels in HUVECs. TET also regulated metastatic and angiogenic related proteins, including vascular endothelial growth factor, hypoxia-inducible factor-1α, integrin β5, endothelial cell specific molecule-1, and intercellular adhesion molecule-1 in vivo.
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spelling pubmed-36776462013-06-12 Tetrandrine Suppresses Cancer Angiogenesis and Metastasis in 4T1 Tumor Bearing Mice Gao, Jian-Li Ji, Xing He, Tong-Chuan Zhang, Qi He, Kai Zhao, Yan Chen, Su-Hong Lv, Gui-Yuan Evid Based Complement Alternat Med Research Article Metastasis remains the most deadly aspect of cancer and still evades direct treatment. Thus, there is a great need to develop new treatment regimens to suppress tumor cells that have escaped surgical removal or that may have already disseminated. We have found that tetrandrine (TET) exhibits anticolon cancer activity. Here, we investigate the inhibition effect of TET to breast cancer metastasis, angiogenesis and its molecular basis underlying TET's anticancer activity. We compare TET with chemotherapy drug doxorubicin in 4T1 tumor bearing BALB/c mice model and find that TET exhibits an anticancer metastatic and antiangiogenic activities better than those of doxorubicin. The lung metastatic sites were decreased by TET, which is confirmed by bioluminescence imaging in vivo. On the other hand, laser doppler perfusion imaging (LDI) was used for measuring the blood flow of tumor in 4T1-tumor bearing mice. As a result, the local blood perfusion of tumor was markedly decreased by TET after 3 weeks. Mechanistically, TET treatment leads to a decrease in p-ERK level and an increase in NF-κB levels in HUVECs. TET also regulated metastatic and angiogenic related proteins, including vascular endothelial growth factor, hypoxia-inducible factor-1α, integrin β5, endothelial cell specific molecule-1, and intercellular adhesion molecule-1 in vivo. Hindawi Publishing Corporation 2013 2013-05-25 /pmc/articles/PMC3677646/ /pubmed/23762115 http://dx.doi.org/10.1155/2013/265061 Text en Copyright © 2013 Jian-Li Gao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gao, Jian-Li
Ji, Xing
He, Tong-Chuan
Zhang, Qi
He, Kai
Zhao, Yan
Chen, Su-Hong
Lv, Gui-Yuan
Tetrandrine Suppresses Cancer Angiogenesis and Metastasis in 4T1 Tumor Bearing Mice
title Tetrandrine Suppresses Cancer Angiogenesis and Metastasis in 4T1 Tumor Bearing Mice
title_full Tetrandrine Suppresses Cancer Angiogenesis and Metastasis in 4T1 Tumor Bearing Mice
title_fullStr Tetrandrine Suppresses Cancer Angiogenesis and Metastasis in 4T1 Tumor Bearing Mice
title_full_unstemmed Tetrandrine Suppresses Cancer Angiogenesis and Metastasis in 4T1 Tumor Bearing Mice
title_short Tetrandrine Suppresses Cancer Angiogenesis and Metastasis in 4T1 Tumor Bearing Mice
title_sort tetrandrine suppresses cancer angiogenesis and metastasis in 4t1 tumor bearing mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3677646/
https://www.ncbi.nlm.nih.gov/pubmed/23762115
http://dx.doi.org/10.1155/2013/265061
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