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Propolis Modifies Collagen Types I and III Accumulation in the Matrix of Burnt Tissue

Wound healing represents an interactive process which requires highly organized activity of various cells, synthesizing cytokines, growth factors, and collagen. Collagen types I and III, serving as structural and regulatory molecules, play pivotal roles during wound healing. The aim of this study wa...

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Autores principales: Olczyk, Pawel, Wisowski, Grzegorz, Komosinska-Vassev, Katarzyna, Stojko, Jerzy, Klimek, Katarzyna, Olczyk, Monika, Kozma, Ewa M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679764/
https://www.ncbi.nlm.nih.gov/pubmed/23781260
http://dx.doi.org/10.1155/2013/423809
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author Olczyk, Pawel
Wisowski, Grzegorz
Komosinska-Vassev, Katarzyna
Stojko, Jerzy
Klimek, Katarzyna
Olczyk, Monika
Kozma, Ewa M.
author_facet Olczyk, Pawel
Wisowski, Grzegorz
Komosinska-Vassev, Katarzyna
Stojko, Jerzy
Klimek, Katarzyna
Olczyk, Monika
Kozma, Ewa M.
author_sort Olczyk, Pawel
collection PubMed
description Wound healing represents an interactive process which requires highly organized activity of various cells, synthesizing cytokines, growth factors, and collagen. Collagen types I and III, serving as structural and regulatory molecules, play pivotal roles during wound healing. The aim of this study was to compare the propolis and silver sulfadiazine therapeutic efficacy throughout the quantitative and qualitative assessment of collagen types I and III accumulation in the matrix of burnt tissues. Burn wounds were inflicted on pigs, chosen for the evaluation of wound repair because of many similarities between pig and human skin. Isolated collagen types I and III were estimated by the surface plasmon resonance method with a subsequent collagenous quantification using electrophoretic and densitometric analyses. Propolis burn treatment led to enhanced collagens and its components expression, especially during the initial stage of the study. Less expressed changes were observed after silver sulfadiazine (AgSD) application. AgSD and, with a smaller intensity, propolis stimulated accumulation of collagenous degradation products. The assessed propolis therapeutic efficacy, throughout quantitatively and qualitatively analyses of collagen types I and III expression and degradation in wounds matrix, may indicate that apitherapeutic agent can generate favorable biochemical environment supporting reepithelization.
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spelling pubmed-36797642013-06-18 Propolis Modifies Collagen Types I and III Accumulation in the Matrix of Burnt Tissue Olczyk, Pawel Wisowski, Grzegorz Komosinska-Vassev, Katarzyna Stojko, Jerzy Klimek, Katarzyna Olczyk, Monika Kozma, Ewa M. Evid Based Complement Alternat Med Research Article Wound healing represents an interactive process which requires highly organized activity of various cells, synthesizing cytokines, growth factors, and collagen. Collagen types I and III, serving as structural and regulatory molecules, play pivotal roles during wound healing. The aim of this study was to compare the propolis and silver sulfadiazine therapeutic efficacy throughout the quantitative and qualitative assessment of collagen types I and III accumulation in the matrix of burnt tissues. Burn wounds were inflicted on pigs, chosen for the evaluation of wound repair because of many similarities between pig and human skin. Isolated collagen types I and III were estimated by the surface plasmon resonance method with a subsequent collagenous quantification using electrophoretic and densitometric analyses. Propolis burn treatment led to enhanced collagens and its components expression, especially during the initial stage of the study. Less expressed changes were observed after silver sulfadiazine (AgSD) application. AgSD and, with a smaller intensity, propolis stimulated accumulation of collagenous degradation products. The assessed propolis therapeutic efficacy, throughout quantitatively and qualitatively analyses of collagen types I and III expression and degradation in wounds matrix, may indicate that apitherapeutic agent can generate favorable biochemical environment supporting reepithelization. Hindawi Publishing Corporation 2013 2013-05-28 /pmc/articles/PMC3679764/ /pubmed/23781260 http://dx.doi.org/10.1155/2013/423809 Text en Copyright © 2013 Pawel Olczyk et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Olczyk, Pawel
Wisowski, Grzegorz
Komosinska-Vassev, Katarzyna
Stojko, Jerzy
Klimek, Katarzyna
Olczyk, Monika
Kozma, Ewa M.
Propolis Modifies Collagen Types I and III Accumulation in the Matrix of Burnt Tissue
title Propolis Modifies Collagen Types I and III Accumulation in the Matrix of Burnt Tissue
title_full Propolis Modifies Collagen Types I and III Accumulation in the Matrix of Burnt Tissue
title_fullStr Propolis Modifies Collagen Types I and III Accumulation in the Matrix of Burnt Tissue
title_full_unstemmed Propolis Modifies Collagen Types I and III Accumulation in the Matrix of Burnt Tissue
title_short Propolis Modifies Collagen Types I and III Accumulation in the Matrix of Burnt Tissue
title_sort propolis modifies collagen types i and iii accumulation in the matrix of burnt tissue
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679764/
https://www.ncbi.nlm.nih.gov/pubmed/23781260
http://dx.doi.org/10.1155/2013/423809
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