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Identification of distinct nisin leader peptide regions that determine interactions with the modification enzymes NisB and NisC()
Nisin is the most prominent and applied bacteriocin that serves as a model for class I lantibiotics. The nisin leader peptide importantly determines interactions between precursor nisin and its modification enzymes NisB and NisC that mature nisin posttranslationally. NisB dehydrates serines and thre...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678300/ https://www.ncbi.nlm.nih.gov/pubmed/23772400 http://dx.doi.org/10.1016/j.fob.2013.05.001 |
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author | Khusainov, Rustem Moll, Gert N. Kuipers, Oscar P. |
author_facet | Khusainov, Rustem Moll, Gert N. Kuipers, Oscar P. |
author_sort | Khusainov, Rustem |
collection | PubMed |
description | Nisin is the most prominent and applied bacteriocin that serves as a model for class I lantibiotics. The nisin leader peptide importantly determines interactions between precursor nisin and its modification enzymes NisB and NisC that mature nisin posttranslationally. NisB dehydrates serines and threonines, while NisC catalyzes the subsequent coupling of the formed dehydroamino acids to form lanthionines. Currently, little is known about how the nisin leader interacts with NisB and even less is known about its interactions with NisC. To investigate the nisin leader peptide requirements for functional interaction with the modification enzymes NisB and NisC, we systematically replaced six regions, of 2–4 amino acids each, with all-alanine regions. By performing NisB and NisC co-purification studies with these mutant leader peptides, we demonstrate that the nisin leader regions STKD(-22-19), FNLD(-18-15) and PR(-2-1) importantly contribute to the interactions of precursor nisin with both NisB and NisC, whereas the nisin leader region LVSV(-14-11) additionally contributes to the interaction of precursor nisin with NisC. |
format | Online Article Text |
id | pubmed-3678300 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-36783002013-06-14 Identification of distinct nisin leader peptide regions that determine interactions with the modification enzymes NisB and NisC() Khusainov, Rustem Moll, Gert N. Kuipers, Oscar P. FEBS Open Bio Article Nisin is the most prominent and applied bacteriocin that serves as a model for class I lantibiotics. The nisin leader peptide importantly determines interactions between precursor nisin and its modification enzymes NisB and NisC that mature nisin posttranslationally. NisB dehydrates serines and threonines, while NisC catalyzes the subsequent coupling of the formed dehydroamino acids to form lanthionines. Currently, little is known about how the nisin leader interacts with NisB and even less is known about its interactions with NisC. To investigate the nisin leader peptide requirements for functional interaction with the modification enzymes NisB and NisC, we systematically replaced six regions, of 2–4 amino acids each, with all-alanine regions. By performing NisB and NisC co-purification studies with these mutant leader peptides, we demonstrate that the nisin leader regions STKD(-22-19), FNLD(-18-15) and PR(-2-1) importantly contribute to the interactions of precursor nisin with both NisB and NisC, whereas the nisin leader region LVSV(-14-11) additionally contributes to the interaction of precursor nisin with NisC. Elsevier 2013-05-30 /pmc/articles/PMC3678300/ /pubmed/23772400 http://dx.doi.org/10.1016/j.fob.2013.05.001 Text en © 2013 The Authors http://creativecommons.org/licenses/BY-NC-ND/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Article Khusainov, Rustem Moll, Gert N. Kuipers, Oscar P. Identification of distinct nisin leader peptide regions that determine interactions with the modification enzymes NisB and NisC() |
title | Identification of distinct nisin leader peptide regions that determine interactions with the modification enzymes NisB and NisC() |
title_full | Identification of distinct nisin leader peptide regions that determine interactions with the modification enzymes NisB and NisC() |
title_fullStr | Identification of distinct nisin leader peptide regions that determine interactions with the modification enzymes NisB and NisC() |
title_full_unstemmed | Identification of distinct nisin leader peptide regions that determine interactions with the modification enzymes NisB and NisC() |
title_short | Identification of distinct nisin leader peptide regions that determine interactions with the modification enzymes NisB and NisC() |
title_sort | identification of distinct nisin leader peptide regions that determine interactions with the modification enzymes nisb and nisc() |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678300/ https://www.ncbi.nlm.nih.gov/pubmed/23772400 http://dx.doi.org/10.1016/j.fob.2013.05.001 |
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