Cargando…
The Development of 7E Chromosome-Specific Molecular Markers for Thinopyrum elongatum Based on SLAF-seq Technology
Thinopyrum elongatum is an important relative of wheat, it is favored by many researchers for the disease resistant genes that exist in its E genome. Some studies have showed that the 7E chromosome of Th. elongatum contains resistance genes related to Fusarium head blight and wheat rust. Therefore,...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3677899/ https://www.ncbi.nlm.nih.gov/pubmed/23762296 http://dx.doi.org/10.1371/journal.pone.0065122 |
_version_ | 1782272773864816640 |
---|---|
author | Chen, Shiqiang Huang, Zefeng Dai, Yi Qin, Shuwen Gao, Yingying Zhang, Lulu Gao, Yong Chen, Jianmin |
author_facet | Chen, Shiqiang Huang, Zefeng Dai, Yi Qin, Shuwen Gao, Yingying Zhang, Lulu Gao, Yong Chen, Jianmin |
author_sort | Chen, Shiqiang |
collection | PubMed |
description | Thinopyrum elongatum is an important relative of wheat, it is favored by many researchers for the disease resistant genes that exist in its E genome. Some studies have showed that the 7E chromosome of Th. elongatum contains resistance genes related to Fusarium head blight and wheat rust. Therefore, developing 7E chromosome-specific molecular markers linked to resistance genes will provide an important tool for exploring and using the resistant genes of Th. elongatum. In addition, it would greatly contribute in the effort to cultivate disease-resistant wheat varieties. Featured in high throughput, high-accuracy and low-cost, SLAF-seq technology has been widely used in molecular breeding, system evolution, and germplasm resource detection. Based on SLAF-seq, 518 specific fragments on the 7E chromosome of Th. elongatum were successfully amplified. A total of 135 primers were designed according to 135 randomly selected fragments, and 89 specific molecular markers of Th. elongatum were developed, with efficiencies up to 65.9%. These markers were all detected in a variety of materials, and they are all proved to be specific and stable. These markers can be used not only for detecting the 7E chromosome of Th. elongatum but also for providing an important theoretical and practical basis for wheat breeding by marker-assisted selection (MAS). This paper reports the first application of SLAF-seq technology with a high success rate in developing specific molecular markers for Th. elongatum, providing a strong case for the application of this new technology. |
format | Online Article Text |
id | pubmed-3677899 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36778992013-06-12 The Development of 7E Chromosome-Specific Molecular Markers for Thinopyrum elongatum Based on SLAF-seq Technology Chen, Shiqiang Huang, Zefeng Dai, Yi Qin, Shuwen Gao, Yingying Zhang, Lulu Gao, Yong Chen, Jianmin PLoS One Research Article Thinopyrum elongatum is an important relative of wheat, it is favored by many researchers for the disease resistant genes that exist in its E genome. Some studies have showed that the 7E chromosome of Th. elongatum contains resistance genes related to Fusarium head blight and wheat rust. Therefore, developing 7E chromosome-specific molecular markers linked to resistance genes will provide an important tool for exploring and using the resistant genes of Th. elongatum. In addition, it would greatly contribute in the effort to cultivate disease-resistant wheat varieties. Featured in high throughput, high-accuracy and low-cost, SLAF-seq technology has been widely used in molecular breeding, system evolution, and germplasm resource detection. Based on SLAF-seq, 518 specific fragments on the 7E chromosome of Th. elongatum were successfully amplified. A total of 135 primers were designed according to 135 randomly selected fragments, and 89 specific molecular markers of Th. elongatum were developed, with efficiencies up to 65.9%. These markers were all detected in a variety of materials, and they are all proved to be specific and stable. These markers can be used not only for detecting the 7E chromosome of Th. elongatum but also for providing an important theoretical and practical basis for wheat breeding by marker-assisted selection (MAS). This paper reports the first application of SLAF-seq technology with a high success rate in developing specific molecular markers for Th. elongatum, providing a strong case for the application of this new technology. Public Library of Science 2013-06-10 /pmc/articles/PMC3677899/ /pubmed/23762296 http://dx.doi.org/10.1371/journal.pone.0065122 Text en © 2013 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chen, Shiqiang Huang, Zefeng Dai, Yi Qin, Shuwen Gao, Yingying Zhang, Lulu Gao, Yong Chen, Jianmin The Development of 7E Chromosome-Specific Molecular Markers for Thinopyrum elongatum Based on SLAF-seq Technology |
title | The Development of 7E Chromosome-Specific Molecular Markers for Thinopyrum elongatum Based on SLAF-seq Technology |
title_full | The Development of 7E Chromosome-Specific Molecular Markers for Thinopyrum elongatum Based on SLAF-seq Technology |
title_fullStr | The Development of 7E Chromosome-Specific Molecular Markers for Thinopyrum elongatum Based on SLAF-seq Technology |
title_full_unstemmed | The Development of 7E Chromosome-Specific Molecular Markers for Thinopyrum elongatum Based on SLAF-seq Technology |
title_short | The Development of 7E Chromosome-Specific Molecular Markers for Thinopyrum elongatum Based on SLAF-seq Technology |
title_sort | development of 7e chromosome-specific molecular markers for thinopyrum elongatum based on slaf-seq technology |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3677899/ https://www.ncbi.nlm.nih.gov/pubmed/23762296 http://dx.doi.org/10.1371/journal.pone.0065122 |
work_keys_str_mv | AT chenshiqiang thedevelopmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT huangzefeng thedevelopmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT daiyi thedevelopmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT qinshuwen thedevelopmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT gaoyingying thedevelopmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT zhanglulu thedevelopmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT gaoyong thedevelopmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT chenjianmin thedevelopmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT chenshiqiang developmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT huangzefeng developmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT daiyi developmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT qinshuwen developmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT gaoyingying developmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT zhanglulu developmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT gaoyong developmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology AT chenjianmin developmentof7echromosomespecificmolecularmarkersforthinopyrumelongatumbasedonslafseqtechnology |