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Substrate Topography Determines Neuronal Polarization and Growth In Vitro
The establishment of neuronal connectivity depends on the correct initial polarization of the young neurons. In vivo, developing neurons sense a multitude of inputs and a great number of molecules are described that affect their outgrowth. In vitro, many studies have shown the possibility to influen...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681759/ https://www.ncbi.nlm.nih.gov/pubmed/23785482 http://dx.doi.org/10.1371/journal.pone.0066170 |
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author | Micholt, Liesbeth Gärtner, Annette Prodanov, Dimiter Braeken, Dries Dotti, Carlos G. Bartic, Carmen |
author_facet | Micholt, Liesbeth Gärtner, Annette Prodanov, Dimiter Braeken, Dries Dotti, Carlos G. Bartic, Carmen |
author_sort | Micholt, Liesbeth |
collection | PubMed |
description | The establishment of neuronal connectivity depends on the correct initial polarization of the young neurons. In vivo, developing neurons sense a multitude of inputs and a great number of molecules are described that affect their outgrowth. In vitro, many studies have shown the possibility to influence neuronal morphology and growth by biophysical, i.e. topographic, signaling. In this work we have taken this approach one step further and investigated the impact of substrate topography in the very early differentiation stages of developing neurons, i.e. when the cell is still at the round stage and when the first neurite is forming. For this purpose we fabricated micron sized pillar structures with highly reproducible feature sizes, and analyzed neurons on the interface of flat and topographic surfaces. We found that topographic signaling was able to attract the polarization markers of mouse embryonic neurons -N-cadherin, Golgi-centrosome complex and the first bud were oriented towards topographic stimuli. Consecutively, the axon was also preferentially extending along the pillars. These events seemed to occur regardless of pillar dimensions in the range we examined. However, we found differences in neurite length that depended on pillar dimensions. This study is one of the first to describe in detail the very early response of hippocampal neurons to topographic stimuli. |
format | Online Article Text |
id | pubmed-3681759 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36817592013-06-19 Substrate Topography Determines Neuronal Polarization and Growth In Vitro Micholt, Liesbeth Gärtner, Annette Prodanov, Dimiter Braeken, Dries Dotti, Carlos G. Bartic, Carmen PLoS One Research Article The establishment of neuronal connectivity depends on the correct initial polarization of the young neurons. In vivo, developing neurons sense a multitude of inputs and a great number of molecules are described that affect their outgrowth. In vitro, many studies have shown the possibility to influence neuronal morphology and growth by biophysical, i.e. topographic, signaling. In this work we have taken this approach one step further and investigated the impact of substrate topography in the very early differentiation stages of developing neurons, i.e. when the cell is still at the round stage and when the first neurite is forming. For this purpose we fabricated micron sized pillar structures with highly reproducible feature sizes, and analyzed neurons on the interface of flat and topographic surfaces. We found that topographic signaling was able to attract the polarization markers of mouse embryonic neurons -N-cadherin, Golgi-centrosome complex and the first bud were oriented towards topographic stimuli. Consecutively, the axon was also preferentially extending along the pillars. These events seemed to occur regardless of pillar dimensions in the range we examined. However, we found differences in neurite length that depended on pillar dimensions. This study is one of the first to describe in detail the very early response of hippocampal neurons to topographic stimuli. Public Library of Science 2013-06-13 /pmc/articles/PMC3681759/ /pubmed/23785482 http://dx.doi.org/10.1371/journal.pone.0066170 Text en © 2013 Micholt et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Micholt, Liesbeth Gärtner, Annette Prodanov, Dimiter Braeken, Dries Dotti, Carlos G. Bartic, Carmen Substrate Topography Determines Neuronal Polarization and Growth In Vitro |
title | Substrate Topography Determines Neuronal Polarization and Growth In Vitro
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title_full | Substrate Topography Determines Neuronal Polarization and Growth In Vitro
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title_fullStr | Substrate Topography Determines Neuronal Polarization and Growth In Vitro
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title_full_unstemmed | Substrate Topography Determines Neuronal Polarization and Growth In Vitro
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title_short | Substrate Topography Determines Neuronal Polarization and Growth In Vitro
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title_sort | substrate topography determines neuronal polarization and growth in vitro |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681759/ https://www.ncbi.nlm.nih.gov/pubmed/23785482 http://dx.doi.org/10.1371/journal.pone.0066170 |
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