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Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia

The purpose of this study is to investigate the possible different cellular marker expression associated with spinal cord microglial activation in different pain models. Immunohistochemistry and western blotting analysis of CD45, CD68, and MHC class I antigen as well as CD11b and Iba-1 in the spinal...

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Detalles Bibliográficos
Autores principales: Li, Kai, Tan, Yong-Hui, Light, Alan R., Fu, Kai-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681311/
https://www.ncbi.nlm.nih.gov/pubmed/23818916
http://dx.doi.org/10.1155/2013/901420
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author Li, Kai
Tan, Yong-Hui
Light, Alan R.
Fu, Kai-Yuan
author_facet Li, Kai
Tan, Yong-Hui
Light, Alan R.
Fu, Kai-Yuan
author_sort Li, Kai
collection PubMed
description The purpose of this study is to investigate the possible different cellular marker expression associated with spinal cord microglial activation in different pain models. Immunohistochemistry and western blotting analysis of CD45, CD68, and MHC class I antigen as well as CD11b and Iba-1 in the spinal cord were quantitatively compared among widely used three pain animal models, complete Freund's adjuvant (CFA) injection, formalin injection, and chronic constriction injury (CCI) models. The results showed that significant upregulated expressions of CD45 and MHC class I antigen in spinal microglia as well as morphological changes with increased staining with CD11b and Iba-1 were seen in CCI and formalin models and not found in CFA-induced inflammatory pain model. CD68 expression was only detected in CCI model. Our findings suggested that different peripheral tissue injuries produced differential phenotypic changes associated with spinal microglial activation; peripheral nerve injury might induce spinal microglia to acquire these immunomolecular phenotypic changes.
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spelling pubmed-36813112013-07-01 Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia Li, Kai Tan, Yong-Hui Light, Alan R. Fu, Kai-Yuan Clin Dev Immunol Research Article The purpose of this study is to investigate the possible different cellular marker expression associated with spinal cord microglial activation in different pain models. Immunohistochemistry and western blotting analysis of CD45, CD68, and MHC class I antigen as well as CD11b and Iba-1 in the spinal cord were quantitatively compared among widely used three pain animal models, complete Freund's adjuvant (CFA) injection, formalin injection, and chronic constriction injury (CCI) models. The results showed that significant upregulated expressions of CD45 and MHC class I antigen in spinal microglia as well as morphological changes with increased staining with CD11b and Iba-1 were seen in CCI and formalin models and not found in CFA-induced inflammatory pain model. CD68 expression was only detected in CCI model. Our findings suggested that different peripheral tissue injuries produced differential phenotypic changes associated with spinal microglial activation; peripheral nerve injury might induce spinal microglia to acquire these immunomolecular phenotypic changes. Hindawi Publishing Corporation 2013 2013-05-29 /pmc/articles/PMC3681311/ /pubmed/23818916 http://dx.doi.org/10.1155/2013/901420 Text en Copyright © 2013 Kai Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Kai
Tan, Yong-Hui
Light, Alan R.
Fu, Kai-Yuan
Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia
title Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia
title_full Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia
title_fullStr Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia
title_full_unstemmed Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia
title_short Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia
title_sort different peripheral tissue injury induces differential phenotypic changes of spinal activated microglia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681311/
https://www.ncbi.nlm.nih.gov/pubmed/23818916
http://dx.doi.org/10.1155/2013/901420
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