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The Extract of Litsea japonica Reduced the Development of Diabetic Nephropathy via the Inhibition of Advanced Glycation End Products Accumulation in db/db Mice

Increasing evidence indicates that advanced glycation end products (AGEs) contribute to the pathogenesis of diabetic nephropathy. The aim of this study was to investigate the protective effect of L. japonica extract (LJE) against renal damage in the db/db mouse. LJE (100 or 250 mg/kg per day) was gi...

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Autores principales: Sohn, Eunjin, Kim, Junghyun, Kim, Chan-Sik, Lee, Yun Mi, Jo, Kyuhyung, Shin, So Dam, Kim, Joo Hwan, Kim, Jin Sook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678449/
https://www.ncbi.nlm.nih.gov/pubmed/23781268
http://dx.doi.org/10.1155/2013/769416
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author Sohn, Eunjin
Kim, Junghyun
Kim, Chan-Sik
Lee, Yun Mi
Jo, Kyuhyung
Shin, So Dam
Kim, Joo Hwan
Kim, Jin Sook
author_facet Sohn, Eunjin
Kim, Junghyun
Kim, Chan-Sik
Lee, Yun Mi
Jo, Kyuhyung
Shin, So Dam
Kim, Joo Hwan
Kim, Jin Sook
author_sort Sohn, Eunjin
collection PubMed
description Increasing evidence indicates that advanced glycation end products (AGEs) contribute to the pathogenesis of diabetic nephropathy. The aim of this study was to investigate the protective effect of L. japonica extract (LJE) against renal damage in the db/db mouse. LJE (100 or 250 mg/kg per day) was given to diabetic mice for 12 weeks. Body weight, blood glucose levels, glycated hemoglobin (HbA1c) levels, and proteinuria were examined. In in vitro assay of the inhibition of AGE formation, immunohistochemical analysis of podocyte loss and AGE accumulations were performed. In 20-week-old db/db mice, severe hyperglycemia developed, and proteinuria was significantly increased. Diabetes induced markedly morphological alterations to the renal glomerular cells. AGE accumulations and podocyte loss were detected in renal glomeruli. LJE treatment significantly reduced proteinuria and AGE accumulations in diabetic mice. Moreover, the loss of nephrin, an important slit diaphragm component in the kidneys, was restored by LJE treatment. Our studies suggest that LJE might be beneficial for the treatment of diabetic nephropathy. The ability of LJE to attenuate proteinuria and podocyte dysfunction may be mediated by the inhibition of AGE accumulation in the context of diabetic nephropathy in db/db mice.
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spelling pubmed-36784492013-06-18 The Extract of Litsea japonica Reduced the Development of Diabetic Nephropathy via the Inhibition of Advanced Glycation End Products Accumulation in db/db Mice Sohn, Eunjin Kim, Junghyun Kim, Chan-Sik Lee, Yun Mi Jo, Kyuhyung Shin, So Dam Kim, Joo Hwan Kim, Jin Sook Evid Based Complement Alternat Med Research Article Increasing evidence indicates that advanced glycation end products (AGEs) contribute to the pathogenesis of diabetic nephropathy. The aim of this study was to investigate the protective effect of L. japonica extract (LJE) against renal damage in the db/db mouse. LJE (100 or 250 mg/kg per day) was given to diabetic mice for 12 weeks. Body weight, blood glucose levels, glycated hemoglobin (HbA1c) levels, and proteinuria were examined. In in vitro assay of the inhibition of AGE formation, immunohistochemical analysis of podocyte loss and AGE accumulations were performed. In 20-week-old db/db mice, severe hyperglycemia developed, and proteinuria was significantly increased. Diabetes induced markedly morphological alterations to the renal glomerular cells. AGE accumulations and podocyte loss were detected in renal glomeruli. LJE treatment significantly reduced proteinuria and AGE accumulations in diabetic mice. Moreover, the loss of nephrin, an important slit diaphragm component in the kidneys, was restored by LJE treatment. Our studies suggest that LJE might be beneficial for the treatment of diabetic nephropathy. The ability of LJE to attenuate proteinuria and podocyte dysfunction may be mediated by the inhibition of AGE accumulation in the context of diabetic nephropathy in db/db mice. Hindawi Publishing Corporation 2013 2013-05-27 /pmc/articles/PMC3678449/ /pubmed/23781268 http://dx.doi.org/10.1155/2013/769416 Text en Copyright © 2013 Eunjin Sohn et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sohn, Eunjin
Kim, Junghyun
Kim, Chan-Sik
Lee, Yun Mi
Jo, Kyuhyung
Shin, So Dam
Kim, Joo Hwan
Kim, Jin Sook
The Extract of Litsea japonica Reduced the Development of Diabetic Nephropathy via the Inhibition of Advanced Glycation End Products Accumulation in db/db Mice
title The Extract of Litsea japonica Reduced the Development of Diabetic Nephropathy via the Inhibition of Advanced Glycation End Products Accumulation in db/db Mice
title_full The Extract of Litsea japonica Reduced the Development of Diabetic Nephropathy via the Inhibition of Advanced Glycation End Products Accumulation in db/db Mice
title_fullStr The Extract of Litsea japonica Reduced the Development of Diabetic Nephropathy via the Inhibition of Advanced Glycation End Products Accumulation in db/db Mice
title_full_unstemmed The Extract of Litsea japonica Reduced the Development of Diabetic Nephropathy via the Inhibition of Advanced Glycation End Products Accumulation in db/db Mice
title_short The Extract of Litsea japonica Reduced the Development of Diabetic Nephropathy via the Inhibition of Advanced Glycation End Products Accumulation in db/db Mice
title_sort extract of litsea japonica reduced the development of diabetic nephropathy via the inhibition of advanced glycation end products accumulation in db/db mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678449/
https://www.ncbi.nlm.nih.gov/pubmed/23781268
http://dx.doi.org/10.1155/2013/769416
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