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Optofluidic realization and retaining of cell–cell contact using an abrupt tapered optical fibre

Studies reveal that there exists much interaction and communication between bacterial cells, with parts of these social behaviors depending on cell–cell contacts. The cell–cell contact has proved to be crucial for determining various biochemical processes. However, for cell culture with relatively l...

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Autores principales: Xin, Hongbao, Zhang, Yao, Lei, Hongxiang, Li, Yayi, Zhang, Huixian, Li, Baojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3683673/
https://www.ncbi.nlm.nih.gov/pubmed/23771190
http://dx.doi.org/10.1038/srep01993
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author Xin, Hongbao
Zhang, Yao
Lei, Hongxiang
Li, Yayi
Zhang, Huixian
Li, Baojun
author_facet Xin, Hongbao
Zhang, Yao
Lei, Hongxiang
Li, Yayi
Zhang, Huixian
Li, Baojun
author_sort Xin, Hongbao
collection PubMed
description Studies reveal that there exists much interaction and communication between bacterial cells, with parts of these social behaviors depending on cell–cell contacts. The cell–cell contact has proved to be crucial for determining various biochemical processes. However, for cell culture with relatively low cell concentration, it is difficult to precisely control and retain the contact of a small group of cells. Particularly, the retaining of cell–cell contact is difficult when flows occur in the medium. Here, we report an optofluidic method for realization and retaining of Escherichia coli cell–cell contact in a microfluidic channel using an abrupt tapered optical fibre. The contact process is based on launching a 980-nm wavelength laser into the fibre, E. coli cells were trapped onto the fibre tip one after another, retaining cell–cell contact and forming a highly organized cell chain. The formed chains further show the ability as bio-optical waveguides.
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spelling pubmed-36836732013-06-24 Optofluidic realization and retaining of cell–cell contact using an abrupt tapered optical fibre Xin, Hongbao Zhang, Yao Lei, Hongxiang Li, Yayi Zhang, Huixian Li, Baojun Sci Rep Article Studies reveal that there exists much interaction and communication between bacterial cells, with parts of these social behaviors depending on cell–cell contacts. The cell–cell contact has proved to be crucial for determining various biochemical processes. However, for cell culture with relatively low cell concentration, it is difficult to precisely control and retain the contact of a small group of cells. Particularly, the retaining of cell–cell contact is difficult when flows occur in the medium. Here, we report an optofluidic method for realization and retaining of Escherichia coli cell–cell contact in a microfluidic channel using an abrupt tapered optical fibre. The contact process is based on launching a 980-nm wavelength laser into the fibre, E. coli cells were trapped onto the fibre tip one after another, retaining cell–cell contact and forming a highly organized cell chain. The formed chains further show the ability as bio-optical waveguides. Nature Publishing Group 2013-06-17 /pmc/articles/PMC3683673/ /pubmed/23771190 http://dx.doi.org/10.1038/srep01993 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Xin, Hongbao
Zhang, Yao
Lei, Hongxiang
Li, Yayi
Zhang, Huixian
Li, Baojun
Optofluidic realization and retaining of cell–cell contact using an abrupt tapered optical fibre
title Optofluidic realization and retaining of cell–cell contact using an abrupt tapered optical fibre
title_full Optofluidic realization and retaining of cell–cell contact using an abrupt tapered optical fibre
title_fullStr Optofluidic realization and retaining of cell–cell contact using an abrupt tapered optical fibre
title_full_unstemmed Optofluidic realization and retaining of cell–cell contact using an abrupt tapered optical fibre
title_short Optofluidic realization and retaining of cell–cell contact using an abrupt tapered optical fibre
title_sort optofluidic realization and retaining of cell–cell contact using an abrupt tapered optical fibre
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3683673/
https://www.ncbi.nlm.nih.gov/pubmed/23771190
http://dx.doi.org/10.1038/srep01993
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