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Strategy for successful expression of the Pseudomonas putida nitrile hydratase activator P14K in Escherichia coli

BACKGROUND: Activators of Nitrile hydratase (NHase) are essential for functional NHase biosynthesis. However, the activator P14K in P. putida is difficult to heterogeneously express, which retards the clarification of the mechanism of P14K involved in the maturation of NHase. Although a strep tag co...

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Detalles Bibliográficos
Autores principales:	Liu, Yi, Cui, Wenjing, Fang, Yueqin, Yu, Yuechun, Cui, Youtian, Xia, Yuanyuan, Kobayashi, Michihiko, Zhou, Zhemin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3680314/ https://www.ncbi.nlm.nih.gov/pubmed/23731949 http://dx.doi.org/10.1186/1472-6750-13-48

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