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Ovine uterine space restriction alters placental transferrin receptor and fetal iron status during late pregnancy
BACKGROUND: Fetal growth restriction is reported to be associated with impaired placental iron transport. Transferrin receptor (TfR) is a major placental iron transporter in humans, but is unstudied in sheep. TfR is regulated by both iron and nitric oxide (NO), the molecule produced by endothelial N...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678369/ https://www.ncbi.nlm.nih.gov/pubmed/23202722 http://dx.doi.org/10.1038/pr.2012.180 |
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author | Sun, Mary Y. Habeck, Jason M. Meyer, Katie M. Koch, Jill M. Ramadoss, Jayanth Blohowiak, Sharon E. Magness, Ronald R. Kling, Pamela J. |
author_facet | Sun, Mary Y. Habeck, Jason M. Meyer, Katie M. Koch, Jill M. Ramadoss, Jayanth Blohowiak, Sharon E. Magness, Ronald R. Kling, Pamela J. |
author_sort | Sun, Mary Y. |
collection | PubMed |
description | BACKGROUND: Fetal growth restriction is reported to be associated with impaired placental iron transport. Transferrin receptor (TfR) is a major placental iron transporter in humans, but is unstudied in sheep. TfR is regulated by both iron and nitric oxide (NO), the molecule produced by endothelial NOS (eNOS). We hypothesized that limited placental development downregulates both placental TfR and eNOS expression, thereby lowering fetal tissue iron. METHODS: An ovine surgical uterine space restriction (USR) model, combined with multifetal gestation, tested the extremes of uterine and placental adaptation. Blood, tissues, and placentomes from non-space restricted (NSR) singletons were compared to USR fetuses at 120 or 130 days of gestation (GD). RESULTS: When expressed proportionate to fetal weight, liver iron content did not differ while renal iron was higher in USR vs. NSR fetuses. Renal TfR protein expression did not differ, but placental TfR expression was lower in USR fetuses at GD130. Placental levels of TfR correlated to eNOS. TfR was localized throughout the placentome, including the hemophagous zone, implicating a role for TfR in ovine placental iron transport. CONCLUSION: In conclusion, fetal iron was regulated in an organ-specific fashion. In USR fetuses, NO-mediated placental adaptations may prevent the normal upregulation of placental TfR at GD130. |
format | Online Article Text |
id | pubmed-3678369 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
record_format | MEDLINE/PubMed |
spelling | pubmed-36783692013-09-01 Ovine uterine space restriction alters placental transferrin receptor and fetal iron status during late pregnancy Sun, Mary Y. Habeck, Jason M. Meyer, Katie M. Koch, Jill M. Ramadoss, Jayanth Blohowiak, Sharon E. Magness, Ronald R. Kling, Pamela J. Pediatr Res Article BACKGROUND: Fetal growth restriction is reported to be associated with impaired placental iron transport. Transferrin receptor (TfR) is a major placental iron transporter in humans, but is unstudied in sheep. TfR is regulated by both iron and nitric oxide (NO), the molecule produced by endothelial NOS (eNOS). We hypothesized that limited placental development downregulates both placental TfR and eNOS expression, thereby lowering fetal tissue iron. METHODS: An ovine surgical uterine space restriction (USR) model, combined with multifetal gestation, tested the extremes of uterine and placental adaptation. Blood, tissues, and placentomes from non-space restricted (NSR) singletons were compared to USR fetuses at 120 or 130 days of gestation (GD). RESULTS: When expressed proportionate to fetal weight, liver iron content did not differ while renal iron was higher in USR vs. NSR fetuses. Renal TfR protein expression did not differ, but placental TfR expression was lower in USR fetuses at GD130. Placental levels of TfR correlated to eNOS. TfR was localized throughout the placentome, including the hemophagous zone, implicating a role for TfR in ovine placental iron transport. CONCLUSION: In conclusion, fetal iron was regulated in an organ-specific fashion. In USR fetuses, NO-mediated placental adaptations may prevent the normal upregulation of placental TfR at GD130. 2012-11-30 2013-03 /pmc/articles/PMC3678369/ /pubmed/23202722 http://dx.doi.org/10.1038/pr.2012.180 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Sun, Mary Y. Habeck, Jason M. Meyer, Katie M. Koch, Jill M. Ramadoss, Jayanth Blohowiak, Sharon E. Magness, Ronald R. Kling, Pamela J. Ovine uterine space restriction alters placental transferrin receptor and fetal iron status during late pregnancy |
title | Ovine uterine space restriction alters placental transferrin receptor and fetal iron status during late pregnancy |
title_full | Ovine uterine space restriction alters placental transferrin receptor and fetal iron status during late pregnancy |
title_fullStr | Ovine uterine space restriction alters placental transferrin receptor and fetal iron status during late pregnancy |
title_full_unstemmed | Ovine uterine space restriction alters placental transferrin receptor and fetal iron status during late pregnancy |
title_short | Ovine uterine space restriction alters placental transferrin receptor and fetal iron status during late pregnancy |
title_sort | ovine uterine space restriction alters placental transferrin receptor and fetal iron status during late pregnancy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678369/ https://www.ncbi.nlm.nih.gov/pubmed/23202722 http://dx.doi.org/10.1038/pr.2012.180 |
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