Multilocus Sequence Typing (MLST) for Characterization of Enterobacter cloacae

Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the...

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Detalles Bibliográficos
Autores principales: Miyoshi-Akiyama, Tohru, Hayakawa, Kayoko, Ohmagari, Norio, Shimojima, Masahiro, Kirikae, Teruo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679064/
https://www.ncbi.nlm.nih.gov/pubmed/23776664
http://dx.doi.org/10.1371/journal.pone.0066358
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author Miyoshi-Akiyama, Tohru
Hayakawa, Kayoko
Ohmagari, Norio
Shimojima, Masahiro
Kirikae, Teruo
author_facet Miyoshi-Akiyama, Tohru
Hayakawa, Kayoko
Ohmagari, Norio
Shimojima, Masahiro
Kirikae, Teruo
author_sort Miyoshi-Akiyama, Tohru
collection PubMed
description Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the performance in 101 clinical isolates. The MLST scheme yielded 83 sequence types (ST) including 78 novel STs found in the clinical isolates. These findings supported the robustness of the MLST scheme developed in this study.
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spelling pubmed-36790642013-06-17 Multilocus Sequence Typing (MLST) for Characterization of Enterobacter cloacae Miyoshi-Akiyama, Tohru Hayakawa, Kayoko Ohmagari, Norio Shimojima, Masahiro Kirikae, Teruo PLoS One Research Article Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the performance in 101 clinical isolates. The MLST scheme yielded 83 sequence types (ST) including 78 novel STs found in the clinical isolates. These findings supported the robustness of the MLST scheme developed in this study. Public Library of Science 2013-06-11 /pmc/articles/PMC3679064/ /pubmed/23776664 http://dx.doi.org/10.1371/journal.pone.0066358 Text en © 2013 Miyoshi-Akiyama et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Miyoshi-Akiyama, Tohru
Hayakawa, Kayoko
Ohmagari, Norio
Shimojima, Masahiro
Kirikae, Teruo
Multilocus Sequence Typing (MLST) for Characterization of Enterobacter cloacae
title Multilocus Sequence Typing (MLST) for Characterization of Enterobacter cloacae
title_full Multilocus Sequence Typing (MLST) for Characterization of Enterobacter cloacae
title_fullStr Multilocus Sequence Typing (MLST) for Characterization of Enterobacter cloacae
title_full_unstemmed Multilocus Sequence Typing (MLST) for Characterization of Enterobacter cloacae
title_short Multilocus Sequence Typing (MLST) for Characterization of Enterobacter cloacae
title_sort multilocus sequence typing (mlst) for characterization of enterobacter cloacae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679064/
https://www.ncbi.nlm.nih.gov/pubmed/23776664
http://dx.doi.org/10.1371/journal.pone.0066358
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