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MAP65/Ase1 promote microtubule flexibility

Microtubules (MTs) are dynamic cytoskeletal elements involved in numerous cellular processes. Although they are highly rigid polymers with a persistence length of 1–8 mm, they may exhibit a curved shape at a scale of few micrometers within cells, depending on their biological functions. However, how...

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Autores principales: Portran, D., Zoccoler, M., Gaillard, J., Stoppin-Mellet, V., Neumann, E., Arnal, I., Martiel, J. L., Vantard, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681700/
https://www.ncbi.nlm.nih.gov/pubmed/23615441
http://dx.doi.org/10.1091/mbc.E13-03-0141
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author Portran, D.
Zoccoler, M.
Gaillard, J.
Stoppin-Mellet, V.
Neumann, E.
Arnal, I.
Martiel, J. L.
Vantard, M.
author_facet Portran, D.
Zoccoler, M.
Gaillard, J.
Stoppin-Mellet, V.
Neumann, E.
Arnal, I.
Martiel, J. L.
Vantard, M.
author_sort Portran, D.
collection PubMed
description Microtubules (MTs) are dynamic cytoskeletal elements involved in numerous cellular processes. Although they are highly rigid polymers with a persistence length of 1–8 mm, they may exhibit a curved shape at a scale of few micrometers within cells, depending on their biological functions. However, how MT flexural rigidity in cells is regulated remains poorly understood. Here we ask whether MT-associated proteins (MAPs) could locally control the mechanical properties of MTs. We show that two major cross-linkers of the conserved MAP65/PRC1/Ase1 family drastically decrease MT rigidity. Their MT-binding domain mediates this effect. Remarkably, the softening effect of MAP65 observed on single MTs is maintained when MTs are cross-linked. By reconstituting physical collisions between growing MTs/MT bundles, we further show that the decrease in MT stiffness induced by MAP65 proteins is responsible for the sharp bending deformations observed in cells when they coalign at a steep angle to create bundles. Taken together, these data provide new insights into how MAP65, by modifying MT mechanical properties, may regulate the formation of complex MT arrays.
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spelling pubmed-36817002013-08-30 MAP65/Ase1 promote microtubule flexibility Portran, D. Zoccoler, M. Gaillard, J. Stoppin-Mellet, V. Neumann, E. Arnal, I. Martiel, J. L. Vantard, M. Mol Biol Cell Articles Microtubules (MTs) are dynamic cytoskeletal elements involved in numerous cellular processes. Although they are highly rigid polymers with a persistence length of 1–8 mm, they may exhibit a curved shape at a scale of few micrometers within cells, depending on their biological functions. However, how MT flexural rigidity in cells is regulated remains poorly understood. Here we ask whether MT-associated proteins (MAPs) could locally control the mechanical properties of MTs. We show that two major cross-linkers of the conserved MAP65/PRC1/Ase1 family drastically decrease MT rigidity. Their MT-binding domain mediates this effect. Remarkably, the softening effect of MAP65 observed on single MTs is maintained when MTs are cross-linked. By reconstituting physical collisions between growing MTs/MT bundles, we further show that the decrease in MT stiffness induced by MAP65 proteins is responsible for the sharp bending deformations observed in cells when they coalign at a steep angle to create bundles. Taken together, these data provide new insights into how MAP65, by modifying MT mechanical properties, may regulate the formation of complex MT arrays. The American Society for Cell Biology 2013-06-15 /pmc/articles/PMC3681700/ /pubmed/23615441 http://dx.doi.org/10.1091/mbc.E13-03-0141 Text en © 2013 Portran et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Portran, D.
Zoccoler, M.
Gaillard, J.
Stoppin-Mellet, V.
Neumann, E.
Arnal, I.
Martiel, J. L.
Vantard, M.
MAP65/Ase1 promote microtubule flexibility
title MAP65/Ase1 promote microtubule flexibility
title_full MAP65/Ase1 promote microtubule flexibility
title_fullStr MAP65/Ase1 promote microtubule flexibility
title_full_unstemmed MAP65/Ase1 promote microtubule flexibility
title_short MAP65/Ase1 promote microtubule flexibility
title_sort map65/ase1 promote microtubule flexibility
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681700/
https://www.ncbi.nlm.nih.gov/pubmed/23615441
http://dx.doi.org/10.1091/mbc.E13-03-0141
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