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Isolation and Characterization of a Primary Proximal Tubular Epithelial Cell Model from Human Kidney by CD10/CD13 Double Labeling
Renal proximal tubular epithelial cells play a central role in renal physiology and are among the cell types most sensitive to ischemia and xenobiotic nephrotoxicity. In order to investigate the molecular and cellular mechanisms underlying the pathophysiology of kidney injuries, a stable and well-ch...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3682988/ https://www.ncbi.nlm.nih.gov/pubmed/23799132 http://dx.doi.org/10.1371/journal.pone.0066750 |
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author | Van der Hauwaert, Cynthia Savary, Grégoire Gnemmi, Viviane Glowacki, François Pottier, Nicolas Bouillez, Audrey Maboudou, Patrice Zini, Laurent Leroy, Xavier Cauffiez, Christelle Perrais, Michaël Aubert, Sébastien |
author_facet | Van der Hauwaert, Cynthia Savary, Grégoire Gnemmi, Viviane Glowacki, François Pottier, Nicolas Bouillez, Audrey Maboudou, Patrice Zini, Laurent Leroy, Xavier Cauffiez, Christelle Perrais, Michaël Aubert, Sébastien |
author_sort | Van der Hauwaert, Cynthia |
collection | PubMed |
description | Renal proximal tubular epithelial cells play a central role in renal physiology and are among the cell types most sensitive to ischemia and xenobiotic nephrotoxicity. In order to investigate the molecular and cellular mechanisms underlying the pathophysiology of kidney injuries, a stable and well-characterized primary culture model of proximal tubular cells is required. An existing model of proximal tubular cells is hampered by the cellular heterogeneity of kidney; a method based on cell sorting for specific markers must therefore be developed. In this study, we present a primary culture model based on the mechanical and enzymatic dissociation of healthy tissue obtained from nephrectomy specimens. Renal epithelial cells were sorted using co-labeling for CD10 and CD13, two renal proximal tubular epithelial markers, by flow cytometry. Their purity, phenotypic stability and functional properties were evaluated over several passages. Our results demonstrate that CD10/CD13 double-positive cells constitute a pure, functional and stable proximal tubular epithelial cell population that displays proximal tubule markers and epithelial characteristics over the long term, whereas cells positive for either CD10 or CD13 alone appear to be heterogeneous. In conclusion, this study describes a method for establishing a robust renal proximal tubular epithelial cell model suitable for further experimentation. |
format | Online Article Text |
id | pubmed-3682988 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36829882013-06-24 Isolation and Characterization of a Primary Proximal Tubular Epithelial Cell Model from Human Kidney by CD10/CD13 Double Labeling Van der Hauwaert, Cynthia Savary, Grégoire Gnemmi, Viviane Glowacki, François Pottier, Nicolas Bouillez, Audrey Maboudou, Patrice Zini, Laurent Leroy, Xavier Cauffiez, Christelle Perrais, Michaël Aubert, Sébastien PLoS One Research Article Renal proximal tubular epithelial cells play a central role in renal physiology and are among the cell types most sensitive to ischemia and xenobiotic nephrotoxicity. In order to investigate the molecular and cellular mechanisms underlying the pathophysiology of kidney injuries, a stable and well-characterized primary culture model of proximal tubular cells is required. An existing model of proximal tubular cells is hampered by the cellular heterogeneity of kidney; a method based on cell sorting for specific markers must therefore be developed. In this study, we present a primary culture model based on the mechanical and enzymatic dissociation of healthy tissue obtained from nephrectomy specimens. Renal epithelial cells were sorted using co-labeling for CD10 and CD13, two renal proximal tubular epithelial markers, by flow cytometry. Their purity, phenotypic stability and functional properties were evaluated over several passages. Our results demonstrate that CD10/CD13 double-positive cells constitute a pure, functional and stable proximal tubular epithelial cell population that displays proximal tubule markers and epithelial characteristics over the long term, whereas cells positive for either CD10 or CD13 alone appear to be heterogeneous. In conclusion, this study describes a method for establishing a robust renal proximal tubular epithelial cell model suitable for further experimentation. Public Library of Science 2013-06-14 /pmc/articles/PMC3682988/ /pubmed/23799132 http://dx.doi.org/10.1371/journal.pone.0066750 Text en © 2013 Van der Hauwaert et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Van der Hauwaert, Cynthia Savary, Grégoire Gnemmi, Viviane Glowacki, François Pottier, Nicolas Bouillez, Audrey Maboudou, Patrice Zini, Laurent Leroy, Xavier Cauffiez, Christelle Perrais, Michaël Aubert, Sébastien Isolation and Characterization of a Primary Proximal Tubular Epithelial Cell Model from Human Kidney by CD10/CD13 Double Labeling |
title | Isolation and Characterization of a Primary Proximal Tubular Epithelial Cell Model from Human Kidney by CD10/CD13 Double Labeling |
title_full | Isolation and Characterization of a Primary Proximal Tubular Epithelial Cell Model from Human Kidney by CD10/CD13 Double Labeling |
title_fullStr | Isolation and Characterization of a Primary Proximal Tubular Epithelial Cell Model from Human Kidney by CD10/CD13 Double Labeling |
title_full_unstemmed | Isolation and Characterization of a Primary Proximal Tubular Epithelial Cell Model from Human Kidney by CD10/CD13 Double Labeling |
title_short | Isolation and Characterization of a Primary Proximal Tubular Epithelial Cell Model from Human Kidney by CD10/CD13 Double Labeling |
title_sort | isolation and characterization of a primary proximal tubular epithelial cell model from human kidney by cd10/cd13 double labeling |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3682988/ https://www.ncbi.nlm.nih.gov/pubmed/23799132 http://dx.doi.org/10.1371/journal.pone.0066750 |
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