Cargando…
RNA localization in Xenopus oocytes uses a core group of trans-acting factors irrespective of destination
The 3′ untranslated region of mRNA encoding PHAX, a phosphoprotein required for nuclear export of U-type snRNAs, contains cis-acting sequence motifs E2 and VM1 that are required for localization of RNAs to the vegetal hemisphere of Xenopus oocytes. However, we have found that PHAX mRNA is transporte...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3683923/ https://www.ncbi.nlm.nih.gov/pubmed/23645708 http://dx.doi.org/10.1261/rna.038232.113 |
_version_ | 1782273529577734144 |
---|---|
author | Snedden, Donald D. Bertke, Michelle M. Vernon, Dominic Huber, Paul W. |
author_facet | Snedden, Donald D. Bertke, Michelle M. Vernon, Dominic Huber, Paul W. |
author_sort | Snedden, Donald D. |
collection | PubMed |
description | The 3′ untranslated region of mRNA encoding PHAX, a phosphoprotein required for nuclear export of U-type snRNAs, contains cis-acting sequence motifs E2 and VM1 that are required for localization of RNAs to the vegetal hemisphere of Xenopus oocytes. However, we have found that PHAX mRNA is transported to the opposite, animal, hemisphere. A set of proteins that cross-link to the localization elements of vegetally localized RNAs are also cross-linked to PHAX and An1 mRNAs, demonstrating that the composition of RNP complexes that form on these localization elements is highly conserved irrespective of the final destination of the RNA. The ability of RNAs to bind this core group of proteins is correlated with localization activity. Staufen1, which binds to Vg1 and VegT mRNAs, is not associated with RNAs localized to the animal hemisphere and may determine, at least in part, the direction of RNA movement in Xenopus oocytes. |
format | Online Article Text |
id | pubmed-3683923 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-36839232014-07-01 RNA localization in Xenopus oocytes uses a core group of trans-acting factors irrespective of destination Snedden, Donald D. Bertke, Michelle M. Vernon, Dominic Huber, Paul W. RNA Reports The 3′ untranslated region of mRNA encoding PHAX, a phosphoprotein required for nuclear export of U-type snRNAs, contains cis-acting sequence motifs E2 and VM1 that are required for localization of RNAs to the vegetal hemisphere of Xenopus oocytes. However, we have found that PHAX mRNA is transported to the opposite, animal, hemisphere. A set of proteins that cross-link to the localization elements of vegetally localized RNAs are also cross-linked to PHAX and An1 mRNAs, demonstrating that the composition of RNP complexes that form on these localization elements is highly conserved irrespective of the final destination of the RNA. The ability of RNAs to bind this core group of proteins is correlated with localization activity. Staufen1, which binds to Vg1 and VegT mRNAs, is not associated with RNAs localized to the animal hemisphere and may determine, at least in part, the direction of RNA movement in Xenopus oocytes. Cold Spring Harbor Laboratory Press 2013-07 /pmc/articles/PMC3683923/ /pubmed/23645708 http://dx.doi.org/10.1261/rna.038232.113 Text en © 2013; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/. |
spellingShingle | Reports Snedden, Donald D. Bertke, Michelle M. Vernon, Dominic Huber, Paul W. RNA localization in Xenopus oocytes uses a core group of trans-acting factors irrespective of destination |
title | RNA localization in Xenopus oocytes uses a core group of trans-acting factors irrespective of destination |
title_full | RNA localization in Xenopus oocytes uses a core group of trans-acting factors irrespective of destination |
title_fullStr | RNA localization in Xenopus oocytes uses a core group of trans-acting factors irrespective of destination |
title_full_unstemmed | RNA localization in Xenopus oocytes uses a core group of trans-acting factors irrespective of destination |
title_short | RNA localization in Xenopus oocytes uses a core group of trans-acting factors irrespective of destination |
title_sort | rna localization in xenopus oocytes uses a core group of trans-acting factors irrespective of destination |
topic | Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3683923/ https://www.ncbi.nlm.nih.gov/pubmed/23645708 http://dx.doi.org/10.1261/rna.038232.113 |
work_keys_str_mv | AT sneddendonaldd rnalocalizationinxenopusoocytesusesacoregroupoftransactingfactorsirrespectiveofdestination AT bertkemichellem rnalocalizationinxenopusoocytesusesacoregroupoftransactingfactorsirrespectiveofdestination AT vernondominic rnalocalizationinxenopusoocytesusesacoregroupoftransactingfactorsirrespectiveofdestination AT huberpaulw rnalocalizationinxenopusoocytesusesacoregroupoftransactingfactorsirrespectiveofdestination |