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The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress

The control of mRNA biogenesis is exerted at several steps. In response to extracellular stimuli, stress-activated protein kinases (SAPK) modulate gene expression to maximize cell survival. In yeast, the Hog1 SAPK plays a key role in reprogramming the gene expression pattern required for cell surviv...

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Autores principales: Regot, Sergi, de Nadal, Eulàlia, Rodríguez-Navarro, Susana, González-Novo, Alberto, Pérez-Fernandez, Jorge, Gadal, Olivier, Seisenbacher, Gerhard, Ammerer, Gustav, Posas, Francesc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2013
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3682539/
https://www.ncbi.nlm.nih.gov/pubmed/23645671
http://dx.doi.org/10.1074/jbc.M112.444042
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author Regot, Sergi
de Nadal, Eulàlia
Rodríguez-Navarro, Susana
González-Novo, Alberto
Pérez-Fernandez, Jorge
Gadal, Olivier
Seisenbacher, Gerhard
Ammerer, Gustav
Posas, Francesc
author_facet Regot, Sergi
de Nadal, Eulàlia
Rodríguez-Navarro, Susana
González-Novo, Alberto
Pérez-Fernandez, Jorge
Gadal, Olivier
Seisenbacher, Gerhard
Ammerer, Gustav
Posas, Francesc
author_sort Regot, Sergi
collection PubMed
description The control of mRNA biogenesis is exerted at several steps. In response to extracellular stimuli, stress-activated protein kinases (SAPK) modulate gene expression to maximize cell survival. In yeast, the Hog1 SAPK plays a key role in reprogramming the gene expression pattern required for cell survival upon osmostress by acting during transcriptional initiation and elongation. Here, we genetically show that an intact nuclear pore complex is important for cell survival and maximal expression of stress-responsive genes. The Hog1 SAPK associates with nuclear pore complex components and directly phosphorylates the Nup1, Nup2, and Nup60 components of the inner nuclear basket. Mutation of those factors resulted in a deficient export of stress-responsive genes upon stress. Association of Nup1, Nup2, and Nup60 to stress-responsive promoters occurs upon stress depending on Hog1 activity. Accordingly, STL1 gene territory is maintained at the nuclear periphery upon osmostress in a Hog1-dependent manner. Cells containing non-phosphorylatable mutants in Nup1 or Nup2 display reduced expression of stress-responsive genes. Together, proper mRNA biogenesis of stress-responsive genes requires of the coordinate action of synthesis and export machineries by the Hog1 SAPK.
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spelling pubmed-36825392013-06-17 The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress Regot, Sergi de Nadal, Eulàlia Rodríguez-Navarro, Susana González-Novo, Alberto Pérez-Fernandez, Jorge Gadal, Olivier Seisenbacher, Gerhard Ammerer, Gustav Posas, Francesc J Biol Chem Gene Regulation The control of mRNA biogenesis is exerted at several steps. In response to extracellular stimuli, stress-activated protein kinases (SAPK) modulate gene expression to maximize cell survival. In yeast, the Hog1 SAPK plays a key role in reprogramming the gene expression pattern required for cell survival upon osmostress by acting during transcriptional initiation and elongation. Here, we genetically show that an intact nuclear pore complex is important for cell survival and maximal expression of stress-responsive genes. The Hog1 SAPK associates with nuclear pore complex components and directly phosphorylates the Nup1, Nup2, and Nup60 components of the inner nuclear basket. Mutation of those factors resulted in a deficient export of stress-responsive genes upon stress. Association of Nup1, Nup2, and Nup60 to stress-responsive promoters occurs upon stress depending on Hog1 activity. Accordingly, STL1 gene territory is maintained at the nuclear periphery upon osmostress in a Hog1-dependent manner. Cells containing non-phosphorylatable mutants in Nup1 or Nup2 display reduced expression of stress-responsive genes. Together, proper mRNA biogenesis of stress-responsive genes requires of the coordinate action of synthesis and export machineries by the Hog1 SAPK. American Society for Biochemistry and Molecular Biology 2013-06-14 2013-05-03 /pmc/articles/PMC3682539/ /pubmed/23645671 http://dx.doi.org/10.1074/jbc.M112.444042 Text en © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access. Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/) applies to Author Choice Articles
spellingShingle Gene Regulation
Regot, Sergi
de Nadal, Eulàlia
Rodríguez-Navarro, Susana
González-Novo, Alberto
Pérez-Fernandez, Jorge
Gadal, Olivier
Seisenbacher, Gerhard
Ammerer, Gustav
Posas, Francesc
The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress
title The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress
title_full The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress
title_fullStr The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress
title_full_unstemmed The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress
title_short The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress
title_sort hog1 stress-activated protein kinase targets nucleoporins to control mrna export upon stress
topic Gene Regulation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3682539/
https://www.ncbi.nlm.nih.gov/pubmed/23645671
http://dx.doi.org/10.1074/jbc.M112.444042
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