Cargando…
The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress
The control of mRNA biogenesis is exerted at several steps. In response to extracellular stimuli, stress-activated protein kinases (SAPK) modulate gene expression to maximize cell survival. In yeast, the Hog1 SAPK plays a key role in reprogramming the gene expression pattern required for cell surviv...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3682539/ https://www.ncbi.nlm.nih.gov/pubmed/23645671 http://dx.doi.org/10.1074/jbc.M112.444042 |
_version_ | 1782273394691014656 |
---|---|
author | Regot, Sergi de Nadal, Eulàlia Rodríguez-Navarro, Susana González-Novo, Alberto Pérez-Fernandez, Jorge Gadal, Olivier Seisenbacher, Gerhard Ammerer, Gustav Posas, Francesc |
author_facet | Regot, Sergi de Nadal, Eulàlia Rodríguez-Navarro, Susana González-Novo, Alberto Pérez-Fernandez, Jorge Gadal, Olivier Seisenbacher, Gerhard Ammerer, Gustav Posas, Francesc |
author_sort | Regot, Sergi |
collection | PubMed |
description | The control of mRNA biogenesis is exerted at several steps. In response to extracellular stimuli, stress-activated protein kinases (SAPK) modulate gene expression to maximize cell survival. In yeast, the Hog1 SAPK plays a key role in reprogramming the gene expression pattern required for cell survival upon osmostress by acting during transcriptional initiation and elongation. Here, we genetically show that an intact nuclear pore complex is important for cell survival and maximal expression of stress-responsive genes. The Hog1 SAPK associates with nuclear pore complex components and directly phosphorylates the Nup1, Nup2, and Nup60 components of the inner nuclear basket. Mutation of those factors resulted in a deficient export of stress-responsive genes upon stress. Association of Nup1, Nup2, and Nup60 to stress-responsive promoters occurs upon stress depending on Hog1 activity. Accordingly, STL1 gene territory is maintained at the nuclear periphery upon osmostress in a Hog1-dependent manner. Cells containing non-phosphorylatable mutants in Nup1 or Nup2 display reduced expression of stress-responsive genes. Together, proper mRNA biogenesis of stress-responsive genes requires of the coordinate action of synthesis and export machineries by the Hog1 SAPK. |
format | Online Article Text |
id | pubmed-3682539 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-36825392013-06-17 The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress Regot, Sergi de Nadal, Eulàlia Rodríguez-Navarro, Susana González-Novo, Alberto Pérez-Fernandez, Jorge Gadal, Olivier Seisenbacher, Gerhard Ammerer, Gustav Posas, Francesc J Biol Chem Gene Regulation The control of mRNA biogenesis is exerted at several steps. In response to extracellular stimuli, stress-activated protein kinases (SAPK) modulate gene expression to maximize cell survival. In yeast, the Hog1 SAPK plays a key role in reprogramming the gene expression pattern required for cell survival upon osmostress by acting during transcriptional initiation and elongation. Here, we genetically show that an intact nuclear pore complex is important for cell survival and maximal expression of stress-responsive genes. The Hog1 SAPK associates with nuclear pore complex components and directly phosphorylates the Nup1, Nup2, and Nup60 components of the inner nuclear basket. Mutation of those factors resulted in a deficient export of stress-responsive genes upon stress. Association of Nup1, Nup2, and Nup60 to stress-responsive promoters occurs upon stress depending on Hog1 activity. Accordingly, STL1 gene territory is maintained at the nuclear periphery upon osmostress in a Hog1-dependent manner. Cells containing non-phosphorylatable mutants in Nup1 or Nup2 display reduced expression of stress-responsive genes. Together, proper mRNA biogenesis of stress-responsive genes requires of the coordinate action of synthesis and export machineries by the Hog1 SAPK. American Society for Biochemistry and Molecular Biology 2013-06-14 2013-05-03 /pmc/articles/PMC3682539/ /pubmed/23645671 http://dx.doi.org/10.1074/jbc.M112.444042 Text en © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access. Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/) applies to Author Choice Articles |
spellingShingle | Gene Regulation Regot, Sergi de Nadal, Eulàlia Rodríguez-Navarro, Susana González-Novo, Alberto Pérez-Fernandez, Jorge Gadal, Olivier Seisenbacher, Gerhard Ammerer, Gustav Posas, Francesc The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress |
title | The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress |
title_full | The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress |
title_fullStr | The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress |
title_full_unstemmed | The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress |
title_short | The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress |
title_sort | hog1 stress-activated protein kinase targets nucleoporins to control mrna export upon stress |
topic | Gene Regulation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3682539/ https://www.ncbi.nlm.nih.gov/pubmed/23645671 http://dx.doi.org/10.1074/jbc.M112.444042 |
work_keys_str_mv | AT regotsergi thehog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT denadaleulalia thehog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT rodrigueznavarrosusana thehog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT gonzaleznovoalberto thehog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT perezfernandezjorge thehog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT gadalolivier thehog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT seisenbachergerhard thehog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT ammerergustav thehog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT posasfrancesc thehog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT regotsergi hog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT denadaleulalia hog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT rodrigueznavarrosusana hog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT gonzaleznovoalberto hog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT perezfernandezjorge hog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT gadalolivier hog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT seisenbachergerhard hog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT ammerergustav hog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress AT posasfrancesc hog1stressactivatedproteinkinasetargetsnucleoporinstocontrolmrnaexportuponstress |