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Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients
BACKGROUND: The genus Pseudomonas includes a heterogeneous set of microorganisms that can be isolated from many different niches and nearly 100 different strains have been described. The best characterized bacterium is Pseudomonas aeruginosa which is the primary agent of opportunistic infection in h...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679952/ https://www.ncbi.nlm.nih.gov/pubmed/23718251 http://dx.doi.org/10.1186/1471-2180-13-123 |
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author | Leneveu-Jenvrin, Charlène Madi, Amar Bouffartigues, Emeline Biaggini, Kelly Feuilloley, Marc Chevalier, Sylvie Connil, Nathalie |
author_facet | Leneveu-Jenvrin, Charlène Madi, Amar Bouffartigues, Emeline Biaggini, Kelly Feuilloley, Marc Chevalier, Sylvie Connil, Nathalie |
author_sort | Leneveu-Jenvrin, Charlène |
collection | PubMed |
description | BACKGROUND: The genus Pseudomonas includes a heterogeneous set of microorganisms that can be isolated from many different niches and nearly 100 different strains have been described. The best characterized bacterium is Pseudomonas aeruginosa which is the primary agent of opportunistic infection in humans, causing both acute and chronic infections. Other species like fluorescens, putida or mosselii have been sporadically isolated from hospitalized patients but their association with the pathology often remains unclear. RESULTS: This study focuses on the cytotoxicity and inflammatory potential of two strains of Pseudomonas mosselii (ATCC BAA-99 and MFY161) that were recently isolated from clinical samples of hospitalized patients. The behavior of these bacteria was compared to that of the well-known opportunistic pathogen P. aeruginosa PAO1. We found that P. mosselii ATCC BAA-99 and MFY161 are cytotoxic towards Caco-2/TC7 cells, have low invasive capacity, induce secretion of human β-defensin 2 (HBD-2), alter the epithelial permeability of differentiated cells and damage the F-actin cytoskeleton. CONCLUSIONS: These data bring new insights into P. mosselii virulence, since this bacterium has often been neglected due to its rare occurrence in hospital. |
format | Online Article Text |
id | pubmed-3679952 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-36799522013-06-13 Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients Leneveu-Jenvrin, Charlène Madi, Amar Bouffartigues, Emeline Biaggini, Kelly Feuilloley, Marc Chevalier, Sylvie Connil, Nathalie BMC Microbiol Research Article BACKGROUND: The genus Pseudomonas includes a heterogeneous set of microorganisms that can be isolated from many different niches and nearly 100 different strains have been described. The best characterized bacterium is Pseudomonas aeruginosa which is the primary agent of opportunistic infection in humans, causing both acute and chronic infections. Other species like fluorescens, putida or mosselii have been sporadically isolated from hospitalized patients but their association with the pathology often remains unclear. RESULTS: This study focuses on the cytotoxicity and inflammatory potential of two strains of Pseudomonas mosselii (ATCC BAA-99 and MFY161) that were recently isolated from clinical samples of hospitalized patients. The behavior of these bacteria was compared to that of the well-known opportunistic pathogen P. aeruginosa PAO1. We found that P. mosselii ATCC BAA-99 and MFY161 are cytotoxic towards Caco-2/TC7 cells, have low invasive capacity, induce secretion of human β-defensin 2 (HBD-2), alter the epithelial permeability of differentiated cells and damage the F-actin cytoskeleton. CONCLUSIONS: These data bring new insights into P. mosselii virulence, since this bacterium has often been neglected due to its rare occurrence in hospital. BioMed Central 2013-05-29 /pmc/articles/PMC3679952/ /pubmed/23718251 http://dx.doi.org/10.1186/1471-2180-13-123 Text en Copyright © 2013 Leneveu-Jenvrin et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Leneveu-Jenvrin, Charlène Madi, Amar Bouffartigues, Emeline Biaggini, Kelly Feuilloley, Marc Chevalier, Sylvie Connil, Nathalie Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients |
title | Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients |
title_full | Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients |
title_fullStr | Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients |
title_full_unstemmed | Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients |
title_short | Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients |
title_sort | cytotoxicity and inflammatory potential of two pseudomonas mosselii strains isolated from clinical samples of hospitalized patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679952/ https://www.ncbi.nlm.nih.gov/pubmed/23718251 http://dx.doi.org/10.1186/1471-2180-13-123 |
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