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Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia
The purpose of this study is to investigate the possible different cellular marker expression associated with spinal cord microglial activation in different pain models. Immunohistochemistry and western blotting analysis of CD45, CD68, and MHC class I antigen as well as CD11b and Iba-1 in the spinal...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681311/ https://www.ncbi.nlm.nih.gov/pubmed/23818916 http://dx.doi.org/10.1155/2013/901420 |
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author | Li, Kai Tan, Yong-Hui Light, Alan R. Fu, Kai-Yuan |
author_facet | Li, Kai Tan, Yong-Hui Light, Alan R. Fu, Kai-Yuan |
author_sort | Li, Kai |
collection | PubMed |
description | The purpose of this study is to investigate the possible different cellular marker expression associated with spinal cord microglial activation in different pain models. Immunohistochemistry and western blotting analysis of CD45, CD68, and MHC class I antigen as well as CD11b and Iba-1 in the spinal cord were quantitatively compared among widely used three pain animal models, complete Freund's adjuvant (CFA) injection, formalin injection, and chronic constriction injury (CCI) models. The results showed that significant upregulated expressions of CD45 and MHC class I antigen in spinal microglia as well as morphological changes with increased staining with CD11b and Iba-1 were seen in CCI and formalin models and not found in CFA-induced inflammatory pain model. CD68 expression was only detected in CCI model. Our findings suggested that different peripheral tissue injuries produced differential phenotypic changes associated with spinal microglial activation; peripheral nerve injury might induce spinal microglia to acquire these immunomolecular phenotypic changes. |
format | Online Article Text |
id | pubmed-3681311 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36813112013-07-01 Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia Li, Kai Tan, Yong-Hui Light, Alan R. Fu, Kai-Yuan Clin Dev Immunol Research Article The purpose of this study is to investigate the possible different cellular marker expression associated with spinal cord microglial activation in different pain models. Immunohistochemistry and western blotting analysis of CD45, CD68, and MHC class I antigen as well as CD11b and Iba-1 in the spinal cord were quantitatively compared among widely used three pain animal models, complete Freund's adjuvant (CFA) injection, formalin injection, and chronic constriction injury (CCI) models. The results showed that significant upregulated expressions of CD45 and MHC class I antigen in spinal microglia as well as morphological changes with increased staining with CD11b and Iba-1 were seen in CCI and formalin models and not found in CFA-induced inflammatory pain model. CD68 expression was only detected in CCI model. Our findings suggested that different peripheral tissue injuries produced differential phenotypic changes associated with spinal microglial activation; peripheral nerve injury might induce spinal microglia to acquire these immunomolecular phenotypic changes. Hindawi Publishing Corporation 2013 2013-05-29 /pmc/articles/PMC3681311/ /pubmed/23818916 http://dx.doi.org/10.1155/2013/901420 Text en Copyright © 2013 Kai Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Li, Kai Tan, Yong-Hui Light, Alan R. Fu, Kai-Yuan Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia |
title | Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia |
title_full | Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia |
title_fullStr | Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia |
title_full_unstemmed | Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia |
title_short | Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia |
title_sort | different peripheral tissue injury induces differential phenotypic changes of spinal activated microglia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681311/ https://www.ncbi.nlm.nih.gov/pubmed/23818916 http://dx.doi.org/10.1155/2013/901420 |
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