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FoxO6 and PGC-1α form a regulatory loop in myogenic cells

Transcription factors of the FoxO (forkhead box O) family regulate a wide range of cellular physiological processes, including metabolic adaptation and myogenic differentiation. The transcriptional activity of most FoxO members is inhibitory to myogenic differentiation and overexpression of FoxO1 in...

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Autores principales: Chung, Shih Ying, Huang, Wei Chieh, Su, Ching Wen, Lee, Kuan Wei, Chi, Hsiang Cheng, Lin, Cheng Tao, Chen, Szu-Tah, Huang, Kai Min, Tsai, Mu Shiun, Yu, Hui Peng, Chen, Shen Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681252/
https://www.ncbi.nlm.nih.gov/pubmed/23639108
http://dx.doi.org/10.1042/BSR20130031
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author Chung, Shih Ying
Huang, Wei Chieh
Su, Ching Wen
Lee, Kuan Wei
Chi, Hsiang Cheng
Lin, Cheng Tao
Chen, Szu-Tah
Huang, Kai Min
Tsai, Mu Shiun
Yu, Hui Peng
Chen, Shen Liang
author_facet Chung, Shih Ying
Huang, Wei Chieh
Su, Ching Wen
Lee, Kuan Wei
Chi, Hsiang Cheng
Lin, Cheng Tao
Chen, Szu-Tah
Huang, Kai Min
Tsai, Mu Shiun
Yu, Hui Peng
Chen, Shen Liang
author_sort Chung, Shih Ying
collection PubMed
description Transcription factors of the FoxO (forkhead box O) family regulate a wide range of cellular physiological processes, including metabolic adaptation and myogenic differentiation. The transcriptional activity of most FoxO members is inhibitory to myogenic differentiation and overexpression of FoxO1 inhibits the development of oxidative type I fibres in vivo. In this study, we found that FoxO6, the last discovered FoxO family member, is expressed ubiquitously in various tissues but with higher expression levels in oxidative tissues, such as brain and oxidative muscles. Both the expression level and promoter activity of FoxO6 were found to be enhanced by PGC-1α (peroxisome-proliferator-activated receptor γ co-activator 1α), thus explained its enriched expression in oxidative tissues. We further demonstrated that FoxO6 represses the expression of PGC-1α via direct binding to an upstream A/T-rich element (AAGATATCAAAACA,−2228–2215) in the PGC-1α promoter. Oxidative low-intensity exercise induced PGC-1α but reduced FoxO6 expression levels in hind leg muscles, and the binding of FoxO6 to PGC-1α promoter was also prevented by exercise. As FoxO6 promoter can be co-activated by PGC-1α and its promoter in turn can be repressed by FoxO6, it suggests that FoxO6 and PGC-1α form a regulatory loop for setting oxidative metabolism level in the skeletal muscle, which can be entrained by exercise.
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spelling pubmed-36812522013-06-17 FoxO6 and PGC-1α form a regulatory loop in myogenic cells Chung, Shih Ying Huang, Wei Chieh Su, Ching Wen Lee, Kuan Wei Chi, Hsiang Cheng Lin, Cheng Tao Chen, Szu-Tah Huang, Kai Min Tsai, Mu Shiun Yu, Hui Peng Chen, Shen Liang Biosci Rep Original Paper Transcription factors of the FoxO (forkhead box O) family regulate a wide range of cellular physiological processes, including metabolic adaptation and myogenic differentiation. The transcriptional activity of most FoxO members is inhibitory to myogenic differentiation and overexpression of FoxO1 inhibits the development of oxidative type I fibres in vivo. In this study, we found that FoxO6, the last discovered FoxO family member, is expressed ubiquitously in various tissues but with higher expression levels in oxidative tissues, such as brain and oxidative muscles. Both the expression level and promoter activity of FoxO6 were found to be enhanced by PGC-1α (peroxisome-proliferator-activated receptor γ co-activator 1α), thus explained its enriched expression in oxidative tissues. We further demonstrated that FoxO6 represses the expression of PGC-1α via direct binding to an upstream A/T-rich element (AAGATATCAAAACA,−2228–2215) in the PGC-1α promoter. Oxidative low-intensity exercise induced PGC-1α but reduced FoxO6 expression levels in hind leg muscles, and the binding of FoxO6 to PGC-1α promoter was also prevented by exercise. As FoxO6 promoter can be co-activated by PGC-1α and its promoter in turn can be repressed by FoxO6, it suggests that FoxO6 and PGC-1α form a regulatory loop for setting oxidative metabolism level in the skeletal muscle, which can be entrained by exercise. Portland Press Ltd. 2013-06-13 /pmc/articles/PMC3681252/ /pubmed/23639108 http://dx.doi.org/10.1042/BSR20130031 Text en © 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Paper
Chung, Shih Ying
Huang, Wei Chieh
Su, Ching Wen
Lee, Kuan Wei
Chi, Hsiang Cheng
Lin, Cheng Tao
Chen, Szu-Tah
Huang, Kai Min
Tsai, Mu Shiun
Yu, Hui Peng
Chen, Shen Liang
FoxO6 and PGC-1α form a regulatory loop in myogenic cells
title FoxO6 and PGC-1α form a regulatory loop in myogenic cells
title_full FoxO6 and PGC-1α form a regulatory loop in myogenic cells
title_fullStr FoxO6 and PGC-1α form a regulatory loop in myogenic cells
title_full_unstemmed FoxO6 and PGC-1α form a regulatory loop in myogenic cells
title_short FoxO6 and PGC-1α form a regulatory loop in myogenic cells
title_sort foxo6 and pgc-1α form a regulatory loop in myogenic cells
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681252/
https://www.ncbi.nlm.nih.gov/pubmed/23639108
http://dx.doi.org/10.1042/BSR20130031
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