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Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China

New Delhi metallo-β-lactamase-1 (NDM-1), an acquired class B carbapenemase, is a significant clinical threat due to its extended hydrolysis of β-lactams including carbapenems. In this study, we identified the first confirmed clinical isolate of Escherichia coli BJ01 harboring bla (NDM-1) in China. T...

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Autores principales: Liu, Zhiyuan, Li, Wei, Wang, Jie, Pan, Jian, Sun, Shipeng, Yu, Yanhua, Zhao, Bing, Ma, Yuzhi, Zhang, Tingju, Qi, Jie, Liu, Guijian, Lu, Fengmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3677923/
https://www.ncbi.nlm.nih.gov/pubmed/23762496
http://dx.doi.org/10.1371/journal.pone.0066666
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author Liu, Zhiyuan
Li, Wei
Wang, Jie
Pan, Jian
Sun, Shipeng
Yu, Yanhua
Zhao, Bing
Ma, Yuzhi
Zhang, Tingju
Qi, Jie
Liu, Guijian
Lu, Fengmin
author_facet Liu, Zhiyuan
Li, Wei
Wang, Jie
Pan, Jian
Sun, Shipeng
Yu, Yanhua
Zhao, Bing
Ma, Yuzhi
Zhang, Tingju
Qi, Jie
Liu, Guijian
Lu, Fengmin
author_sort Liu, Zhiyuan
collection PubMed
description New Delhi metallo-β-lactamase-1 (NDM-1), an acquired class B carbapenemase, is a significant clinical threat due to its extended hydrolysis of β-lactams including carbapenems. In this study, we identified the first confirmed clinical isolate of Escherichia coli BJ01 harboring bla (NDM-1) in China. The isolate is highly resistant to all tested antimicrobials except polymyxin. bla (NDM-1), bla (CTX-M-57), and bla (TEM-1) were identified in the isolate. bla (NDM-1) was transferable to E. coli EC600 and DH5α in both plasmid conjugation experiments and plasmid transformation tests. BJ01 was identified as a new sequence type, ST224, by multilocus sequence typing. Analysis of genetic environment shows complex transposon-like structures surrounding the bla (NDM-1) gene. Genetic analysis revealed that the region flanking bla (NDM-1) was very similar to previously identified NDM-positive Acinetobacter spp. isolated in China. The findings of this study raise attention to the emergence and spread of NDM-1-carrying Enterobacteriaceae in China.
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spelling pubmed-36779232013-06-12 Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China Liu, Zhiyuan Li, Wei Wang, Jie Pan, Jian Sun, Shipeng Yu, Yanhua Zhao, Bing Ma, Yuzhi Zhang, Tingju Qi, Jie Liu, Guijian Lu, Fengmin PLoS One Research Article New Delhi metallo-β-lactamase-1 (NDM-1), an acquired class B carbapenemase, is a significant clinical threat due to its extended hydrolysis of β-lactams including carbapenems. In this study, we identified the first confirmed clinical isolate of Escherichia coli BJ01 harboring bla (NDM-1) in China. The isolate is highly resistant to all tested antimicrobials except polymyxin. bla (NDM-1), bla (CTX-M-57), and bla (TEM-1) were identified in the isolate. bla (NDM-1) was transferable to E. coli EC600 and DH5α in both plasmid conjugation experiments and plasmid transformation tests. BJ01 was identified as a new sequence type, ST224, by multilocus sequence typing. Analysis of genetic environment shows complex transposon-like structures surrounding the bla (NDM-1) gene. Genetic analysis revealed that the region flanking bla (NDM-1) was very similar to previously identified NDM-positive Acinetobacter spp. isolated in China. The findings of this study raise attention to the emergence and spread of NDM-1-carrying Enterobacteriaceae in China. Public Library of Science 2013-06-10 /pmc/articles/PMC3677923/ /pubmed/23762496 http://dx.doi.org/10.1371/journal.pone.0066666 Text en © 2013 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liu, Zhiyuan
Li, Wei
Wang, Jie
Pan, Jian
Sun, Shipeng
Yu, Yanhua
Zhao, Bing
Ma, Yuzhi
Zhang, Tingju
Qi, Jie
Liu, Guijian
Lu, Fengmin
Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China
title Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China
title_full Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China
title_fullStr Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China
title_full_unstemmed Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China
title_short Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China
title_sort identification and characterization of the first escherichia coli strain carrying ndm-1 gene in china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3677923/
https://www.ncbi.nlm.nih.gov/pubmed/23762496
http://dx.doi.org/10.1371/journal.pone.0066666
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