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Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins
The eukaryotic genome is highly organized in the nucleus, and this organization affects various nuclear processes. However, the molecular details of higher-order organization of chromatin remain obscure. In the present study, we show that the Saccharomyces cerevisiae silenced loci HML and HMR cluste...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678155/ https://www.ncbi.nlm.nih.gov/pubmed/23733345 http://dx.doi.org/10.1083/jcb.201211105 |
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author | Kirkland, Jacob G. Kamakaka, Rohinton T. |
author_facet | Kirkland, Jacob G. Kamakaka, Rohinton T. |
author_sort | Kirkland, Jacob G. |
collection | PubMed |
description | The eukaryotic genome is highly organized in the nucleus, and this organization affects various nuclear processes. However, the molecular details of higher-order organization of chromatin remain obscure. In the present study, we show that the Saccharomyces cerevisiae silenced loci HML and HMR cluster in three-dimensional space throughout the cell cycle and independently of the telomeres. Long-range HML–HMR interactions require the homologous recombination (HR) repair pathway and phosphorylated H2A (γ-H2A). γ-H2A is constitutively present at silenced loci in unperturbed cells, its localization requires heterochromatin, and it is restricted to the silenced domain by the transfer DNA boundary element. SMC proteins and Scc2 localize to the silenced domain, and Scc2 binding requires the presence of γ-H2A. These findings illustrate a novel pathway for heterochromatin organization and suggest a role for HR repair proteins in genomic organization. |
format | Online Article Text |
id | pubmed-3678155 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-36781552013-12-10 Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins Kirkland, Jacob G. Kamakaka, Rohinton T. J Cell Biol Research Articles The eukaryotic genome is highly organized in the nucleus, and this organization affects various nuclear processes. However, the molecular details of higher-order organization of chromatin remain obscure. In the present study, we show that the Saccharomyces cerevisiae silenced loci HML and HMR cluster in three-dimensional space throughout the cell cycle and independently of the telomeres. Long-range HML–HMR interactions require the homologous recombination (HR) repair pathway and phosphorylated H2A (γ-H2A). γ-H2A is constitutively present at silenced loci in unperturbed cells, its localization requires heterochromatin, and it is restricted to the silenced domain by the transfer DNA boundary element. SMC proteins and Scc2 localize to the silenced domain, and Scc2 binding requires the presence of γ-H2A. These findings illustrate a novel pathway for heterochromatin organization and suggest a role for HR repair proteins in genomic organization. The Rockefeller University Press 2013-06-10 /pmc/articles/PMC3678155/ /pubmed/23733345 http://dx.doi.org/10.1083/jcb.201211105 Text en © 2013 Kirkland and Kamakaka This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Kirkland, Jacob G. Kamakaka, Rohinton T. Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins |
title | Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins |
title_full | Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins |
title_fullStr | Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins |
title_full_unstemmed | Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins |
title_short | Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins |
title_sort | long-range heterochromatin association is mediated by silencing and double-strand dna break repair proteins |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678155/ https://www.ncbi.nlm.nih.gov/pubmed/23733345 http://dx.doi.org/10.1083/jcb.201211105 |
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