TALEN-mediated editing of the Mouse Y Chromosome

The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and i...

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Autores principales: Wang, Haoyi, Hu, Yueh-Chiang, Markoulaki, Styliani, Welstead, G. Grant, Cheng, Albert W., Shivalila, Chikdu S., Pyntikova, Tatyana, Dadon, Daniel B., Voytas, Daniel F., Bogdanove, Adam J., Page, David C., Jaenisch, Rudolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681814/
https://www.ncbi.nlm.nih.gov/pubmed/23666012
http://dx.doi.org/10.1038/nbt.2595
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author Wang, Haoyi
Hu, Yueh-Chiang
Markoulaki, Styliani
Welstead, G. Grant
Cheng, Albert W.
Shivalila, Chikdu S.
Pyntikova, Tatyana
Dadon, Daniel B.
Voytas, Daniel F.
Bogdanove, Adam J.
Page, David C.
Jaenisch, Rudolf
author_facet Wang, Haoyi
Hu, Yueh-Chiang
Markoulaki, Styliani
Welstead, G. Grant
Cheng, Albert W.
Shivalila, Chikdu S.
Pyntikova, Tatyana
Dadon, Daniel B.
Voytas, Daniel F.
Bogdanove, Adam J.
Page, David C.
Jaenisch, Rudolf
author_sort Wang, Haoyi
collection PubMed
description The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes – Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome.
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spelling pubmed-36818142013-12-01 TALEN-mediated editing of the Mouse Y Chromosome Wang, Haoyi Hu, Yueh-Chiang Markoulaki, Styliani Welstead, G. Grant Cheng, Albert W. Shivalila, Chikdu S. Pyntikova, Tatyana Dadon, Daniel B. Voytas, Daniel F. Bogdanove, Adam J. Page, David C. Jaenisch, Rudolf Nat Biotechnol Article The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes – Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome. 2013-05-12 2013-06 /pmc/articles/PMC3681814/ /pubmed/23666012 http://dx.doi.org/10.1038/nbt.2595 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wang, Haoyi
Hu, Yueh-Chiang
Markoulaki, Styliani
Welstead, G. Grant
Cheng, Albert W.
Shivalila, Chikdu S.
Pyntikova, Tatyana
Dadon, Daniel B.
Voytas, Daniel F.
Bogdanove, Adam J.
Page, David C.
Jaenisch, Rudolf
TALEN-mediated editing of the Mouse Y Chromosome
title TALEN-mediated editing of the Mouse Y Chromosome
title_full TALEN-mediated editing of the Mouse Y Chromosome
title_fullStr TALEN-mediated editing of the Mouse Y Chromosome
title_full_unstemmed TALEN-mediated editing of the Mouse Y Chromosome
title_short TALEN-mediated editing of the Mouse Y Chromosome
title_sort talen-mediated editing of the mouse y chromosome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681814/
https://www.ncbi.nlm.nih.gov/pubmed/23666012
http://dx.doi.org/10.1038/nbt.2595
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