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Eukaryotic Initiation Factor 4H Is under Transcriptional Control of p65/NF-κB

Protein synthesis is mainly regulated at the initiation step, allowing the fast, reversible and spatial control of gene expression. Initiation of protein synthesis requires at least 13 translation initiation factors to assemble the 80S ribosomal initiation complex. Loss of translation control may re...

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Autores principales: Fiume, Giuseppe, Rossi, Annalisa, de Laurentiis, Annamaria, Falcone, Cristina, Pisano, Antonio, Vecchio, Eleonora, Pontoriero, Marilena, Scala, Iris, Scialdone, Annarita, Masci, Francesca Fasanella, Mimmi, Selena, Palmieri, Camillo, Scala, Giuseppe, Quinto, Ileana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679033/
https://www.ncbi.nlm.nih.gov/pubmed/23776612
http://dx.doi.org/10.1371/journal.pone.0066087
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author Fiume, Giuseppe
Rossi, Annalisa
de Laurentiis, Annamaria
Falcone, Cristina
Pisano, Antonio
Vecchio, Eleonora
Pontoriero, Marilena
Scala, Iris
Scialdone, Annarita
Masci, Francesca Fasanella
Mimmi, Selena
Palmieri, Camillo
Scala, Giuseppe
Quinto, Ileana
author_facet Fiume, Giuseppe
Rossi, Annalisa
de Laurentiis, Annamaria
Falcone, Cristina
Pisano, Antonio
Vecchio, Eleonora
Pontoriero, Marilena
Scala, Iris
Scialdone, Annarita
Masci, Francesca Fasanella
Mimmi, Selena
Palmieri, Camillo
Scala, Giuseppe
Quinto, Ileana
author_sort Fiume, Giuseppe
collection PubMed
description Protein synthesis is mainly regulated at the initiation step, allowing the fast, reversible and spatial control of gene expression. Initiation of protein synthesis requires at least 13 translation initiation factors to assemble the 80S ribosomal initiation complex. Loss of translation control may result in cell malignant transformation. Here, we asked whether translational initiation factors could be regulated by NF-κB transcription factor, a major regulator of genes involved in cell proliferation, survival, and inflammatory response. We show that the p65 subunit of NF-κB activates the transcription of eIF4H gene, which is the regulatory subunit of eIF4A, the most relevant RNA helicase in translation initiation. The p65-dependent transcriptional activation of eIF4H increased the eIF4H protein content augmenting the rate of global protein synthesis. In this context, our results provide novel insights into protein synthesis regulation in response to NF-κB activation signalling, suggesting a transcription-translation coupled mechanism of control.
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spelling pubmed-36790332013-06-17 Eukaryotic Initiation Factor 4H Is under Transcriptional Control of p65/NF-κB Fiume, Giuseppe Rossi, Annalisa de Laurentiis, Annamaria Falcone, Cristina Pisano, Antonio Vecchio, Eleonora Pontoriero, Marilena Scala, Iris Scialdone, Annarita Masci, Francesca Fasanella Mimmi, Selena Palmieri, Camillo Scala, Giuseppe Quinto, Ileana PLoS One Research Article Protein synthesis is mainly regulated at the initiation step, allowing the fast, reversible and spatial control of gene expression. Initiation of protein synthesis requires at least 13 translation initiation factors to assemble the 80S ribosomal initiation complex. Loss of translation control may result in cell malignant transformation. Here, we asked whether translational initiation factors could be regulated by NF-κB transcription factor, a major regulator of genes involved in cell proliferation, survival, and inflammatory response. We show that the p65 subunit of NF-κB activates the transcription of eIF4H gene, which is the regulatory subunit of eIF4A, the most relevant RNA helicase in translation initiation. The p65-dependent transcriptional activation of eIF4H increased the eIF4H protein content augmenting the rate of global protein synthesis. In this context, our results provide novel insights into protein synthesis regulation in response to NF-κB activation signalling, suggesting a transcription-translation coupled mechanism of control. Public Library of Science 2013-06-11 /pmc/articles/PMC3679033/ /pubmed/23776612 http://dx.doi.org/10.1371/journal.pone.0066087 Text en © 2013 Fiume et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fiume, Giuseppe
Rossi, Annalisa
de Laurentiis, Annamaria
Falcone, Cristina
Pisano, Antonio
Vecchio, Eleonora
Pontoriero, Marilena
Scala, Iris
Scialdone, Annarita
Masci, Francesca Fasanella
Mimmi, Selena
Palmieri, Camillo
Scala, Giuseppe
Quinto, Ileana
Eukaryotic Initiation Factor 4H Is under Transcriptional Control of p65/NF-κB
title Eukaryotic Initiation Factor 4H Is under Transcriptional Control of p65/NF-κB
title_full Eukaryotic Initiation Factor 4H Is under Transcriptional Control of p65/NF-κB
title_fullStr Eukaryotic Initiation Factor 4H Is under Transcriptional Control of p65/NF-κB
title_full_unstemmed Eukaryotic Initiation Factor 4H Is under Transcriptional Control of p65/NF-κB
title_short Eukaryotic Initiation Factor 4H Is under Transcriptional Control of p65/NF-κB
title_sort eukaryotic initiation factor 4h is under transcriptional control of p65/nf-κb
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679033/
https://www.ncbi.nlm.nih.gov/pubmed/23776612
http://dx.doi.org/10.1371/journal.pone.0066087
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