Cargando…

Resveratrol Has Anabolic Effects on Disc Degeneration in a Rabbit Model

This study was done to evaluate whether injections of resveratrol, a natural compound found in the skin of grapes, had anabolic effects on degenerated intervertebral discs in a rabbit model. Two non-continuous lumbar discs were punctured in rabbits to induce disc degeneration. Four weeks and 6 weeks...

Descripción completa

Detalles Bibliográficos
Autor principal: Kwon, Young-Joon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Medical Sciences 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678014/
https://www.ncbi.nlm.nih.gov/pubmed/23772162
http://dx.doi.org/10.3346/jkms.2013.28.6.939
_version_ 1782272793752109056
author Kwon, Young-Joon
author_facet Kwon, Young-Joon
author_sort Kwon, Young-Joon
collection PubMed
description This study was done to evaluate whether injections of resveratrol, a natural compound found in the skin of grapes, had anabolic effects on degenerated intervertebral discs in a rabbit model. Two non-continuous lumbar discs were punctured in rabbits to induce disc degeneration. Four weeks and 6 weeks after puncture, the rabbits were treated by injections with dimethylsulfoxide (DMSO) or resveratrol. At 4, 8, and 16 weeks after initial injection, rabbits were sacrificed and the spine was extracted for magnetic resonance image (MRI), mRNA expression, and histological staining. Resveratrol treatment resulted in stronger signal intensity in T2-weighted images. MRI grade showed significantly lower in the resveratrol group than the DMSO group (P = 0.039). In the resveratrol group, aggrecan gene expression was significantly increased than that in the DMSO group at 16 weeks after injection (P = 0.027). MMP-13 mRNA levels in the resveratrol group were significantly decreased than those in the DMSO group at 8 and 16 weeks (P = 0.006 and P = 0.048, respectively). In hematoxylin and eosin stain, resveratrol-treated discs showed the features of regeneration. Histologic grade revealed improvement in resveratrol-treated discs, compared with DMSO-treated discs (P = 0.024). These anabolic effects on degenerated discs indicate that resveratrol is a promising candidate for treatment of degenerative disc disease.
format Online
Article
Text
id pubmed-3678014
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher The Korean Academy of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-36780142013-06-14 Resveratrol Has Anabolic Effects on Disc Degeneration in a Rabbit Model Kwon, Young-Joon J Korean Med Sci Original Article This study was done to evaluate whether injections of resveratrol, a natural compound found in the skin of grapes, had anabolic effects on degenerated intervertebral discs in a rabbit model. Two non-continuous lumbar discs were punctured in rabbits to induce disc degeneration. Four weeks and 6 weeks after puncture, the rabbits were treated by injections with dimethylsulfoxide (DMSO) or resveratrol. At 4, 8, and 16 weeks after initial injection, rabbits were sacrificed and the spine was extracted for magnetic resonance image (MRI), mRNA expression, and histological staining. Resveratrol treatment resulted in stronger signal intensity in T2-weighted images. MRI grade showed significantly lower in the resveratrol group than the DMSO group (P = 0.039). In the resveratrol group, aggrecan gene expression was significantly increased than that in the DMSO group at 16 weeks after injection (P = 0.027). MMP-13 mRNA levels in the resveratrol group were significantly decreased than those in the DMSO group at 8 and 16 weeks (P = 0.006 and P = 0.048, respectively). In hematoxylin and eosin stain, resveratrol-treated discs showed the features of regeneration. Histologic grade revealed improvement in resveratrol-treated discs, compared with DMSO-treated discs (P = 0.024). These anabolic effects on degenerated discs indicate that resveratrol is a promising candidate for treatment of degenerative disc disease. The Korean Academy of Medical Sciences 2013-06 2013-06-03 /pmc/articles/PMC3678014/ /pubmed/23772162 http://dx.doi.org/10.3346/jkms.2013.28.6.939 Text en © 2013 The Korean Academy of Medical Sciences. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kwon, Young-Joon
Resveratrol Has Anabolic Effects on Disc Degeneration in a Rabbit Model
title Resveratrol Has Anabolic Effects on Disc Degeneration in a Rabbit Model
title_full Resveratrol Has Anabolic Effects on Disc Degeneration in a Rabbit Model
title_fullStr Resveratrol Has Anabolic Effects on Disc Degeneration in a Rabbit Model
title_full_unstemmed Resveratrol Has Anabolic Effects on Disc Degeneration in a Rabbit Model
title_short Resveratrol Has Anabolic Effects on Disc Degeneration in a Rabbit Model
title_sort resveratrol has anabolic effects on disc degeneration in a rabbit model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678014/
https://www.ncbi.nlm.nih.gov/pubmed/23772162
http://dx.doi.org/10.3346/jkms.2013.28.6.939
work_keys_str_mv AT kwonyoungjoon resveratrolhasanaboliceffectsondiscdegenerationinarabbitmodel