Cargando…

Pif1 family helicases suppress genome instability at G-quadruplex motifs

The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. We show that exceptionally potent G-quadruplex unwinding is conserved amongst Pif1 helicases. Moreover, Pif1 helicases from organisms separated by >3...

Descripción completa

Detalles Bibliográficos
Autores principales: Paeschke, Katrin, Bochman, Matthew L., Garcia, P. Daniela, Cejka, Petr, Friedman, Katherine L., Kowalczykowski, Stephen C., Zakian, Virginia A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3680789/
https://www.ncbi.nlm.nih.gov/pubmed/23657261
http://dx.doi.org/10.1038/nature12149
_version_ 1782273156066574336
author Paeschke, Katrin
Bochman, Matthew L.
Garcia, P. Daniela
Cejka, Petr
Friedman, Katherine L.
Kowalczykowski, Stephen C.
Zakian, Virginia A.
author_facet Paeschke, Katrin
Bochman, Matthew L.
Garcia, P. Daniela
Cejka, Petr
Friedman, Katherine L.
Kowalczykowski, Stephen C.
Zakian, Virginia A.
author_sort Paeschke, Katrin
collection PubMed
description The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. We show that exceptionally potent G-quadruplex unwinding is conserved amongst Pif1 helicases. Moreover, Pif1 helicases from organisms separated by >3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to novel genetic and epigenetic changes. Further, when expressed in yeast, human Pif1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.
format Online
Article
Text
id pubmed-3680789
institution National Center for Biotechnology Information
language English
publishDate 2013
record_format MEDLINE/PubMed
spelling pubmed-36807892013-11-23 Pif1 family helicases suppress genome instability at G-quadruplex motifs Paeschke, Katrin Bochman, Matthew L. Garcia, P. Daniela Cejka, Petr Friedman, Katherine L. Kowalczykowski, Stephen C. Zakian, Virginia A. Nature Article The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. We show that exceptionally potent G-quadruplex unwinding is conserved amongst Pif1 helicases. Moreover, Pif1 helicases from organisms separated by >3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to novel genetic and epigenetic changes. Further, when expressed in yeast, human Pif1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening. 2013-05-08 2013-05-23 /pmc/articles/PMC3680789/ /pubmed/23657261 http://dx.doi.org/10.1038/nature12149 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Paeschke, Katrin
Bochman, Matthew L.
Garcia, P. Daniela
Cejka, Petr
Friedman, Katherine L.
Kowalczykowski, Stephen C.
Zakian, Virginia A.
Pif1 family helicases suppress genome instability at G-quadruplex motifs
title Pif1 family helicases suppress genome instability at G-quadruplex motifs
title_full Pif1 family helicases suppress genome instability at G-quadruplex motifs
title_fullStr Pif1 family helicases suppress genome instability at G-quadruplex motifs
title_full_unstemmed Pif1 family helicases suppress genome instability at G-quadruplex motifs
title_short Pif1 family helicases suppress genome instability at G-quadruplex motifs
title_sort pif1 family helicases suppress genome instability at g-quadruplex motifs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3680789/
https://www.ncbi.nlm.nih.gov/pubmed/23657261
http://dx.doi.org/10.1038/nature12149
work_keys_str_mv AT paeschkekatrin pif1familyhelicasessuppressgenomeinstabilityatgquadruplexmotifs
AT bochmanmatthewl pif1familyhelicasessuppressgenomeinstabilityatgquadruplexmotifs
AT garciapdaniela pif1familyhelicasessuppressgenomeinstabilityatgquadruplexmotifs
AT cejkapetr pif1familyhelicasessuppressgenomeinstabilityatgquadruplexmotifs
AT friedmankatherinel pif1familyhelicasessuppressgenomeinstabilityatgquadruplexmotifs
AT kowalczykowskistephenc pif1familyhelicasessuppressgenomeinstabilityatgquadruplexmotifs
AT zakianvirginiaa pif1familyhelicasessuppressgenomeinstabilityatgquadruplexmotifs