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Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation
Aim. To analyze the risks of preoperatively produced donor-specific antibody (DSA) in liver transplantation. Methods. DSA was assessed using direct complement-dependent cytotoxicity (CDC) and anti-human globulin- (AHG-) CDC tests, as well as the Luminex Single Antigen assay. Among 616 patients under...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681225/ https://www.ncbi.nlm.nih.gov/pubmed/23818917 http://dx.doi.org/10.1155/2013/972705 |
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author | Yoshizawa, Atsushi Egawa, Hiroto Yurugi, Kimiko Hishida, Rie Tsuji, Hiroaki Ashihara, Eiji Miyagawa-Hayashino, Aya Teramukai, Satoshi Maekawa, Taira Haga, Hironori Uemoto, Sinji |
author_facet | Yoshizawa, Atsushi Egawa, Hiroto Yurugi, Kimiko Hishida, Rie Tsuji, Hiroaki Ashihara, Eiji Miyagawa-Hayashino, Aya Teramukai, Satoshi Maekawa, Taira Haga, Hironori Uemoto, Sinji |
author_sort | Yoshizawa, Atsushi |
collection | PubMed |
description | Aim. To analyze the risks of preoperatively produced donor-specific antibody (DSA) in liver transplantation. Methods. DSA was assessed using direct complement-dependent cytotoxicity (CDC) and anti-human globulin- (AHG-) CDC tests, as well as the Luminex Single Antigen assay. Among 616 patients undergoing blood type identical or compatible living donor liver transplantation (LDLT), 21 patients were positive for CDC or AHG-CDC tests, and the preserved serum from 18 patients was examined to determine targeted Class I and II antigens. The relationships between the mean fluorescence intensity (MFI) of DSA and the clinical outcomes were analyzed. Results. Patients were divided into 3 groups according to the MFI of anti-Class I DSA: high (11 patients with MFI > 10,000), low (2 patients with MFI < 10,000), and negative (5 patients) MFI groups. Six of 11 patients with high Class-I DSA showed positive Class-II DSA. Hospital death occurred in 7 patients of the high MFI group. High MFI was a significant risk factor for mortality (P = 0.0155). Univariate analysis showed a significant correlation between MFI strength and C4d deposition (P = 0.0498). Conclusions. HLA Class I DSA with MFI > 10,000 had a significant negative effect on the clinical outcome of patients with preformed DSA in LDLT. |
format | Online Article Text |
id | pubmed-3681225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36812252013-07-01 Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation Yoshizawa, Atsushi Egawa, Hiroto Yurugi, Kimiko Hishida, Rie Tsuji, Hiroaki Ashihara, Eiji Miyagawa-Hayashino, Aya Teramukai, Satoshi Maekawa, Taira Haga, Hironori Uemoto, Sinji Clin Dev Immunol Clinical Study Aim. To analyze the risks of preoperatively produced donor-specific antibody (DSA) in liver transplantation. Methods. DSA was assessed using direct complement-dependent cytotoxicity (CDC) and anti-human globulin- (AHG-) CDC tests, as well as the Luminex Single Antigen assay. Among 616 patients undergoing blood type identical or compatible living donor liver transplantation (LDLT), 21 patients were positive for CDC or AHG-CDC tests, and the preserved serum from 18 patients was examined to determine targeted Class I and II antigens. The relationships between the mean fluorescence intensity (MFI) of DSA and the clinical outcomes were analyzed. Results. Patients were divided into 3 groups according to the MFI of anti-Class I DSA: high (11 patients with MFI > 10,000), low (2 patients with MFI < 10,000), and negative (5 patients) MFI groups. Six of 11 patients with high Class-I DSA showed positive Class-II DSA. Hospital death occurred in 7 patients of the high MFI group. High MFI was a significant risk factor for mortality (P = 0.0155). Univariate analysis showed a significant correlation between MFI strength and C4d deposition (P = 0.0498). Conclusions. HLA Class I DSA with MFI > 10,000 had a significant negative effect on the clinical outcome of patients with preformed DSA in LDLT. Hindawi Publishing Corporation 2013 2013-05-29 /pmc/articles/PMC3681225/ /pubmed/23818917 http://dx.doi.org/10.1155/2013/972705 Text en Copyright © 2013 Atsushi Yoshizawa et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Study Yoshizawa, Atsushi Egawa, Hiroto Yurugi, Kimiko Hishida, Rie Tsuji, Hiroaki Ashihara, Eiji Miyagawa-Hayashino, Aya Teramukai, Satoshi Maekawa, Taira Haga, Hironori Uemoto, Sinji Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation |
title | Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation |
title_full | Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation |
title_fullStr | Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation |
title_full_unstemmed | Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation |
title_short | Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation |
title_sort | significance of semiquantitative assessment of preformed donor-specific antibody using luminex single bead assay in living related liver transplantation |
topic | Clinical Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681225/ https://www.ncbi.nlm.nih.gov/pubmed/23818917 http://dx.doi.org/10.1155/2013/972705 |
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