Thermostable group II intron reverse transcriptase fusion proteins and their use in cDNA synthesis and next-generation RNA sequencing

Mobile group II introns encode reverse transcriptases (RTs) that function in intron mobility (“retrohoming”) by a process that requires reverse transcription of a highly structured, 2–2.5-kb intron RNA with high processivity and fidelity. Although the latter properties are potentially useful for app...

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Detalles Bibliográficos
Autores principales: Mohr, Sabine, Ghanem, Eman, Smith, Whitney, Sheeter, Dennis, Qin, Yidan, King, Olga, Polioudakis, Damon, Iyer, Vishwanath R., Hunicke-Smith, Scott, Swamy, Sajani, Kuersten, Scott, Lambowitz, Alan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2013
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3683930/
https://www.ncbi.nlm.nih.gov/pubmed/23697550
http://dx.doi.org/10.1261/rna.039743.113

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