Cargando…

Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation

Aim. To analyze the risks of preoperatively produced donor-specific antibody (DSA) in liver transplantation. Methods. DSA was assessed using direct complement-dependent cytotoxicity (CDC) and anti-human globulin- (AHG-) CDC tests, as well as the Luminex Single Antigen assay. Among 616 patients under...

Descripción completa

Detalles Bibliográficos
Autores principales: Yoshizawa, Atsushi, Egawa, Hiroto, Yurugi, Kimiko, Hishida, Rie, Tsuji, Hiroaki, Ashihara, Eiji, Miyagawa-Hayashino, Aya, Teramukai, Satoshi, Maekawa, Taira, Haga, Hironori, Uemoto, Sinji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681225/
https://www.ncbi.nlm.nih.gov/pubmed/23818917
http://dx.doi.org/10.1155/2013/972705
_version_ 1782273226376740864
author Yoshizawa, Atsushi
Egawa, Hiroto
Yurugi, Kimiko
Hishida, Rie
Tsuji, Hiroaki
Ashihara, Eiji
Miyagawa-Hayashino, Aya
Teramukai, Satoshi
Maekawa, Taira
Haga, Hironori
Uemoto, Sinji
author_facet Yoshizawa, Atsushi
Egawa, Hiroto
Yurugi, Kimiko
Hishida, Rie
Tsuji, Hiroaki
Ashihara, Eiji
Miyagawa-Hayashino, Aya
Teramukai, Satoshi
Maekawa, Taira
Haga, Hironori
Uemoto, Sinji
author_sort Yoshizawa, Atsushi
collection PubMed
description Aim. To analyze the risks of preoperatively produced donor-specific antibody (DSA) in liver transplantation. Methods. DSA was assessed using direct complement-dependent cytotoxicity (CDC) and anti-human globulin- (AHG-) CDC tests, as well as the Luminex Single Antigen assay. Among 616 patients undergoing blood type identical or compatible living donor liver transplantation (LDLT), 21 patients were positive for CDC or AHG-CDC tests, and the preserved serum from 18 patients was examined to determine targeted Class I and II antigens. The relationships between the mean fluorescence intensity (MFI) of DSA and the clinical outcomes were analyzed. Results. Patients were divided into 3 groups according to the MFI of anti-Class I DSA: high (11 patients with MFI > 10,000), low (2 patients with MFI < 10,000), and negative (5 patients) MFI groups. Six of 11 patients with high Class-I DSA showed positive Class-II DSA. Hospital death occurred in 7 patients of the high MFI group. High MFI was a significant risk factor for mortality (P = 0.0155). Univariate analysis showed a significant correlation between MFI strength and C4d deposition (P = 0.0498). Conclusions. HLA Class I DSA with MFI > 10,000 had a significant negative effect on the clinical outcome of patients with preformed DSA in LDLT.
format Online
Article
Text
id pubmed-3681225
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-36812252013-07-01 Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation Yoshizawa, Atsushi Egawa, Hiroto Yurugi, Kimiko Hishida, Rie Tsuji, Hiroaki Ashihara, Eiji Miyagawa-Hayashino, Aya Teramukai, Satoshi Maekawa, Taira Haga, Hironori Uemoto, Sinji Clin Dev Immunol Clinical Study Aim. To analyze the risks of preoperatively produced donor-specific antibody (DSA) in liver transplantation. Methods. DSA was assessed using direct complement-dependent cytotoxicity (CDC) and anti-human globulin- (AHG-) CDC tests, as well as the Luminex Single Antigen assay. Among 616 patients undergoing blood type identical or compatible living donor liver transplantation (LDLT), 21 patients were positive for CDC or AHG-CDC tests, and the preserved serum from 18 patients was examined to determine targeted Class I and II antigens. The relationships between the mean fluorescence intensity (MFI) of DSA and the clinical outcomes were analyzed. Results. Patients were divided into 3 groups according to the MFI of anti-Class I DSA: high (11 patients with MFI > 10,000), low (2 patients with MFI < 10,000), and negative (5 patients) MFI groups. Six of 11 patients with high Class-I DSA showed positive Class-II DSA. Hospital death occurred in 7 patients of the high MFI group. High MFI was a significant risk factor for mortality (P = 0.0155). Univariate analysis showed a significant correlation between MFI strength and C4d deposition (P = 0.0498). Conclusions. HLA Class I DSA with MFI > 10,000 had a significant negative effect on the clinical outcome of patients with preformed DSA in LDLT. Hindawi Publishing Corporation 2013 2013-05-29 /pmc/articles/PMC3681225/ /pubmed/23818917 http://dx.doi.org/10.1155/2013/972705 Text en Copyright © 2013 Atsushi Yoshizawa et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Study
Yoshizawa, Atsushi
Egawa, Hiroto
Yurugi, Kimiko
Hishida, Rie
Tsuji, Hiroaki
Ashihara, Eiji
Miyagawa-Hayashino, Aya
Teramukai, Satoshi
Maekawa, Taira
Haga, Hironori
Uemoto, Sinji
Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation
title Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation
title_full Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation
title_fullStr Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation
title_full_unstemmed Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation
title_short Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation
title_sort significance of semiquantitative assessment of preformed donor-specific antibody using luminex single bead assay in living related liver transplantation
topic Clinical Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681225/
https://www.ncbi.nlm.nih.gov/pubmed/23818917
http://dx.doi.org/10.1155/2013/972705
work_keys_str_mv AT yoshizawaatsushi significanceofsemiquantitativeassessmentofpreformeddonorspecificantibodyusingluminexsinglebeadassayinlivingrelatedlivertransplantation
AT egawahiroto significanceofsemiquantitativeassessmentofpreformeddonorspecificantibodyusingluminexsinglebeadassayinlivingrelatedlivertransplantation
AT yurugikimiko significanceofsemiquantitativeassessmentofpreformeddonorspecificantibodyusingluminexsinglebeadassayinlivingrelatedlivertransplantation
AT hishidarie significanceofsemiquantitativeassessmentofpreformeddonorspecificantibodyusingluminexsinglebeadassayinlivingrelatedlivertransplantation
AT tsujihiroaki significanceofsemiquantitativeassessmentofpreformeddonorspecificantibodyusingluminexsinglebeadassayinlivingrelatedlivertransplantation
AT ashiharaeiji significanceofsemiquantitativeassessmentofpreformeddonorspecificantibodyusingluminexsinglebeadassayinlivingrelatedlivertransplantation
AT miyagawahayashinoaya significanceofsemiquantitativeassessmentofpreformeddonorspecificantibodyusingluminexsinglebeadassayinlivingrelatedlivertransplantation
AT teramukaisatoshi significanceofsemiquantitativeassessmentofpreformeddonorspecificantibodyusingluminexsinglebeadassayinlivingrelatedlivertransplantation
AT maekawataira significanceofsemiquantitativeassessmentofpreformeddonorspecificantibodyusingluminexsinglebeadassayinlivingrelatedlivertransplantation
AT hagahironori significanceofsemiquantitativeassessmentofpreformeddonorspecificantibodyusingluminexsinglebeadassayinlivingrelatedlivertransplantation
AT uemotosinji significanceofsemiquantitativeassessmentofpreformeddonorspecificantibodyusingluminexsinglebeadassayinlivingrelatedlivertransplantation