Postsynaptic current bursts instruct action potential firing at a graded synapse

Nematode neurons generally produce graded potentials instead of action potentials (APs). It is unclear how the graded potentials control postsynaptic cells under physiological conditions. Here we show that postsynaptic currents (PSCs) frequently occur in bursts at the neuromuscular junction of C. elegans. Cholinergic bursts concur with facilitated AP firing, elevated cytosolic [Ca(2+)], and contraction of the muscle whereas GABA ergic bursts suppress AP firing. The bursts, distinct from artificially evoked responses, are characterized by a persistent current (the primary component of burst-associated charge transfer)and increased frequency and mean amplitude of PSC events. The persistent current of cholinergic PSC bursts is mostly mediated by levamisole-sensitive acetylcholine receptors, which correlates well with locomotory phenotypes of receptor mutants. Eliminating command interneurons abolishes the bursts whereas mutating SLO-1 K(+) channel, a potent presynaptic inhibitor of exocytosis, greatly increases the mean burst duration. These observations suggest that motoneurons control muscle by producing PSC bursts.