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A comparative study of microbial diversity and community structure in marine sediments using poly(A) tailing and reverse transcription-PCR
To obtain a better understanding of metabolically active microbial communities, we tested a molecular ecological approach using poly(A) tailing of environmental 16S rRNA, followed by full-length complementary DNA (cDNA) synthesis and sequencing to eliminate potential biases caused by mismatching of...
Autores principales: | Hoshino, Tatsuhiko, Inagaki, Fumio |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684792/ https://www.ncbi.nlm.nih.gov/pubmed/23785366 http://dx.doi.org/10.3389/fmicb.2013.00160 |
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