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Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins

The eukaryotic genome is highly organized in the nucleus, and this organization affects various nuclear processes. However, the molecular details of higher-order organization of chromatin remain obscure. In the present study, we show that the Saccharomyces cerevisiae silenced loci HML and HMR cluste...

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Detalles Bibliográficos
Autores principales: Kirkland, Jacob G., Kamakaka, Rohinton T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678155/
https://www.ncbi.nlm.nih.gov/pubmed/23733345
http://dx.doi.org/10.1083/jcb.201211105
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author Kirkland, Jacob G.
Kamakaka, Rohinton T.
author_facet Kirkland, Jacob G.
Kamakaka, Rohinton T.
author_sort Kirkland, Jacob G.
collection PubMed
description The eukaryotic genome is highly organized in the nucleus, and this organization affects various nuclear processes. However, the molecular details of higher-order organization of chromatin remain obscure. In the present study, we show that the Saccharomyces cerevisiae silenced loci HML and HMR cluster in three-dimensional space throughout the cell cycle and independently of the telomeres. Long-range HML–HMR interactions require the homologous recombination (HR) repair pathway and phosphorylated H2A (γ-H2A). γ-H2A is constitutively present at silenced loci in unperturbed cells, its localization requires heterochromatin, and it is restricted to the silenced domain by the transfer DNA boundary element. SMC proteins and Scc2 localize to the silenced domain, and Scc2 binding requires the presence of γ-H2A. These findings illustrate a novel pathway for heterochromatin organization and suggest a role for HR repair proteins in genomic organization.
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spelling pubmed-36781552013-12-10 Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins Kirkland, Jacob G. Kamakaka, Rohinton T. J Cell Biol Research Articles The eukaryotic genome is highly organized in the nucleus, and this organization affects various nuclear processes. However, the molecular details of higher-order organization of chromatin remain obscure. In the present study, we show that the Saccharomyces cerevisiae silenced loci HML and HMR cluster in three-dimensional space throughout the cell cycle and independently of the telomeres. Long-range HML–HMR interactions require the homologous recombination (HR) repair pathway and phosphorylated H2A (γ-H2A). γ-H2A is constitutively present at silenced loci in unperturbed cells, its localization requires heterochromatin, and it is restricted to the silenced domain by the transfer DNA boundary element. SMC proteins and Scc2 localize to the silenced domain, and Scc2 binding requires the presence of γ-H2A. These findings illustrate a novel pathway for heterochromatin organization and suggest a role for HR repair proteins in genomic organization. The Rockefeller University Press 2013-06-10 /pmc/articles/PMC3678155/ /pubmed/23733345 http://dx.doi.org/10.1083/jcb.201211105 Text en © 2013 Kirkland and Kamakaka This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Kirkland, Jacob G.
Kamakaka, Rohinton T.
Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins
title Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins
title_full Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins
title_fullStr Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins
title_full_unstemmed Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins
title_short Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins
title_sort long-range heterochromatin association is mediated by silencing and double-strand dna break repair proteins
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678155/
https://www.ncbi.nlm.nih.gov/pubmed/23733345
http://dx.doi.org/10.1083/jcb.201211105
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