Murine Cell Glycolipids Customization by Modular Expression of Glycosyltransferases

Functional analysis of glycolipids has been hampered by their complex nature and combinatorial expression in cells and tissues. We report an efficient and easy method to generate cells with specific glycolipids. In our proof of principle experiments we have demonstrated the customized expression of...

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Detalles Bibliográficos
Autores principales: Cid, Emili, Yamamoto, Miyako, Buschbeck, Marcus, Yamamoto, Fumiichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3683021/
https://www.ncbi.nlm.nih.gov/pubmed/23798992
http://dx.doi.org/10.1371/journal.pone.0064728
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author Cid, Emili
Yamamoto, Miyako
Buschbeck, Marcus
Yamamoto, Fumiichiro
author_facet Cid, Emili
Yamamoto, Miyako
Buschbeck, Marcus
Yamamoto, Fumiichiro
author_sort Cid, Emili
collection PubMed
description Functional analysis of glycolipids has been hampered by their complex nature and combinatorial expression in cells and tissues. We report an efficient and easy method to generate cells with specific glycolipids. In our proof of principle experiments we have demonstrated the customized expression of two relevant glycosphingolipids on murine fibroblasts, stage-specific embryonic antigen 3 (SSEA-3), a marker for stem cells, and Forssman glycolipid, a xenoantigen. Sets of genes encoding glycosyltansferases were transduced by viral infection followed by multi-color cell sorting based on coupled expression of fluorescent proteins.
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spelling pubmed-36830212013-06-24 Murine Cell Glycolipids Customization by Modular Expression of Glycosyltransferases Cid, Emili Yamamoto, Miyako Buschbeck, Marcus Yamamoto, Fumiichiro PLoS One Research Article Functional analysis of glycolipids has been hampered by their complex nature and combinatorial expression in cells and tissues. We report an efficient and easy method to generate cells with specific glycolipids. In our proof of principle experiments we have demonstrated the customized expression of two relevant glycosphingolipids on murine fibroblasts, stage-specific embryonic antigen 3 (SSEA-3), a marker for stem cells, and Forssman glycolipid, a xenoantigen. Sets of genes encoding glycosyltansferases were transduced by viral infection followed by multi-color cell sorting based on coupled expression of fluorescent proteins. Public Library of Science 2013-06-14 /pmc/articles/PMC3683021/ /pubmed/23798992 http://dx.doi.org/10.1371/journal.pone.0064728 Text en © 2013 Cid et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cid, Emili
Yamamoto, Miyako
Buschbeck, Marcus
Yamamoto, Fumiichiro
Murine Cell Glycolipids Customization by Modular Expression of Glycosyltransferases
title Murine Cell Glycolipids Customization by Modular Expression of Glycosyltransferases
title_full Murine Cell Glycolipids Customization by Modular Expression of Glycosyltransferases
title_fullStr Murine Cell Glycolipids Customization by Modular Expression of Glycosyltransferases
title_full_unstemmed Murine Cell Glycolipids Customization by Modular Expression of Glycosyltransferases
title_short Murine Cell Glycolipids Customization by Modular Expression of Glycosyltransferases
title_sort murine cell glycolipids customization by modular expression of glycosyltransferases
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3683021/
https://www.ncbi.nlm.nih.gov/pubmed/23798992
http://dx.doi.org/10.1371/journal.pone.0064728
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