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Engineering of Family-5 Glycoside Hydrolase (Cel5A) from an Uncultured Bacterium for Efficient Hydrolysis of Cellulosic Substrates
Cel5A, an endoglucanase, was derived from the metagenomic library of vermicompost. The deduced amino acid sequence of Cel5A shows high sequence homology with family-5 glycoside hydrolases, which contain a single catalytic domain but no distinct cellulose-binding domain. Random mutagenesis and cellul...
Autores principales: | Telke, Amar A., Zhuang, Ningning, Ghatge, Sunil S., Lee, Sook-Hee, Ali Shah, Asad, Khan, Haji, Um, Youngsoon, Shin, Hyun-Dong, Chung, Young Ryun, Lee, Kon Ho, Kim, Seon-Won |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681849/ https://www.ncbi.nlm.nih.gov/pubmed/23785445 http://dx.doi.org/10.1371/journal.pone.0065727 |
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