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Agonist Activated PKCβ(II) Translocation and Modulation of Cardiac Myocyte Contractile Function
Elevated protein kinase C β(II) (PKCβ(II)) expression develops during heart failure and yet the role of this isoform in modulating contractile function remains controversial. The present study examines the impact of agonist-induced PKCβ(II) activation on contractile function in adult cardiac myocyte...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679501/ https://www.ncbi.nlm.nih.gov/pubmed/23756828 http://dx.doi.org/10.1038/srep01971 |
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author | Hwang, Hyosook Robinson, Dustin Rogers, Julie B. Stevenson, Tamara K. Lang, Sarah E. Sadayappan, Sakthivel Day, Sharlene M. Sivaramakrishnan, Sivaraj Westfall, Margaret V. |
author_facet | Hwang, Hyosook Robinson, Dustin Rogers, Julie B. Stevenson, Tamara K. Lang, Sarah E. Sadayappan, Sakthivel Day, Sharlene M. Sivaramakrishnan, Sivaraj Westfall, Margaret V. |
author_sort | Hwang, Hyosook |
collection | PubMed |
description | Elevated protein kinase C β(II) (PKCβ(II)) expression develops during heart failure and yet the role of this isoform in modulating contractile function remains controversial. The present study examines the impact of agonist-induced PKCβ(II) activation on contractile function in adult cardiac myocytes. Diminished contractile function develops in response to low dose phenylephrine (PHE, 100 nM) in controls, while function is preserved in response to PHE in PKCβ(II)-expressing myocytes. PHE also caused PKCβ(II) translocation and a punctate distribution pattern in myocytes expressing this isoform. The preserved contractile function and translocation responses to PHE are blocked by the inhibitor, LY379196 (30 nM) in PKCβ(II)-expressing myocytes. Further analysis showed downstream protein kinase D (PKD) phosphorylation and phosphatase activation are associated with the LY379196-sensitive contractile response. PHE also triggered a complex pattern of end-target phosphorylation in PKCβ(II)-expressing myocytes. These patterns are consistent with bifurcated activation of downstream signaling activity by PKCβ(II). |
format | Online Article Text |
id | pubmed-3679501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-36795012013-06-12 Agonist Activated PKCβ(II) Translocation and Modulation of Cardiac Myocyte Contractile Function Hwang, Hyosook Robinson, Dustin Rogers, Julie B. Stevenson, Tamara K. Lang, Sarah E. Sadayappan, Sakthivel Day, Sharlene M. Sivaramakrishnan, Sivaraj Westfall, Margaret V. Sci Rep Article Elevated protein kinase C β(II) (PKCβ(II)) expression develops during heart failure and yet the role of this isoform in modulating contractile function remains controversial. The present study examines the impact of agonist-induced PKCβ(II) activation on contractile function in adult cardiac myocytes. Diminished contractile function develops in response to low dose phenylephrine (PHE, 100 nM) in controls, while function is preserved in response to PHE in PKCβ(II)-expressing myocytes. PHE also caused PKCβ(II) translocation and a punctate distribution pattern in myocytes expressing this isoform. The preserved contractile function and translocation responses to PHE are blocked by the inhibitor, LY379196 (30 nM) in PKCβ(II)-expressing myocytes. Further analysis showed downstream protein kinase D (PKD) phosphorylation and phosphatase activation are associated with the LY379196-sensitive contractile response. PHE also triggered a complex pattern of end-target phosphorylation in PKCβ(II)-expressing myocytes. These patterns are consistent with bifurcated activation of downstream signaling activity by PKCβ(II). Nature Publishing Group 2013-06-12 /pmc/articles/PMC3679501/ /pubmed/23756828 http://dx.doi.org/10.1038/srep01971 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Hwang, Hyosook Robinson, Dustin Rogers, Julie B. Stevenson, Tamara K. Lang, Sarah E. Sadayappan, Sakthivel Day, Sharlene M. Sivaramakrishnan, Sivaraj Westfall, Margaret V. Agonist Activated PKCβ(II) Translocation and Modulation of Cardiac Myocyte Contractile Function |
title | Agonist Activated PKCβ(II) Translocation and Modulation of Cardiac Myocyte Contractile Function |
title_full | Agonist Activated PKCβ(II) Translocation and Modulation of Cardiac Myocyte Contractile Function |
title_fullStr | Agonist Activated PKCβ(II) Translocation and Modulation of Cardiac Myocyte Contractile Function |
title_full_unstemmed | Agonist Activated PKCβ(II) Translocation and Modulation of Cardiac Myocyte Contractile Function |
title_short | Agonist Activated PKCβ(II) Translocation and Modulation of Cardiac Myocyte Contractile Function |
title_sort | agonist activated pkcβ(ii) translocation and modulation of cardiac myocyte contractile function |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679501/ https://www.ncbi.nlm.nih.gov/pubmed/23756828 http://dx.doi.org/10.1038/srep01971 |
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