Cargando…

Substrate Topography Determines Neuronal Polarization and Growth In Vitro

The establishment of neuronal connectivity depends on the correct initial polarization of the young neurons. In vivo, developing neurons sense a multitude of inputs and a great number of molecules are described that affect their outgrowth. In vitro, many studies have shown the possibility to influen...

Descripción completa

Detalles Bibliográficos
Autores principales: Micholt, Liesbeth, Gärtner, Annette, Prodanov, Dimiter, Braeken, Dries, Dotti, Carlos G., Bartic, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681759/
https://www.ncbi.nlm.nih.gov/pubmed/23785482
http://dx.doi.org/10.1371/journal.pone.0066170
_version_ 1782273316455710720
author Micholt, Liesbeth
Gärtner, Annette
Prodanov, Dimiter
Braeken, Dries
Dotti, Carlos G.
Bartic, Carmen
author_facet Micholt, Liesbeth
Gärtner, Annette
Prodanov, Dimiter
Braeken, Dries
Dotti, Carlos G.
Bartic, Carmen
author_sort Micholt, Liesbeth
collection PubMed
description The establishment of neuronal connectivity depends on the correct initial polarization of the young neurons. In vivo, developing neurons sense a multitude of inputs and a great number of molecules are described that affect their outgrowth. In vitro, many studies have shown the possibility to influence neuronal morphology and growth by biophysical, i.e. topographic, signaling. In this work we have taken this approach one step further and investigated the impact of substrate topography in the very early differentiation stages of developing neurons, i.e. when the cell is still at the round stage and when the first neurite is forming. For this purpose we fabricated micron sized pillar structures with highly reproducible feature sizes, and analyzed neurons on the interface of flat and topographic surfaces. We found that topographic signaling was able to attract the polarization markers of mouse embryonic neurons -N-cadherin, Golgi-centrosome complex and the first bud were oriented towards topographic stimuli. Consecutively, the axon was also preferentially extending along the pillars. These events seemed to occur regardless of pillar dimensions in the range we examined. However, we found differences in neurite length that depended on pillar dimensions. This study is one of the first to describe in detail the very early response of hippocampal neurons to topographic stimuli.
format Online
Article
Text
id pubmed-3681759
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-36817592013-06-19 Substrate Topography Determines Neuronal Polarization and Growth In Vitro Micholt, Liesbeth Gärtner, Annette Prodanov, Dimiter Braeken, Dries Dotti, Carlos G. Bartic, Carmen PLoS One Research Article The establishment of neuronal connectivity depends on the correct initial polarization of the young neurons. In vivo, developing neurons sense a multitude of inputs and a great number of molecules are described that affect their outgrowth. In vitro, many studies have shown the possibility to influence neuronal morphology and growth by biophysical, i.e. topographic, signaling. In this work we have taken this approach one step further and investigated the impact of substrate topography in the very early differentiation stages of developing neurons, i.e. when the cell is still at the round stage and when the first neurite is forming. For this purpose we fabricated micron sized pillar structures with highly reproducible feature sizes, and analyzed neurons on the interface of flat and topographic surfaces. We found that topographic signaling was able to attract the polarization markers of mouse embryonic neurons -N-cadherin, Golgi-centrosome complex and the first bud were oriented towards topographic stimuli. Consecutively, the axon was also preferentially extending along the pillars. These events seemed to occur regardless of pillar dimensions in the range we examined. However, we found differences in neurite length that depended on pillar dimensions. This study is one of the first to describe in detail the very early response of hippocampal neurons to topographic stimuli. Public Library of Science 2013-06-13 /pmc/articles/PMC3681759/ /pubmed/23785482 http://dx.doi.org/10.1371/journal.pone.0066170 Text en © 2013 Micholt et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Micholt, Liesbeth
Gärtner, Annette
Prodanov, Dimiter
Braeken, Dries
Dotti, Carlos G.
Bartic, Carmen
Substrate Topography Determines Neuronal Polarization and Growth In Vitro
title Substrate Topography Determines Neuronal Polarization and Growth In Vitro
title_full Substrate Topography Determines Neuronal Polarization and Growth In Vitro
title_fullStr Substrate Topography Determines Neuronal Polarization and Growth In Vitro
title_full_unstemmed Substrate Topography Determines Neuronal Polarization and Growth In Vitro
title_short Substrate Topography Determines Neuronal Polarization and Growth In Vitro
title_sort substrate topography determines neuronal polarization and growth in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681759/
https://www.ncbi.nlm.nih.gov/pubmed/23785482
http://dx.doi.org/10.1371/journal.pone.0066170
work_keys_str_mv AT micholtliesbeth substratetopographydeterminesneuronalpolarizationandgrowthinvitro
AT gartnerannette substratetopographydeterminesneuronalpolarizationandgrowthinvitro
AT prodanovdimiter substratetopographydeterminesneuronalpolarizationandgrowthinvitro
AT braekendries substratetopographydeterminesneuronalpolarizationandgrowthinvitro
AT dotticarlosg substratetopographydeterminesneuronalpolarizationandgrowthinvitro
AT barticcarmen substratetopographydeterminesneuronalpolarizationandgrowthinvitro