TALEN-mediated editing of the Mouse Y Chromosome

The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and i...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Haoyi, Hu, Yueh-Chiang, Markoulaki, Styliani, Welstead, G. Grant, Cheng, Albert W., Shivalila, Chikdu S., Pyntikova, Tatyana, Dadon, Daniel B., Voytas, Daniel F., Bogdanove, Adam J., Page, David C., Jaenisch, Rudolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681814/
https://www.ncbi.nlm.nih.gov/pubmed/23666012
http://dx.doi.org/10.1038/nbt.2595
Descripción
Sumario:The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes – Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome.

Ejemplares similares